连翘酯苷A通过HIF-1α调控牦牛卵丘细胞雌激素合成与分泌

doi:10.16431/j.cnki.1671-7236.2025.09.019

Abstract

Abstract: 【Objective】 This study was aimed to investigate the effects of forsythiaside A (FA) on estrogen synthase in cumulus cells of healthy adult female yak (5-8 years old) and explore its mechanism,so as to provide a scientific basis for FA application in yak reproductive management. 【Method】 Yak cumulus cells during the logarithmic growth phase were used for cytotoxicity assays with concentration gradients (0,5,10,20,30,and 40 μmol/L) and time gradients (12,24,36,and 48 h) to determine optimal FA treatment conditions.Real-time quantitative PCR and Western blotting were performed to analyze the expression of hypoxia-inducible factor 1α (HIF-1α),estrogen synthetase (CYP11A1,CYP17A1,and CYP19A1) genes and proteins.HIF-1α inhibitor LW6 was used to block HIF-1α synthesis,with cells divided into control group,FA group,and FA+LW6 group,after 12 h of in vitro culture，the expression of HIF-1α,CYP11A1,CYP17A1,and CYP19A1 genes and proteins in cumulus cells and estradiol concentration in supernatants of yaks were detected. 【Result】 Cytotoxicity assays showed that compared with 0 μmol/L FA group,5 μmol/L FA incubation for 12 h significantly increased cell viability and upregulated the expression of HIF-1α,CYP11A1,CYP17A1,and CYP19A1 genes and proteins in yak cumulus cells (P＜0.05).LW6 inhibition experiment revealed that compared with control group,FA treatment significantly increased the relative expression of HIF-1α,CYP11A1,CYP17A1,and CYP19A1 genes and proteins (except for CYP19A1) in yak cumulus cells (P＜0.05),the fluorescence intensity of HIF-1α was significantly enhanced,and the estradiol concentration was significantly increased (P＜0.05).FA+LW6 treatment significantly decreased the expression of all four genes and proteins in yak cumulus cells (P＜0.05),with significantly decreased estradiol concentration (P＜0.05). 【Conclusion】 FA could regulate the synthesis of estrogen synthase through the expression of HIF-1α gene,thereby promote estrogen secretion in yak cumulus cells.The results provided experimental evidence for FA as a natural reproductive regulator to improve yak breeding performance.

Key words: yak; cumuluscells; forsythiaside A (FA); hypoxia-induciblefactor-1α (HIF-1α); estrogen

CLC Number:

S814.1

YANG Juan, PAN Yangyang, WANG Meng, YANG Xiaoqing, LI Tianhao, MA Xin, ZHANG Xiaokun, CHANG Jianying, WANG Libin, FAN Jiangfeng. Effect of Forsythiaside A on Estrogen Synthase Secretion of Yak Cumulus Cells via HIF-1α[J]. China Animal Husbandry & Veterinary Medicine, 2025, 52(9): 4205-4215.

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Effect of Forsythiaside A on Estrogen Synthase Secretion of Yak Cumulus Cells via HIF-1α

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