宣和猪ADRP和FASN基因多态性及其与肉质性状的关联分析

doi:10.16431/j.cnki.1671-7236.2026.02.033

Abstract

Abstract:

Objective This study aimed to screen single nucleotide polymorphism （SNP） of adipose differentiation-related protein （ADRP） and fatty acid synthase （FASN） genes in Xuanhe pigs， and reveal the association between SNP and meat quality traits in Xuanhe pigs. Method The meat quality traits in 120 Xuanhe pigs， including meat color， marbling， pH₁， drip loss， and intramuscular fat （IMF） content， were systematically measured. Furthermore， PCR amplification and Sanger sequencing were performed on ADRP and FASN genes， and the association between different genotypes at each SNP and meat quality traits in Xuanhe pigs was analyzed using a least squares model. Result Three SNPs were identified in exon 8 of ADRP gene， among which rs325276230 was synonymous mutation， and rs321564137 and rs81217193 were missense mutation， with dominant genotypes AA， AA， and GG， and dominant alleles A， A， and G， respectively. Three SNPs were in Hardy-Weinberg equilibrium （P>0.05）. Six SNPs were detected in FASN gene， including 4 synonymous mutation sites （rs337531100， rs324640280， rs81478148， and rs319161464） and 2 missense mutation sites （rs337267671 and rs342967881）. The dominant genotypes were TC， CC， TT， GG， GG， and CT， corresponding to dominant alleles C， C， T， G， G， and T， respectively. rs81478148 was deviated from Hardy-Weinberg equilibrium （P<0.01）. The association analysis results demonstrated that SNP of ADRP and FASN genes were significantly associated with meat traits in Xuanhe pigs. The IMF content， marbling， and pH₁of AA genotype at rs325276230 of ADRP gene were extremely significantly or significantly higher than those of GG genotype （P<0.01 or P<0.05）. The IMF content of AA genotype at rs321564137 was significantly higher than that of AG genotype， and the meat color of AA genotype was significantly lower than of AG genotype （P<0.05）. The IMF content of GG genotype at rs81217193 was significantly higher than that of GA and AA genotypes （P<0.05）. The IMF content of CC genotype at rs337531100 of FASN gene was significantly higher than that of TT and TC genotypes （P<0.05）. The meat color of TT genotype at rs324640280 was significantly higher than of CT genotype （P<0.05）. The meat color， pH₁， and drip loss of CT genotype at rs81478148 were extremely significantly or significantly higher than those of TT genotype （P<0.01 or P<0.05）. pH₁ of AA genotype at rs319161464 was significantly lower than that of GG and GA genotypes （P<0.05）. The marbling of GG genotype at rs337267671 was significantly higher than that of GA and AA genotypes （P<0.05）. The IMF content and marbling of TT genotype at rs342967881 were significantly higher than those of CC genotype （P<0.05）. Conclusion SNP of ADRP and FASN genes were significantly associated with meat quality traits in Xuanhe pigs， among which rs325276230， rs321564137， and rs81217193 of ADRP gene， and rs337531100 and rs342967881 of FASN gene could be used as potential molecular markers to improve the IMF content of Xuanhe pigs.

Key words: Xuanhe pigs; ADRP gene; FASN gene; single nucleotide polymorphism; meat quality trait

CLC Number:

S813.3

LAI Jinhua, WANG Xiaoyi, WU Ya, ZHU Dongxia, ZHU Yixuan, ZHANG Kezhong, HUANG Zhaojun, GUAN Heping, LI Mingli, LU Shaoxiong. Polymorphisms of ADRP and FASN Genes and Their Association with Meat Quality Traits in Xuanhe Pigs[J]. China Animal Husbandry & Veterinary Medicine, 2026, 53(2): 879-891.

