绵羊MyoG基因内含子Ⅱ多态性及其与生长性状的关联分析

doi:10.16431/j.cnki.1671-7236.2022.04.018

Abstract

Abstract: 【Objective】 This study was aimed to analyze the polymorphism of myogenin (MyoG) gene intron Ⅱ and its effect on growth traits in sheep,the molecular markers with significant effects on the growth and development of sheep were screened to provide reference for molecular breeding in sheep.【Method】 Five species of Large-tailed Han sheep,Small-tailed Han sheep,Yuxi Fat-tailed sheep,Dorper sheep and Hu sheep were selected as the research object. The intron Ⅱ of MyoG gene (Eco72 Ⅰ) was genotyped by PCR-RFLP technology.The population genetic polymorphism of MyoG gene intron Ⅱ was calculated by PopGene32 software.The association analysis between the different genotypes of MyoG gene intron Ⅱ and growth traits of sheep was performed by SPSS 17.0 software.【Result】 There were three genotypes of AA (368/540 bp),AB (908/368/540 bp) and BB (908 bp) were found of MyoG gene intron Ⅱ in Small-tailed Han sheep,Dorper sheep and Hu sheep populations,and there were two genotypes of AB (908/368/540 bp) and BB (908 bp) were found in Large-tailed Han sheep and Yuxi Fat-tailed sheep populations.The genotype frequency of AB in Large-tailed Han sheep,Small-tailed Han sheep,Yuxi Fat-tailed sheep,Hu sheep and Dorper sheep were 0.845,0.633,0.917,0.706 and 0.811,the genotype frequency of BB were 0.155,0.033,0.083,0.176 and 0.054,and the genotype frequency of AA genotype were 0,0.333,0,0.118 and 0.135,respectively.The lowest heterozygosity was 0.455 in Small-tailed Han sheep,and the highest was 0.497 in Dorper sheep,indicating that Dorper sheep had a higher genetic diversity than other population.The polymorphic information content (PIC) of five sheep population was moderate polymorphism (0.25<PIC<0.5).The association analysis results showed that the chest circumference,chest width,head length and neck length of BB genotype of MyoG gene intron Ⅱ in Small-tailed Han sheep were significantly lower than those of AA and AB genotypes (P<0.05).【Conclusion】 The Eco72 Ⅰ site of MyoG gene intron Ⅱ could be used as a molecular marker affecting the growth trait in sheep,and the results provided reference for molecular marker assisted selection of growth trait in sheep in the future.

Key words: sheep; MyoG gene; intron Ⅱ; Eco72 Ⅰ site; growth traits

CLC Number:

S813.3

LI Jingyun, WANG Xinle, ZHANG Yunfei, BAI Junyan, LI Gan, LEI Ying, DONG Zhihao, CHEN Yu, FAN Hongdeng, WANG Longwei, CHEN Mengke, WANG Xingke, SIMA Leiyang, ZHANG Shiwen. Polymorphism of MyoG Gene Intron Ⅱ and Its Association with Growth Traits in Sheep[J]. China Animal Husbandry & Veterinary Medicine, 2022, 49(4): 1364-1373.

