贵州黑山羊AMH、CYP19A1基因多态性及其与产羔数的关联分析

doi:10.16431/j.cnki.1671-7236.2025.10.024

Abstract

Abstract: 【Objective】 The aim of this study was to analyze the polymorphism of anti-Müllerian hormone (AMH) and cytochrome P450,family 19,subfamily A,polypeptide 1 (CYP19A1) genes in Guizhou Black goats,and screen out the single nucleotide polymorphism (SNP) that had significant effects on the litter size of goats.【Method】 All the exons of AMH and CYP19A1 genes of Guizhou Black goats were screened by direct sequencing of PCR products,the secondary and tertiary structures of AMH and CYP19A1 proteins and signal peptides were predicted by online software,and the correlation between SNP and litter size was analyzed.【Result】 One SNP (g.2612 A＞G) was identified in the fifth exon of AMH gene in Guizhou Black goats.One SNP (g.37836 T＞C) was found in the fifth exon of CYP19A1 gene,and two SNPs (g.48986 C＞T and g.49046 A＞G) were discovered in the tenth exon of CYP19A1 gene.The secondary and tertiary structure prediction results revealed that the stability of the secondary structure of AMH and CYP19A1 proteins decreased after mutation,with the highest proportion of random coil,indicating higher complexity.Moreover,AMH and CYP19A1 proteins lacked signal peptides. χ² test results showed that all four SNPs deviated from the Hardy-Weinberg equilibrium (P＞0.05),and the polymorphism was moderate (0.25＜PIC＜0.5).The correlation analysis results showed that the litter size of GG genotype at g.2612 A＞G of AMH gene in Guizhou Black goats was extremely significantly or significantly higher than that of AG and AA genotypes (P＜0.01 or P＜0.05).The litter size of GG genotype at g.49046 A＞G of CYP19A1 gene in Guizhou Black goats was significantly higher than that of AA and AG genotypes (P＜0.05),while there was no significant difference in litter size among different genotypes of the other two SNPs (P＞0.05).【Conclusion】 g.2612 A＞G and g.49046 A＞G of AMH and CYP19A1 genes had significant association with the litter size of Guizhou Black goats,and could be used as molecular markers for the litter size of goats.The results provided a theoretical basis for the study of the multiple litter traits of Guizhou Black goats.

Key words: Guizhou Black goats; AMH gene; CYP19A1 gene; polymorphism; litter size

CLC Number:

S813.3

YANG Rong, ZHOU Di, WANG Zhenmei, TIAN Xingzhou, CHEN Xiang, LYU Yanli, WU Yu, AO Ye, GUO Zhengang, ZHAO Depeng, HUANG Qingyun, ZHANG Xing. Polymorphism of AMH and CYP19A1 Genes and Its Correlation Analysis with Litter Size in Guizhou Black Goats[J]. China Animal Husbandry & Veterinary Medicine, 2025, 52(10): 4785-4795.

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Polymorphism of AMH and CYP19A1 Genes and Its Correlation Analysis with Litter Size in Guizhou Black Goats

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