Figures/Tables 10

Table 1

Table 2

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Table 3

Table 4

Table 5

Table 6

Association analysis between SNP of FASN gene and meat quality traits in Xuanhe pigs"

SNPs	基因型 Genotypes	IMF含量 IMF content/%	肉色 Meat color/分	大理石纹 Marbling/分	pH₁	滴水损失 Drip loss/%
rs337531100	TT	2.79±0.20^b	3.97±0.17	2.53±0.17	6.53±0.07	2.76±0.34
	TC	2.85±0.11^b	3.85±0.10	2.61±0.09	6.46±0.04	2.56±0.19
	CC	3.18±0.16^a	3.83±0.13	2.87±0.13	6.53±0.06	2.61±0.26
	a	0.33±0.19	―0.03±0.16	0.26±0.16	0.07±0.07	0.05±0.32
	d	―0.23±0.22	0.13±0.19	―0.21±0.19	0.03±0.08	0.18±0.38
rs324640280	CC	2.95±0.10	3.87±0.08^ab	2.63±0.08	6.48±0.04	2.60±0.17
	CT	2.81±0.18	3.79±0.14^b	2.73±0.14	6.55±0.06	2.57±0.28
	TT	3.37±0.63	4.75±0.54^a	3.00±0.53	6.25±0.23	3.21±1.05
	a	0.41±0.64	0.88±0.55	0.37±0.54	―0.23±0.24	0.60±1.07
	d	―0.35±0.37	―0.52±0.31	―0.09±0.30	0.19±0.13	―0.33±0.60
rs81478148	CC	2.71±0.32	3.94±0.26^ABab	2.13±0.26^b	6.71±0.12^a	2.19±0.52^b
	CT	2.92±0.21	4.43±0.17^Aa	2.89±0.17^a	6.52±0.08^a	3.30±0.35^a
	TT	2.95±0.10	3.75±0.08^Bb	2.67±0.08^a	6.47±0.04^b	2.51±0.16^b
	a	―0.03±0.23	0.68±0.19^*	0.22±0.19	0.05±0.08	0.79±0.39^*
	d	―0.23±0.34	―0.15±0.28	―0.65±0.28^*	0.21±0.12	―0.72±0.55
rs319161464	GG	2.96±0.10	3.89±0.08	2.65±0.08	6.49±0.04^a	2.63±0.17
	GA	2.83±0.18	3.74±0.14	2.69±0.14	6.55±0.06^a	2.52±0.28
	AA	3.00±0.52	4.48±0.44	2.84±0.43	6.10±0.19^b	2.74±0.86
	a	0.04±0.53	0.60±0.45	0.19±0.44	―0.38±0.19^*	0.11±0.88
	d	―0.14±0.32	―0.44±0.27	―0.05±0.26	0.25±0.11^*	―0.17±0.52
rs337267671	GG	2.99±0.12	3.82±0.10	2.84±0.09^a	6.49±0.04	2.59±0.19
	GA	2.90±0.13	3.90±0.11	2.50±0.10^b	6.47±0.05	2.62±0.21
	AA	2.42±0.40	4.14±0.35	2.09±0.33^b	6.72±0.15	2.67±0.75
	a	―0.57±0.42	0.32±0.36	―0.75±0.34^*	0.22±0.15	0.08±0.77
	d	0.20±0.24	―0.09±0.21	0.04±0.20	―0.14±0.09	―0.01±0.44
rs342967881	CC	2.64±0.21^b	4.13±0.18	2.42±0.17^b	6.56±0.08	2.87±0.36
	CT	2.93±0.12^ab	3.85±0.10	2.65±0.09^a	6.47±0.04	2.54±0.19
	TT	3.09±0.16^a	3.75±0.13	2.84±0.13^a	6.49±0.06	2.59±0.26
	a	―0.44±0.26	0.38±0.22	―0.42±0.22^*	0.07±0.10	0.28±0.45
	d	0.07±0.17	―0.09±0.15	0.02±0.14	―0.06±0.06	―0.19±0.29

Table 6

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引物 Primers	引物序列 Primer sequences （5'→3'）	位置 Location	产物大小 Size of product/bp
ADRP	F： AGGGTTGTTTGTGAGGATTTT R： GCTAGGCTCTGACCAGTCTGT	内含子8、外显子8	763
FASN-1	F： CTGGCCTCAGGCAGAGGGTCTG R： TTGTGAAATGTGACCTGCCCGAG	内含子2、外显子3	554
FASN-2	F： TCCTTGTGACCTGTTTTCCGTTCC R： CGGCGGGAGGTTGTCAGGAAG	内含子3、外显子4、内含子5	421
FASN-3	F： CGGACAGCAGCCGAGACCCTT R： ACGGCCTCCGCACGGCAATAG	外显子5、内含子、外显子6	500
FASN-4	F： GGGGTAATGGTAGGCGTGAGGG R： ACAGGGAAGAGGGGCTGGGAC	外显子21、内含子22、外显子22	657