References

[1] DAVEGRDH C,BROHOLM C,PERFILYEV A,et al.Abnormal epigenetic changes during differentiation of human skeletal muscle stem cells from obese subjects[J].BMC Medicine,2017,15(1):39.
[2] LIU Z,ZHANG X,LEI H,et al.CASZ1 induces skeletal muscle and rhabdomyosarcoma differentiation through a feed-forward loop with MyoD and MyoG[J].Nature Communications,2020,11(1):911.
[3] 周国利,朱奇,吴玉厚,等.Myogenin基因的分子生物学综述[J].生物技术,2005,15(1):90-91. ZHOU G L,ZHU Q,WU Y H,et al.Molecular biology of Myogenin gene:A review[J].Biotechnology,2005,15(1):90-91.(in Chinese)
[4] BERTI F,MEIRELES N J,WOHRLE S,et al.Time course and side-by-side analysis of mesoderma1,pre-myogenic,myogenic and differentiated cell markers in the chicken model for skeletal muscle formation[J].Journal of Anatomy,2015,227(3):361-382.
[5] ZHANG Z T,XU F,ZHANG Y N,et al.Cloning and expression of MyoG gene from Hu sheep and identification of its myogenic specificity[J].Molecular Biology Reports,2014,41(2):1003-1013.
[6] ZHANG G X,TANG Y,ZHANG T,et al.Expression profiles and association analysis with growth traits of the MyoG and Myf5 genes in the Jinghai Yellow chicken[J].Molecular Biology Reports,2014,41(11):7331-7338.
[7] ZHAO X H,LI M Y,XU S S,et al.Expression of myostatin (MSTN) and myogenin (MyoG) genes in Zi and Rhine goose and their correlation with carcass traits[J].Brazilian Journal of Poultry Science,2019,21(1):1-6.
[8] SUN W,SU R,LI D,et al.Developmental changes in IGF-Ⅰ and MyoG gene expression and their association with meat traits in sheep[J].Genetics and Molecular Research,2014,13(2):2772-2783.
[9] TEPAS M F W,SOUMILLION A,RETTENBERGER G,et al.Characterization of the porcine myogenin gene locus and association between polymorphism and growth traits[J].Animal Genetis,1996,27(Suppl.2):117.
[10] 王健,董飚,侯庆永,等.太湖鹅MyoG基因多态性与体重的相关性分析[J].浙江农业学报,2015,27(1):28-31. WANG J,DONG B,HOU Q Y,et al.Association analysis between polymorphism of MyoG gene and body weight in Taihu goose[J].Acta Agriculturae Zhejiangensis,2015,27(1):28-31.(in Chinese)
[11] 郭琳,柴志欣,钟金城,等.三江黄牛DKK1、DKK4和MyoG基因遗传多态性分析[J].家畜生态学报,2018,39(6):16-20. GUO L,CHAI Z X,ZHONG J C,et al.Genetic diversity of DKK1,DKK4 and MyoG genes in Sanjiang cattle[J].Acta Ecologiae Animalis Domastici,2018,39(6):16-20.(in Chinese)
[12] 王琼,刘益平,蒋小松,等. MyoG基因多态性与优质肉鸡屠宰性状和肉质性状的相关性分析[J]. 遗传,2007,29(9):1089-1096. WANG Q,LIU Y P,JIANG X S,et al.Correlation analysis of relationships between polymorphisms of high quality chicken myogenin gene and slaughter and meat quality traits[J].Hereditas,2007,29(9):1089-1096.(in Chinese)
