FABP3基因多态性及其对绵羊泌乳性状的影响

doi:10.16431/j.cnki.1671-7236.2025.11.023

Abstract

Abstract: 【Objective】 This study aimed to screen single nucleotide polymorphism (SNP) of fatty acid binding protein 3 (FABP3) gene,and analyze its association with lactation traits in sheep,providing fundamental data for the molecular genetic study of lactation traits. 【Method】 A total of 460 lactating sheep were selected,including 32 East Frisian sheep,56 Hu sheep,197 hybridization F1 of East Frisian×Hu sheep (F1),and 175 hybridization F2 of East Frisian×Hu sheep (F2).Blood samples were collected to extract DNA.After mixing the PCR amplification products in the pool,SNP of FABP3 gene were screened by Sanger sequencing and their genotypes were typed by penta-primer amplification refractory mutation system (PARMS) method.Finally,the effects of nucleotide sequence variation in FABP3 gene on lactation traits of sheep were analyzed. 【Result】 Two SNPs was found in introns 2 and 3 regions of FABP3 gene in sheep,which were c.246+20 G/A (SNP1) and c.348+12 C/T (SNP2),respectively.The genotyping results showed that SNP1 had three genotypes:AA,AG and GG,of which GG was the dominant genotype for F1 and F2 populations,while AG was the dominant genotype for Hu sheep,and G was the dominant allele for F1 and F2 populations.SNP2 had three genotypes:TT,TC and CC,of which TC was the dominant genotype for Hu sheep and F1 population,while TT was the dominant genotype for F2 population,and T was the dominant allele for F1 and F2 populations.Correlation analysis results indicated that the milk fat percentage of AG and GG genotypes of FABP3 gene in sheep was significantly higher than that of AA genotype (P＜0.05),and the allele G was significantly associated with increased milk fat percentage (P＜0.05).The milk fat percentages and average daily milk yields of TC and TT genotypes of FABP3 gene in sheep were significantly or extremely significantly higher than those of CC genotype (P＜0.05 or P＜0.01),and the allele T was significantly linked to increased milk fat content(P＜0.05).Furthermore,the average daily milk yield of CA haplotype of FABP3 gene in sheep was significantly higher than that of TG haplotype (P＜0.05). 【Conclusion】 In this study,the milk fat percentage of AG and GG genotypes at c.246+20 G/A of FABP3 gene in sheep was significantly higher than that of AA genotype,and the milk fat percentage and daily milk production of TC and TT genotypes at c.348+12 C/T in sheep were significantly higher than that of CC genotype.The results provided foundational data for molecular genetic research on lactation traits in sheep.

Key words: sheep; FABP 3 gene; nucleotide variation; lactation traits

CLC Number:

S813.3

GUO Shiwei, LIU Yuan, HAO Zhiyun, ZHANG Xiyun, CHE Longjie, LI Mingna, REN Chunyan, WANG Jiqing, WANG Zike. FABP3 Gene Polymorphism and Its Effect on Lactation Traits in Sheep[J]. China Animal Husbandry & Veterinary Medicine, 2025, 52(11): 5265-5275.

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FABP3 Gene Polymorphism and Its Effect on Lactation Traits in Sheep

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