引物 Primers	预变性 Pre-denaturation	变性 Denaturation	退火 Annealing/℃	延伸 Extension	循环 Cycles/个	再延伸 Extension
ADRP	94 ℃ 5 min	94 ℃ 40 s	61	72 ℃ 40 s	35	72 ℃ 7 min
FASN-1	94 ℃ 5 min	94 ℃ 35 s	69	72 ℃ 35 s	35	72 ℃ 7 min
FASN-2	94 ℃ 5 min	94 ℃ 35 s	69	72 ℃ 35 s	35	72 ℃ 7 min
FASN-3	94 ℃ 5 min	94 ℃ 35 s	65	72 ℃ 35 s	35	72 ℃ 7 min
FASN-4	94 ℃ 5 min	94 ℃ 42 s	68	72 ℃ 42 s	35	72 ℃ 7 min

SNPs	基因型 Genotypes	IMF含量 IMF content/%	肉色 Meat color/分	大理石纹 Marbling/分	pH₁	滴水损失 Drip loss/%
rs325276230	AA	3.06±0.10^Aa	3.84±0.08	2.73±0.08^a	6.50±0.04^Aa	2.60±0.16
	AG	2.58±0.16^Bb	4.01±0.14	2.53±0.13^a	6.51±0.06^Aa	2.68±0.27
	GG	2.42±0.62^Bb	3.00±0.54	1.75±0.52^b	5.80±0.23^Bb	1.97±1.05
	a	―0.64±0.62	―0.84±0.54	―0.98±0.53^*	―0.70±0.23^**	―0.63±1.06
	d	―0.16±0.35	0.59±0.30	0.29±0.29	0.35±0.13^**	0.39±0.60
rs321564137	AA	3.00±0.09^a	3.81±0.08^b	2.69±0.08	6.49±0.03	2.64±0.15
	AG	2.38±0.23^b	4.27±0.20^a	2.49±0.20	6.49±0.09	2.39±0.39
	a	/	/	/	/	/
	d	/	/	/	/	/
rs81217193	GG	3.04±0.10^a	3.84±0.09	2.69±0.08	6.50±0.04	2.56±0.17
	GA	2.55±0.17^b	4.00±0.15	2.60±0.14	6.49±0.06	2.82±0.29
	AA	2.99±0.29^b	3.75±0.26	2.55±0.25	6.37±0.11	2.39±0.50
	a	―0.05±0.31	―0.09±0.27	―0.15±0.26	―0.13±0.12	―0.18±0.52
	d	―0.47±0.23^*	0.21±0.20	―0.02±0.19	0.06±0.08	0.35±0.38

Polymorphisms of ADRP and FASN Genes and Their Association with Meat Quality Traits in Xuanhe Pigs

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[4]	JIA Qianru, LUO Xuan, TIAN Tian, WANG Lei, MA Yuhong, SHANG Yuejun, XU Fafang, MO Huashan, WU Guofang. Effects of Crossbreeding on Meat Quality and Regulatory Mechanism of Local Pig Breeds and Introduced Pig Breeds [J]. China Animal Husbandry & Veterinary Medicine, 2025, 52(12): 5762-5771.
[5]	JIA Yuxuan, HAN Fei, ZENG Guanghu, SHEN Xiangyu, GONG Ting. Polymorphism of TAS1R1 Gene and Its Association with Growth Traits in Guizhou Indigenous Pigs [J]. China Animal Husbandry & Veterinary Medicine, 2025, 52(12): 5809-5820.
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[10]	XIAO Wen, HAN Yong, YANG Hongwen, LONG Yong, SU Chaozhi, YUAN Chao, YANG Yang, WANG Defeng, ZHAO Yanpin, QIN Yang, YIN Fuyue. Comparative Study on Fattening Performance,Slaughter Performance and Meat Quality of Black Goats and Their Hybrid Offspring [J]. China Animal Husbandry & Veterinary Medicine, 2024, 51(6): 2429-2439.
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[15]	YANG Man, LIU Hai, ZHANG Run, HU Ziping, NIU Naiqi, WANG Lixian, ZHANG Longchao. Association Analysis of Polymorphism of MYH3 and MYH13 Genes with Meat Quality Traits in Beijing Black Pigs [J]. China Animal Husbandry & Veterinary Medicine, 2022, 49(9): 3428-3437.