[13] 柴志欣,信金伟,王会,等. MyoG基因多态性与牦牛生长性能的关联分析[J].华北农学报,2018,33(6):108-115. CHAI Z X,XIN J W,WANG H,et al.Polymorphisms of MyoG gene and analysis of relevance with growth performances in yak[J].Acta Agriculturae Boreali-Sinica,2018,33(6):108-115.(in Chinese)
[14] 彭邦星,李辉,易恒洁,等. 兴义鸭MyoG基因SNPs与屠宰性状相关性分析[J].黑龙江畜牧兽医,2017,3:79-83. PENG B X,LI H,YI H J,et al.Correlation analysis between the SNPs of MyoG gene and slaughter traits in Xingyi ducks[J].Heilongjiang Animal Science and Veterinary Medicine,2017,3:79-83.(in Chinese)
[15] 张沅.家畜育种学[M]. 北京:中国农业出版社,2001. ZHANG Y.Animal Breeding[M].Beijing:China Agriculture Press,2001.(in Chinese)
[16] 刘铮铸,巩元芳,付志新,等. 山羊MyoG基因内含子Ⅱ变异及其在体质量上的遗传效应分析[J].畜牧兽医学报,2011,42(7):1015-1021. LIU Z Z,GONG Y F,FU Z X,et al.Genetic variation of goat MyoG gene intron Ⅱ and its genetic effects on body weight[J].Acta Veterinaria et Zootechnica Sinica,2011,42(7):1015-1021.(in Chinese)
[17] 赵祎,王思寒,刘磊,等. 延边黄牛UCP2、UCP3基因多态性及其与生长性状的关联分析[J].中国畜牧兽医,2021,48(10):3691-3698. ZHAO Y,WANG S H,LIU L,et al.Polymorphism of UCP2 and UCP3 genes and its association with growth traits in Yanbian Yellow cattle[J].China Animal Husbandry & Veterinary Medicine,2021,48(10):3691-3698.(in Chinese)
[18] 赵志达,张莉.基因组选择在绵羊育种中的应用[J].遗传,2019,41(4):293-303. ZHAO Z D,ZHANG L.Applications of genome selection in sheep breeding[J].Hereditas,2019,41(4):293-303.(in Chinese)
[19] 白俊艳,曹恒,王旭,等. 绵羊GH基因的Pvu Ⅱ位点的多态性与其生长性状的关联分析[J].浙江农业学报,2019,31(9):1416-1422. BAI J Y,CAO H,WANG X,et al.Association between the polymorphism of Pvu Ⅱ locus of GH gene and growth traits in sheep[J].Acta Agriculturae Zhejiangensis,2019,31(9):1416-1422.(in Chinese)
[20] 肖超能,翁吉梅,李维,等. 家兔IGF1基因多态性与生长性状的关联分析[J].中国畜牧兽医,2017,44(8):2378-2385. XIAO C N,WONG J M,LI W,et al.Association analysis between polymorphism of IGF1 gene and growth traits in rabbit[J].China Animal Husbandry & Veterinary Medicine,2017,44(8):2378-2385.(in Chinese)
[21] 董智豪,陈宇,黄高想,等.蛋用鹌鹑的VIPR-1基因的多态性与早期生长性状的关联分析[J].浙江农业学报,2021,33(8):1393-1401. DONG Z H,CHEN Y,HUANG G X.Association analysis of VIPR-1 gene polymorphism and early growth traits in egg quail[J].Acta Agriculturae Zhejiangensis,2021,33(8):1393-1401.(in Chinese)
[22] 海汀,柴志欣,钟金城.麦洼牦牛GH、GHR、GHSR基因的SNPs检测及其与体尺性状的关联分析[J].畜牧兽医学报,2017,48(4):605-617. HAI T,CHAI Z X,ZHONG J C.Identification of SNPs in GH,GHR and GHSR genes and their association with body measurement traits in Maiwa yak[J].Acta Veterinaria et Zootechnica Sinica,2017,48(4):605-617.(in Chinese)
