中国畜牧兽医 ›› 2026, Vol. 53 ›› Issue (2): 682-691.doi: 10.16431/j.cnki.1671-7236.2026.02.016

• 营养与饲料 • 上一篇    下一篇

植物乳杆菌硒对蛋鸡卵巢及输卵管组织形态、抗氧化功能和炎症指标的影响

王慧欣1,2(), 高庆涛1,3, 黎家恒1, 张顺芬1, 钟儒清1(), 王阳2(), 陈亮1, 张宏福1   

  1. 1.中国农业科学院北京畜牧兽医研究所,畜禽营养与饲养全国重点实验室,北京 100193
    2.青岛农业大学动物科技学院,青岛 266109
    3.山东省农业科学院家禽研究所,济南 250100
  • 收稿日期:2025-05-08 出版日期:2026-02-20 发布日期:2026-01-27
  • 通讯作者: 钟儒清,王阳 E-mail:wanghuixint9@163.com;zhongruqing@caas.cn;yangwang@qau.edu.cn
  • 作者简介:王慧欣,E-mail:wanghuixint9@163.com
  • 基金资助:
    国家重点研发计划课题(2022YFD1300705);中国农业科学院科技创新工程(ASTIP-IAS07)

Effects of Lactobacillus plantarum Selenium on Tissue Morphology, Antioxidant Function and Inflammatory Indices in Ovary and Oviduct of Laying Hens

WANG Huixin1,2(), GAO Qingtao1,3, LI Jiaheng1, ZHANG Shunfen1, ZHONG Ruqing1(), WANG Yang2(), CHEN Liang1, ZHANG Hongfu1   

  1. 1.State Key Laboratory of Animal Nutrition and Feeding,Institute of Animal Science,Chinese Academy of Agricultural Sciences,Beijing 100193,China
    2.College of Animal Science and Technology,Qingdao Agricultural University,Qingdao 266109,China
    3.Poultry Institute,Shandong Academy of Agricultural Sciences,Jinan 250100,China
  • Received:2025-05-08 Online:2026-02-20 Published:2026-01-27
  • Contact: ZHONG Ruqing, WANG Yang E-mail:wanghuixint9@163.com;zhongruqing@caas.cn;yangwang@qau.edu.cn

摘要:

目的 研究植物乳杆菌硒对蛋鸡卵巢、输卵管组织结构及抗氧化功能和炎症指标的影响,以期为蛋鸡生殖健康提供新的硒源参考。 方法 试验选取96只22周龄健康海兰褐蛋鸡,随机分为2个处理组(每组16个重复,每个重复3只鸡):对照组(CON)蛋鸡饲喂常规玉米-豆粕型基础饲粮,饲粮中硒实测含量为0.38 mg/kg;植物乳杆菌硒组(SEL)在基础饲粮基础上添加500 mg/kg植物乳杆菌硒,饲粮中硒实测含量为1.58 mg/kg。试验共188 d。试验结束后每组随机挑选8只蛋鸡屠宰,采集输卵管和卵巢组织样本,分析植物乳杆菌硒对蛋鸡生产性能的影响;通过HE染色观察卵巢和输卵管的组织结构,并进一步检测植物乳杆菌硒对蛋鸡卵巢激素水平的影响;通过实时荧光定量PCR检测卵巢和输卵管组织中抗氧化与炎症相关基因的相对表达量。 结果 ①两组蛋鸡日采食量、产蛋率、平均日产蛋重、料蛋比等主要生产性能指标均无显著差异(P>0.05)。②与CON组相比,SEL组蛋鸡卵巢组织形态更为完整,大黄卵泡数量显著增加(P<0.05),且卵巢组织中促卵泡激素(FSH)、雌二醇(E2)水平均显著升高(P<0.05)。③与CON组相比,SEL蛋鸡输卵管膨大部的组织结构和形态更完整,输卵管膨大部组织中抗氧化相关基因GPX1、GPX4、CAT、TXNRD2、TXNRD3和SOD1,以及炎症因子相关基因IL-6和TNFAIP3表达量均显著升高(P<0.05)。 结论 饲粮中添加植物乳杆菌硒可有效改善产蛋鸡卵巢及输卵管组织的形态结构,提升输卵管组织的抗氧化功能。本试验结果表明植物乳杆菌硒对提升蛋鸡输卵管健康、提高蛋鸡综合生产效益具有重要应用价值。

关键词: 蛋鸡; 植物乳杆菌硒; 卵巢; 输卵管; 抗氧化功能

Abstract:

Objective This study aimed to investigate the effects of Lactobacillus plantarum selenium on the ovarian and oviduct structure, as well as the antioxidant function and inflammatory indices of laying hens,in order to provide a new reference for selenium sources in maintaining the reproductive health of laying hens. Method A total of 96 healthy 22-week-old Hy-Line Brown laying hens were randomly divided into 2 treatment groups, with 16 replicates per group and 3 hens per replicate. Hens in control group (CON) was fed a conventional corn-soybean meal-based diet for laying hens, with a measured selenium content of 0.38 mg/kg. The Lactobacillus plantarum selenium group (SEL) was supplemented with 500 mg/kg of Lactobacillus plantarum selenium product on the basis of the basal diet, and the measured selenium content in the diet was 1.58 mg/kg. The experiment lasted for 188 days. At the end of the experiment, 8 laying hens were randomly selected from each group for slaughter. Oviduct and ovary tissue samples were collected to analyze the effects of Lactobacillus plantarum selenium on the production performance of laying hens. The tissue structure of ovaries and oviducts was observed by HE staining, and the effect of Lactobacillus plantarum selenium on the ovarian hormone levels of laying hens was further examined. Real-time quantitative PCR was performed to determine the relative expression of antioxidant and inflammation-related genes in ovarian and oviduct. Result ①There were no significant differences in the main production performance indicators such as daily feed intake, egg production rate, average daily egg weight, and feed-egg ratio between the two groups of laying hens (P>0.05). ② Compared with CON group, the ovarian of SEL group was more intact, and the number of large yellow follicles significantly increased (P<0.05). Moreover, the levels of follicle-stimulating hormone (FSH) and estradiol (E2) in ovarian were significantly increased (P<0.05).③ Compared with CON group, the tissue structure and morphology of the magnum of oviduct in SEL group were more complete. The expression of antioxidant-related genes GPX1, GPX4, CATTXNRD2, TXNRD3 and SOD1, as well as inflammatory factor-related genes IL-6 and TNFAIP3 in the magnum tissue of oviduct were significantly increased (P<0.05). Conclusion The supplementation of feed with Lactobacillus plantarum selenium could improve the morphological structure of ovaries and oviduct tissues in laying hens, and enhance the antioxidant function of oviduct tissues. The results of this experiment indicated that Lactobacillus plantarum selenium has significant application value in improving the health of oviducts and enhancing the overall production efficiency of laying hens.

Key words: laying hens; Lactobacillus plantarum selenium; ovary; oviduct; antioxidant function

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