基于简化基因组测序的吐鲁番黑羊遗传结构分析

doi:10.16431/j.cnki.1671-7236.2025.09.020

Abstract

Abstract: 【Objective】 This study was aimed to investigate the population genetic structure and relatedness of breeding rams in the core group of Turpan Black sheep,evaluate their genetic diversity,and provide scientific data support for formulating rational breeding strategies and conservation plans. 【Method】 Single nucleotide polymorphism (SNPs) of 128 Turpan Black sheep rams were detected using simplified genome sequencing technology.Genetic diversity parameters and runs of homozygosity (ROH) were calculated using PLINK 1.90 and R 4.4.1 software.Population structure was analyzed through phylogenetic tree construction with Mega 10.0 software. 【Result】 The average observed heterozygosity (Ho),expected heterozygosity (He) and minor allele frequency (MAF) were 0.16,0.18 and 0.23,respectively,with a polymorphic information content (PIC) of 0.82.The 128 rams were clustered into 5 subpopulations based on principal component analysis (PCA) and phylogenetic analysis.The genetic distance (D-value) ranged from 0.1626 to 0.2749,with an average of 0.2635.The genetic distance and phylogenetic G matrix were consistent,indicating that the majority of individuals within the Turpan Black sheep population had distant phylogenetic relationships.A total of 2 239 ROHs were detected,of which 61.37% were 0-5 Mb in length.The average inbreeding coefficient (F_ROH) was 0.04,suggesting low inbreeding levels. 【Conclusion】 The breeding core group of Turpan Black sheep demonstrated high genetic diversity,with distant kinship among most individuals and low inbreeding levels within the population,indicating a robust foundation for genetic resource conservation and utilization.The results provided a scientific reference for developing breeding programs and sustainable utilization strategies for the Turpan Black sheep core population.

Key words: Turpan Black sheep; population genetic structure; kinship; runs of homozygosity

CLC Number:

S813.3

LUO Chunyan, BAI Feng, TENG Wen, AMINIGULI·Abulizi, MAERZIYA·Yasen, ZHOU Xirong, NAZAKAITI·Ainiwaner, MAISITUERGULI·Abulitipu, ZHANG Yuntao, JI Xinmin, ZHANG Yanhua. Genetic Structure Analysis of Turpan Black Sheep Based on Simplified Genome Sequencing[J]. China Animal Husbandry & Veterinary Medicine, 2025, 52(9): 4216-4225.

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Genetic Structure Analysis of Turpan Black Sheep Based on Simplified Genome Sequencing

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