[23] 宋兴超,徐超,刘琳玲,等.吉林梅花鹿肌肉发育关联基因MyoG的鉴定及生物信息学分析[J].特产研究,2019,41(4):11-17. SONG X C,XU C,LIU L L,et al.Identification and bioinformatics analysis of MyoG gene related to muscle development in Jilin sika deer (Cervus nippon)[J].Special Wild Economic Animal and Plant Research,2019,41(4):11-17.(in Chinese)
[24] 刘永斌,王峰,邓凤,等.绵羊MyoG基因外显子的单核苷酸多态性群体遗传学分析[J].畜牧兽医学报,2007,38(12):1294-1299. LIU Y B,WANG F,DENG F,et al.Analysis on single nucleotide polymorphisms of exon of MyoG gene in different sheep breeds[J].Acta Veterinaria et Zootechnica Sinica,2007,38(12):1294-1299.(in Chinese)
[25] 孙奴奴,刘瑾.猪MyoG基因外显子1的多态性分析[J].运城学院学报,2020,38(3):5-8. SUN N N,LIU J.Polymorphism analysis of exon 1 of porcine MyoG gene[J].Journal of Yuncheng University,2020,38(3):5-8.(in Chinese)
[26] 魏天,马惠海,鲍志鸿,等.小尾寒羊MyoG基因外显子1的克隆及多态性分析[J].黑龙江农业科学,2019,12:15-17. WEI T,MA H H,BAO Z H,et al.Cloning and polymorphism analysis of MyoG gene exon 1 in Small Tailed Han sheep[J].Heilongjiang Agricultural Sciences,2019,12:15-17.(in Chinese)
[27] 白俊艳,杨又兵,王玉琴,等.绵羊MyoG基因外显子1的多态性及其与肉质性状的关联分析[J].中国畜牧兽医,2017,44(6):1778-1783. BAI J Y,YANG Y B,WANG Y Q,et al.Polymorphism of MyoG gene exon 1 and its association analysis with meat quality traits in sheep[J].China Animal Husbandry & Veterinary Medicine,2017,44(6):1778-1783.(in Chinese)
[28] 姚毅,马红艳,吕学斌,等. MyoG基因多态性与山羊生长性能的相关性分析[J].山西农业科学,2015,43(11):1496-1499. YAO Y,MA H Y,LYU X B,et al.Polymorphisms of MyoG gene and analysis of relevance with growth performances in goats[J].Journal of Shanxi Agricultural Sciences,2015,43(11):1496-1499.(in Chinese)
[29] 刘铮铸,巩元芳,张传生,等. 波尔山羊MyoG基因的鉴定和序列分析[J].畜牧兽医学报,2010,41(10):1337-1341. LIU Z Z,GONG Y F,ZHANG C S,et al.Identification and sequence analysis of the MyoG gene in Boer goat(Capra hircus)[J].Acta Veterinaria et Zootechnica Sinica,2010,41(10):1337-1341.(in Chinese)
[30] 韩银仓,孙永刚,陈志. MyoG基因SNP检测及与藏羊体尺性状的关联性分析[J]. 家畜生态学报,2016,37(6):19-23. HAN Y C,SUN Y G,CHEN Z.Single nucleotide polymorphism of MyoG gene in Tibetan sheep populationas and its association with body measurement traits[J].Journal of Domestic Animal Ecology,2016,37(6):19-23.(in Chinese)
[31] 储明星,何远清,王金玉,等. 绵羊肌细胞生成素基因外显子1的PCR-SSCP分析[J].农业生物技术学报,2005,13(1):77-80. CHU M X,HE Y Q,WANG J Y,et al. PCR-SSCP analysis on exon 1 of myogenin gene in sheep[J].Journal of Agricultural Biotechnology,2005,13(1):77-80.(in Chinese)
[32] 王旭,白俊艳,杨帅,等.家畜肌细胞生成素(MyoG)基因的研究进展[J].安徽农学通报,2016,22(20):76-77. WANG X,BAI J Y,YANG S,et al.Research progress of myogenin (MyoG) gene in livestock[J].Anhui Agricultural Science Bulletin,2016,22(20):76-77.(in Chinese)
