关岭黄牛GH1基因多态性及其与生长性状的关联分析和GH1蛋白功能预测

doi:10.16431/j.cnki.1671-7236.2025.09.021

Abstract

Abstract: 【Objective】 Growth hormone 1 (GH1) is a synthetic metabolic hormone belonging to the growth hormone/prolactin protein family,which plays an extremely important role in animal growth and development.This study was aimed to screen the single nucleotide polymorphism (SNP) of GH1 gene and conduct association analysis with growth traits in Guanling cattle,in order to provide scientific basis for molecular breeding of Guanling cattle. 【Method】 Using Primer Premier 3.0 software,three pairs of primers were designed based on the bovine GH1 gene sequence retrieved from the NCBI database (accession No.:NC_037346.1) to amplify GH1 gene in Guanling cattle.SNP were detected via bidirectional direct sequencing and sequence alignment.HaploView software was employed to perform linkage disequilibrium (LD) analysis on the identified SNP and identify associated haplotypes,thereby elucidating the genetic interactions and structural relationships among these loci.The correlation between GH1 gene and growth traits in Guanling cattle was evaluated using SPSS 21.0 software,and the potential function of GH1 protein was predicted. 【Result】 Three SNPs were detected in exon 5 of GH1 gene in Guanling cattle:g.44119526 C＞G,g.44119662 C＞T,and g.44119676 C＞A.g.44119526 C＞G and g.44119676 C＞A showed moderate polymorphism,while g.44119662 C＞T exhibited low polymorphism.g.44119662 C＞T and g.44119676 C＞A were in Hardy-Weinberg equilibrium (P＞0.05),while g.44119526 C＞G was extremely significantly deviated from Hardy-Weinberg equilibrium (P＜0.01).Three SNPs formed four haplotypes (H1,H2,H3,and H4) and five diplotypes (H1H1,H1H2,H1H3,H1H4,and H2H3).The association analysis results revealed that the back waist length of CC genotype of g.44119676 C＞A in GH1 gene of Guanling cattle was significantly higher than that of AA genotype (P＜0.05),and the back waist length of H1H1(CCCCCC) diplotype was significantly higher than that of H2H3(GCCCAA) diplotype (P＜0.05).The protein function prediction results showed that GH1 gene encoded 217 amino acids and was a stable protein.The secondary and tertiary structures were mainly composed of alpha helix and random coil. 【Conclusion】 The mutation of g.44119676 C＞A on exon 5 of GH1 gene in Guanling cattle had a significant impact on the back waist length.The diplotype H1H1 could be used as a genetic marker to improve the growth trait of Guanling cattle.

Key words: Guanling cattle; GH1 gene; growth traits; single nucleotide polymorphism; protein function

CLC Number:

S813.3

ZHANG Haijuan, ZHAO Yong, YANG Hongwen, WU Yan, XIANG Jin, FU Hongmei, DING Yijie, ZHANG Yiyu. Polymorphism of GH1 Gene and Its Association with Growth Traits and Functional Prediction of GH1 Protein in Guanling Cattle[J]. China Animal Husbandry & Veterinary Medicine, 2025, 52(9): 4226-4236.

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Polymorphism of GH1 Gene and Its Association with Growth Traits and Functional Prediction of GH1 Protein in Guanling Cattle

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