L-丙氨酸对公猪精液常温保存效果的影响

doi:10.16431/j.cnki.1671-7236.2026.02.032

Abstract

Abstract:

Objective L-alanine （L-Ala） is a multifunctional additive that functions both as a sweetener and a pH buffer. This study aimed to investigate the effect of L-Ala on the effect of boar semen storage at room temperature and provide a theoretical basis for optimizing the formulation of semen diluents. Method Semen was collected from three healthy adult Duroc boars aged 1 to 2 years. Various concentrations （0， 20， 40， 80， 160， and 320 μg/mL） of L-Ala were added to Modena diluent. Sperm motility and motion parameters （straight-line （rectilinear） velocity （VSL）， curvilinear velocity （VCL）， average path velocity （VAP）， and amplitude of lateral head （ALH）） were assessed at 17 ℃ on days 1， 3， 5， and 7 of storage. Samples showing significant changes were further analyzed for acrosome integrity， plasma membrane integrity， and oxidative stress indices. Result The effects of L-Ala on boar semen storage at room temperature were concentration- and time-dependent. During the first five days of storage， sperm motility parameters showed no significant differences in different concentration groups （0-320 μg/mL）（P>0.05）. By days 7， 320 μg/mL L-Ala group exhibited the best performance， among which sperm motility increased by 9.73% （P<0.05）， motility parameters （VSL， VAP， and ALH） increased by 11.33%， 13.08%， and 12.17% （P<0.05）， and acrosome integrity rate and plasma membrane integrity rate increased by 43.71% and 26.54% （P<0.05）， respectively， the total antioxidant capacity （T-AOC） increased by 1.14-fold （P<0.05）， while malondialdehyde （MDA） content decreased by 54.91% （P<0.05）. Conclusion Under the conditions of this experiment， L-Ala could effectively prolong the storage time of boar semen at room temperature， with 320 μg/mL being the optimal concentration. It significantly maintained sperm quality in the late storage period （7 days）， the mechanism of action might be related to enhancing antioxidant capacity and reducing lipid peroxidation damage.

Key words: L-Ala; boar semen; storage at room temperature; sperm motility

CLC Number:

S828.3

ZENG Guanghu, JIA Yuxuan, HUANG Mengting, SHEN Xiangyu, LIU Jiawei, DU Junhao, GONG Ting. Effect of L-alanine on Boar Semen Storage at Room Temperature[J]. China Animal Husbandry & Veterinary Medicine, 2026, 53(2): 870-878.

Figures/Tables 8

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References 25

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时间 Time/d	组别 Groups
时间 Time/d	对照 Control	Ⅰ	Ⅱ	Ⅲ	Ⅳ	Ⅴ
1	86.15±4.17^A	85.30±5.22^A	84.79±5.89^A	82.97±4.24^A	83.63±1.80^A	85.20±3.19^A
3	85.88±3.21^ABab	82.52±4.53^Ab	84.81±3.98^Aab	83.68±1.68^Aab	82.04±3.28^Aab	86.77±3.98^Aa
5	78.35±4.07^Bab	72.74±1.27^Bb	74.13±3.23^Bab	76.04±2.04^Bab	79.88±2.40^Ba	77.77±3.22^Bab
7	70.50±2.68^Cb	53.22±5.72^Cd	67.21±1.65^Bb	58.68±3.18^Cc	56.87±2.98^Ccd	77.36±0.98^Ba

时间 Time/d	组别 Groups
时间 Time/d	对照 Control	Ⅰ	Ⅱ	Ⅲ	Ⅳ	Ⅴ
1	67.81±0.78^Aab	65.86±4.42^Ab	70.26±0.73^Aa	68.87±3.50^Aab	67.78±0.32^Aab	67.29±1.75^ab
3	68.82±1.82^A	66.88±4.36^A	68.06±3.01^A	67.16±1.68^A	67.24±0.79^A	68.05±1.34
5	63.82±2.72^ABb	67.75±1.81^Aa	67.36±3.11^Aab	64.52±2.56^Aab	64.29±1.73^Aab	66.58±1.65^ab
7	58.58±3.49^Bb	50.72±3.09^Bc	58.73±1.32^Bb	56.11±2.05^Bb	54.42±3.64^Bbc	65.22±1.61^a

时间 Time/d	组别 Groups
时间 Time/d	对照 Control	Ⅰ	Ⅱ	Ⅲ	Ⅳ	Ⅴ
1	78.17±0.90^Aab	75.18±5.61^Ab	82.07±1.11^Aa	77.72±4.27^Aab	77.33±1.03^Aab	77.30±2.00^Aab
3	79.54±1.78^A	76.67±6.45^A	79.02±5.29^A	77.23±3.05^A	76.81±1.72^A	77.98±2.59^A
5	72.06±2.76^Bb	78.11±2.86^Aa	76.30±3.97^Aab	73.00±3.23^Ab	74.35±1.17^Aab	76.61±2.45^Aab
7	64.81±3.86^Cabc	55.67±3.92^Bd	66.09±2.42^Bab	61.36±2.46^Bbc	59.65±3.50^Bd	69.49±3.87^Ba

时间 Time/d	组别 Groups
时间 Time/d	对照 Control	Ⅰ	Ⅱ	Ⅲ	Ⅳ	Ⅴ
1	54.88±0.63^Aab	52.78±3.93^Ab	57.61±0.79^Aa	54.57±3.00^Aab	54.29±0.73^Aab	54.27±1.40^ab
3	55.85±1.24^A	53.82±4.53^A	55.48±3.71^A	54.22±2.14^A	53.92±1.21^A	54.75±1.82
5	50.59±1.93^Bb	54.84±2.00^Aa	53.57±2.78^Aab	51.25±2.27^Ab	52.20±0.82^Aab	53.79±1.72^ab
7	45.50±2.71^Cb	39.08±2.75^Bd	46.40±1.70^Bb	43.07±1.73^Bbc	41.88±2.46^Bcd	51.45±1.01^a

时间 Time/d	组别 Groups
时间 Time/d	对照 Control	Ⅰ	Ⅱ	Ⅲ	Ⅳ	Ⅴ
1	23.29±0.27^Aab	22.40±1.67^Ab	24.45±0.33^Aa	23.16±1.27^Aab	23.04±0.31^Aab	23.03±0.59^ABab
3	23.70±0.53^A	22.85±1.92^A	23.55±1.57^A	23.01±0.91^A	22.88±0.51^A	23.23±0.77^A
5	21.47±0.82^Ab	23.27±0.85^Aa	22.73±1.18^Aab	21.75±0.96^Ab	22.15±0.35^Aab	22.83±0.73^ABab
7	19.31±1.15^Bbc	16.59±1.17^Be	19.69±0.72^Bb	18.28±0.73^Bcd	17.78±1.04^Bde	21.66±0.22^Ba

Effect of L-alanine on Boar Semen Storage at Room Temperature

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