[33] 郭云雁,程笃学,张龙超,等. MyoG和c-fos基因多态性及其合并基因型与猪肌纤维性状的关联分析[J].畜牧兽医学报,2013,44(2):188-196. GUO Y Y,CHENG D X,ZHANG L C,et al.Effects of single and combined genotypes of MyoG and c-fos genes on muscle fiber traits in pig[J].Acta Veterinaria et Zootecinica Sinica,2013,44(2):188-196.(in Chinese)
[34] 朱超杰,张立春,秦立红,等.不同品种牛MyoG基因Exon 1多态性比较分析[J].中国兽医学报,2016,36(6):1024-1027. ZHU C J,ZHANG L C,QIN L H,et al.Polymorphism analysis on exon 1 of MyoG gene in different cattle population[J].Chinese Journal of Veterinary Science,2016,36(6):1024-1027.(in Chinese)
[35] 翁吉梅.黔北麻羊FTO基因多态性及其表达与肉质性状的关联性研究[D].贵州:贵州大学,2019. WENG J M.Association study for polymorphism and expression of FTO gene with meat quality traits in Qianbei Brown goat[D].Guizhou:Guizhou University,2019.(in Chinese)
[36] 王彦钦.功能基因多态性对拜城油鸡生产性能影响的研究[D].乌鲁木齐:新疆农业大学,2016. WANG Y Q.Study on the effect of functional gene polymorphism on production performance of Baicheng Fatty chicken[D].Urumqi:Xinjiang Agricultural University,2016.(in Chinese)
[37] 李云霞,姚新奎,孟军,等. 伊犁马MyoG基因外显子1多态性与肉质性状及肌纤维性状关联分析[J].中国畜牧兽医,2021,48(7):2504-2511. LI Y X,YAO X K,MENG J,et al.Correlation analysis between polymorphism of MyoG gene exon 1 with meat quality traits and muscle fiber traits in Yili horse[J].China Animal Husbandry & Veterinary Medicine,2021,48(7):2504-2511.(in Chinese)
[38] 韦宏伟.山羊Myf5、MyoG基因多态性及其与生长性状的关联性分析[D].雅安:四川农业大学,2010. WEI H W.Association of ploymorphisms of Myf5 and MyoG genes with growth performance in goats[D].Ya’an:Sichuan Agricultural University,2010.(in Chinese)
[39] BAI J Y,SHI K P,LU X N,et al.Association between myogenin gene polymorphism and slaughter traits of meat quails[J].Pakistan Journal of Zoology,2021,53(2):789-792.
[40] 孙伟,王鹏,丁家桐,等. 湖羊Myostain和Myogenin基因表达的发育性变化及与屠宰性状的关联分析[J].中国农业科学,2010,43(24):5129-5136. SUN W,WANG P,DING J T,et al.Developmental changes of gene expression of Myostain and Myogenin genes and their association analysis with carcass traits in Hu sheep[J].Scientia Agricultura Sinica,2010,43(24):5129-5136.(in Chinese)
[41] 白俊艳,时坤鹏,卢小宁,等.鹌鹑MyoG基因多态性与早期生长性能的关联分析[J].河南农业科学,2020,49(4):147-152. BAI J Y,SHI K P,LU X N,et al.Association analysis between MyoG gene polymorphism and early growth performance of quail[J].Journal of Henan Agricultural Sciences,2020,49(4):147-152.(in Chinese)
[42] HUANG Y,LAI X,HU L,et al.Over-expression of DEC1 inhibits myogenic differentiation by modulating MyoG activity in bovine satellite cell[J].Journal of Cellular Physiology,2018,233(12):9365-9374.

