美系大白猪PTER和CALB2基因多态性与生长性状的关联分析

doi:10.16431/j.cnki.1671-7236.2026.02.031

Abstract

Abstract:

Objective This study was aimed to investigate the correlation between phosphotriesterase related gene （PTER） and calbindin 2 （CALB2） gene polymorphisms and growth traits in American Yorkshire pigs， aiming provide new marker resources for molecular breeding of growth traits in this breed. Methods A total of 385 American Yorkshire pigs reaching 100 kg body weight were selected. Backfat thickness was measured， and ear tissue samples were collected for DNA extraction. Primers were designed based on the reference porcine genome sequence （Sscrofa 11.1）. The single nucleotide polymorphism （SNP） of PTER and CALB2 genes was identified through direct sequencing of PCR products. Genetic diversity analysis of SNP was performed using POPGENE software， and correlations with growth traits in American Yorkshire pigs were analyzed using SPSS 23.0 software. Result Polymorphism detection results revealed that there were 3 genotypes （GG， GC， and CC） at g.45384562 G>C of PTER gene in American Yorkshire pigs， the dominant genotype was GG （0.5403）， with a dominant allele was G（0.7468）. There were 3 genotypes （GG， GA， and AA） at g.14391325 G>Aof CALB2 gene， with GA as the dominant allele （0.4545） and A as the dominant allele frequency （0.5545）. Population genetic analysis results indicated that the polymorphic information content （PIC） of 2 SNPs of PTER and CALB2 genes was 0.31 and 0.37， respectively， representing moderate polymorphism （0.25<PIC<0.5）. Phenotype association analysis results revealed that g.45384562 G>C of PTER gene had no significant effect on the corrected days to 100 kg in American Yorkshire pigs （P>0.05）. However， the corrected live backfat thickness at 100 kg of GG genotype was significantly higher than that of GC and CC genotypes （P<0.05）. g.14391325 G>A of CALB2 gene was extremely significantly associated with both the corrected days to 100 kg and corrected live backfat thickness at 100 kg in American Yorkshire pigs （P<0.01）. Combined effect analysis indicated that CCAA genotype （combining PTER gene CC genotype and CALB2 gene AA genotype） represented an optimal genotype combination associated with shorter corrected days to 100 kg and reduced corrected live backfat thickness at 100 kg， with corresponding values for both traits being lower than those in other individuals. Conclusion This study identified 2 SNPs of PTER and CALB2 genes and confirmed their significant association with growth traits in American Yorkshire pigs. The results suggested that 2 SNPs （particularly the CCAA combined genotype） hold potential for genetic improvement in American Yorkshire pigs.

Key words: American Yorkshire pigs; PTER gene; CALB2 gene; corrected days to 100 kg; corrected live backfat thickness at 100 kg

CLC Number:

S813.3

XU Juan, ZHANG Huibin, ZHANG Xiaodong, LI Qinggang. Association Analysis of PTER and CALB2 Gene Polymorphisms with Growth Traits in American Yorkshire pigs[J]. China Animal Husbandry & Veterinary Medicine, 2026, 53(2): 859-869.

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References 31

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基因 Genes	引物序列 Primer sequences （5′→3′）	片段长度 Fragment length/bp	退火温度 Annealing temperature/℃
PTER	F： GTCAAGTTCATAAGTTTTGGCAC	719	58.5
PTER	R： TAGGGCAAGCAGGAAGAGTCAGC	719	58.5
CALB2	F： TGGTGTTCCTCTCTCCCCTG	548	62.8
CALB2	R： CCACTGTCCTCTCGTGTTGA	548	62.8

基因 Genes	基因型频率 Genotype frequency			等位基因频率 Allele frequency		χ²值 χ²value	P值 P-value
PTER	GG	GC	CC	G	C
	0.5403（208）	0.4130（159）	0.0468（18）	0.7468	0.2532	3.2520	0.0713
CALB2	GG	GA	AA	G	A
	0.2182（84）	0.4545（175）	0.3273（126）	0.4455	0.5545	2.4615	0.1167

基因 Genes	多态信息含量 PIC	杂合度 He	有效等位基因数 Ne
PTER	0.31	0.38	1.61
CALB2	0.37	0.49	1.97

合并基因型 Combined genotypes	样本量 N/头	校正100 kg日龄 Corrected days to 100 kg	显著性检验 Significance test
合并基因型 Combined genotypes	样本量 N/头	校正100 kg日龄 Corrected days to 100 kg	GGGG	GGGA	GGAA	GCGG	GCGA	GCAA	CCGG	CCGA	CCAA
GGGG	41	169.1±6.8		0.000^**	0.000^**	0.003^**	0.000^**	0.000^**	0.257	0.023^*	0.000^**
GGGA	99	162.9±8.9	0.000^**		0.001^**	0.756	0.519	0.010^**	0.932	0.675	0.035^*
GGAA	68	158.3±7.7	0.000^**	0.001^**		0.002^**	0.009^**	0.575	0.311	0.292	0.483
GCGG	40	163.4±7.8	0.003^**	0.756	0.002^**		0.423	0.018^*	0.989	0.583	0.031^*
GCGA	68	162.1±8.9	0.000^**	0.519	0.009^**	0.423		0.065	0.797	0.888	0.068
GCAA	51	159.2±9.0	0.000^**	0.010^**	0.575	0.018^*	0.065		0.405	0.445	0.343
CCGG	3	163.3±2.8	0.257	0.932	0.311	0.989	0.797	0.405		0.763	0.204
CCGA	8	161.6±12.7	0.023^*	0.675	0.292	0.583	0.888	0.445	0.763		0.194
CCAA	7	156.0±5.4	0.000^**	0.035^*	0.483	0.031^*	0.068	0.343	0.204	0.194

合并基因型 Combined genotypes	样本量 N/头	校正100 kg 活体背膘厚 Corrected live backfat thickness at 100 kg/mm	显著性检验Significance test
合并基因型 Combined genotypes	样本量 N/头		GGGG	GGGA	GGAA	GCGG	GCGA	GCAA	CCGG	CCGA	CCAA
GGGG	41	12.32±2.03		0.186	0.000^**	0.167	0.007^**	0.000^**	0.144	0.037^*	0.002^**
GGGA	99	11.72±2.08	0.186		0.004^**	0.744	0.064	0.001^**	0.284	0.127	0.010^**
GGAA	68	10.61±2.97	0.000^**	0.004^**		0.048^*	0.340	0.498	0.770	0.777	0.161
GCGG	40	11.58±2.18	0.167	0.744	0.048^*		0.247	0.014^*	0.344	0.197	0.021^*
GCGA	68	11.01±2.58	0.007^**	0.064	0.340	0.247		0.119	0.569	0.471	0.070
GCAA	51	10.31±2.74	0.000^**	0.001^**	0.498	0.014^*	0.119		0.937	0.959	0.284
CCGG	3	10.20±0.74	0.144	0.284	0.770	0.344	0.569	0.937		0.922	0.577
CCGA	8	10.36±1.51	0.037^*	0.127	0.777	0.197	0.471	0.959	0.922		0.383
CCAA	7	9.26±1.90	0.002^**	0.010^**	0.161	0.021^*	0.070	0.284	0.577	0.383

Association Analysis of PTER and CALB2 Gene Polymorphisms with Growth Traits in American Yorkshire pigs

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