Polymorphism of MyoG Gene Intron Ⅱ and Its Association with Growth Traits in Sheep

PDF (PC)

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 15

Recommended Articles

Metrics

Comments

[1]	LI Wei, HUANG Qiaoyan, WANG Xinkun, GU Ruohuai, SUN Huiping, ZHU Lexiao, XING Feng. Cloning, Sequence Analysis and Protein Expression of LIN28A Gene Promoter in Duolang Sheep [J]. China Animal Husbandry & Veterinary Medicine, 2025, 52(7): 2992-3003.
[2]	CAI Wenjing, CI Qiuyang, TANG Lin, LIU Hang, SUN Xinming, CHEN Yang, JIANG Huaizhi. Differential Expression Analysis of FABP4 Gene in Mammary Gland of Small-tailed Han Sheep Before and After Pregnancy [J]. China Animal Husbandry & Veterinary Medicine, 2025, 52(7): 3234-3241.
[3]	NIU Shuran, PAN Jianfeng, RONG Youjun, AO Xiaofang, WANG Yihan, SHANG Fangzheng, WANG Ruijun, ZHANG Yanjun. Advances on the Application of Circular RNA in Important Economic Traits in Sheep [J]. China Animal Husbandry & Veterinary Medicine, 2025, 52(6): 2468-2481.
[4]	ZHOU Lusong, ZHAO Yuanyuan, YANG Yuwei, ZHAO Qingyu, MA Qing, TANG Chaohua, ZHANG Huiyan, ZHANG Junmin, QIN Yang, QIN Yuchang. Effects of Sophora alopecuroides and Alkaloids from Sophora alopecuroides on Growth Performance and Rumen Microbiota of Tan Sheep [J]. China Animal Husbandry & Veterinary Medicine, 2025, 52(6): 2540-2551.
[5]	ZHANG Chengrui, WEI Xuesheng, ZHANG Mingzhu, WANG Jie, FAN Dingkun, ZHANG Juan, ZHU Dezhi, GUO Fusuo, ZHANG Naifeng. Protein Requirements of Growing Hu Sheep Ewe Lambs During 3 to 5 Months of Age [J]. China Animal Husbandry & Veterinary Medicine, 2025, 52(5): 2128-2139.
[6]	SHAN Mingzhu, ZHOU Lisheng, NAOMING Gaowa, ZHANG Xiaoxu, XU Yan, CHU Mingxing, PAN Zhangyuan. The Classification Study of Ovine Horn Type [J]. China Animal Husbandry & Veterinary Medicine, 2025, 52(5): 2177-2186.
[7]	WURI Lige, LI Na, GERILE Gelaba, HASI Tuya, ZHANG Jingjing, HOU Bin, HASI Surong. Molecular Identification of the Pathogen of Paramphistomiasis in Ordos Fine-wool Sheep and Screening of Deworming Drugs [J]. China Animal Husbandry & Veterinary Medicine, 2025, 52(4): 1807-1814.
[8]	DU Jiawen, REN Wenyi, XU Xiaofeng, ZHANG Lili. Comparison of the Transcriptome of Longissimus Dorsi Muscle of Tan Sheep at Different Growth Stages [J]. China Animal Husbandry & Veterinary Medicine, 2025, 52(3): 1011-1021.
[9]	ZHOU Na, LI Caishan, ZHAO Xueqing, ABUDUKADIER· Mejiti, DENG Yuqian, LIU Shiyu, SHI Wenyu, GUO Qingyong, BAYINGCHAHAN· Gailike. PCR Detection and Genetic Diversity Analysis of Theileria of Sheep in Aksu,Xinjiang [J]. China Animal Husbandry & Veterinary Medicine, 2025, 52(3): 1292-1300.
[10]	LI Na, LIU Chongyang, ZHANG Jingjing, MALIYA Qiqige, ZHU Na, LU Bin, HAI Ying. Isolation,Identification and Drug Resistance Analysis of a Sheep-derived Strain of Clostridium perfringens Type D [J]. China Animal Husbandry & Veterinary Medicine, 2025, 52(3): 1352-1359.
[11]	FENG Zhiya, PENG Wanwan, ZHANG Jianping, WU Zhenhui, ZHANG Nan, LI Shuwei, SHI Ruijun. Cloning and Bioinformatics Analysis of KRT79 Gene,and the Effects of Androgens on Its Expression and Distribution of Skin in Hetian Sheep [J]. China Animal Husbandry & Veterinary Medicine, 2025, 52(2): 562-573.
[12]	LIU Shixiong, LI Xiao, LI Xueqiang, XU Zixuan, LI Songjian, DU Rui, XUE Chen, LIU Dacheng. Effects of Compound Bacteria Culture on Growth Performance,Immune Function and Vaccine Antibody Titer of Mongolian-Han Crossbred Mutton Sheep [J]. China Animal Husbandry & Veterinary Medicine, 2025, 52(2): 718-729.
[13]	HE Xiaolong, SHEN Qi, BAO Guosheng, WANG Jiahao, JIANG Ting, YI Huanming, CHENG Xiao, CHEN Jiahong, REN Chunhuan, ZHANG Zijun, WANG Qiangjun. Research Progress on the Fermentation Effect of Microbial Agents on Sheep Manure Compost [J]. China Animal Husbandry & Veterinary Medicine, 2025, 52(2): 966-976.
[14]	XIE Beiyiting, WANG Yue, MENG Chunhua, QIAN Yong, ZHANG Jun, ZHANG Jianli, WANG Huili, CAO Shaoxian, LI Yinxia. Cloning and Sequence Analysis of PSMB9 Gene in Hu Sheep and Its Effect on Myoblast Proliferation [J]. China Animal Husbandry & Veterinary Medicine, 2025, 52(1): 1-12.
[15]	YU Tianli, HE Zhenlian, HAN Yijing, REN Jingyu, XIA Chengqiang, PEI Caixia. Effects of Adding Polygonum hydropiper L.to High-concentrate Diet on Fermentation Parameters and Flora of Rumen in Sheep [J]. China Animal Husbandry & Veterinary Medicine, 2025, 52(1): 205-214.