基于转录组测序筛选浙籽鹅F2代繁殖性状相关候选基因

doi:10.16431/j.cnki.1671-7236.2025.11.022

Abstract

Abstract: 【Objective】 This study focused on the F2 generation female Zhezi geese derived from the crossbreeding of male Zhejiang White geese (♂) and female F1 generation Zhezi geese(♀),aiming to identify candidate genes related to reproductive traits through transcriptome analysis of blood samples collected during the laying and ceased periods,providing a reference for reproductive regulation in hybrid geese. 【Method】 Blood samples were collected from F2 generation Zhezi geese during the laying and ceased periods,and transcriptome sequencing (RNA-Seq) was performed using the Illumina NovaSeq 6000 sequencing platform.Differentially expressed genes (DEGs) were identified using DESeq,followed by GO function and KEGG pathway enrichment analysis to screen for genes associated with reproductive traits.Six differentially expressed genes were randomly selected for validation using Real-time quantitative PCR. 【Result】 Transcriptome sequencing results revealed a total of 4 576 differentially expressed genes between the laying and ceased periods,including 1 821 upregulated genes and 2 755 downregulated genes.GO function enrichment analysis identified 9 643 GO terms,primarily enriched in biological process such as positive regulation of calcium ion transmembrane transport,regulation of phagocytosis,and positive regulation of cation transmembrane transport,enriched in molecular functions including transferase activity,catalytic activity,purine nucleoside triphosphate binding,etc.,and enriched in cellular components mainly within intracellular.KEGG pathway enrichment analysis identified 157 pathways,mainly enriched in fructose and mannose metabolism,nicotinate and nicotinamide metabolism,and amino sugar and nucleotide sugar metabolism,with ADCY5,CD44,and CCND1 identified as candidate genes related to reproductive traits.Real-time quantitative PCR results were consistent with the transcriptome sequencing trends,confirming the reliability of the sequencing data. 【Conclusion】 This study identified three candidate genes (ADCY5,CD44,and CCND1) associated with reproductive traits through transcriptome sequencing,providing a foundation for functional research and subsequent molecular regulatory mechanisms in F2 generation Zhezi geese.

Key words: F2 generation Zhezi geese; transcriptome; signal pathway; candidate gene

CLC Number:

S835.3

YUE Shan, ZHAO Xiuhua, SUN Jinyan, PENG Fugang, ZHANG Yuanliang, LI Manyu, LIU Guojun. Screening of Candidate Genes Related to Reproductive Traits in F2 Generation Zhezi Geese Based on Transcriptome Sequence[J]. China Animal Husbandry & Veterinary Medicine, 2025, 52(11): 5254-5264.

References

[1] SONG Y P,MA J Y,LIU Q Y,et al.Protein profile analysis of Jilin White goose testicles at different stages of the laying cycle by DIA strategy[J].BMC Genomics,2024,25(1)：326.
[2] 万伟粲，张旭，黄璇，等.基于全基因组重测序分析酃县白鹅保种效果[J].中国畜牧兽医，2024，51(11)：4890-4898.WAN W C，ZHANG X，HUANG X，et al.Assessment of conservation effects of Lingxian White goose based on whole genome resequencing[J].China Animal Husbandry ＆ Veterinary Medicine，2024，51(11)：4890-4898.(in Chinese)
[3] NZABANITA D,SHEN H,GRIST S,et al.Heavy metal concentrations in feathers and metabolomic profiles in Pacific Black ducks (Anas superciliosa) from Southeastern Australia[J].Environmental Toxicology and Chemistry,2025，44(1)：92-102.
[4] YANG L，JIA C Z，LI Y Z，et al.The hypothalamic transcriptome reveals the importance of visual perception on the egg production of Wanxi White geese[J].Frontiers in Veterinary Science，2024，11：1449032.
[5] 孙燕勇，马凤英，郭丽丽，等.动物血液转录组测序的应用研究进展[J].中国畜牧兽医，2023，50(2)：460-468.SUN Y Y，MA F Y，GUO L L，et al.Advances on the application of animal blood transcriptome[J].China Animal Husbandry ＆ Veterinary Medicine，2023，50(2)：460-468.(in Chinese)
[6] 康彦东，郭宪.血液转录组和代谢组在家畜繁殖中的研究进展[J].中国草食动物科学，2024，44(1)：29-33.KANG Y D，GUO X.Research progress of blood transcriptome and metabolome in livestock breeding[J].China Herbivore Science，2024，44(1)：29-33.(in Chinese)
[7] 孙慧敏，范冰峰，张旭林，等.梅花鹿早期妊娠不同时期血液转录组分析[J].中国畜牧兽医，2024，51(9)：3892-3908.SUN H M，FAN B F，ZHANG X L，et al.Transcriptomic analysis of blood in early pregnancy at different time points in sika deer[J]. China Animal Husbandry ＆ Veterinary Medicine，2024，51(9)：3892-3908.(in Chinese)
[8] DICKINSON S E,GRIFFIN B A,ELMORE M F,et al.Transcriptome profiles in peripheral white blood cells at the time of artificial insemination discriminate beef heifers with different fertility potential[J].BMC Genomics,2018,19(1)：129.
[9] 李婉卿，曾亚琦，姚新奎，等.肉用型伊犁马的血液转录组比较分析[J].畜牧兽医学报，2024，55(7)：2951-2962.LI W Q，ZENG Y Q，YAO X K，et al.Comparative analysis of blood transcriptome in Yili horses bred for meat performance[J].Acta Veterinaria et Zootechnica Sinica，2024，55(7)：2951-2962.(in Chinese)
[10] 陈雪艳，木合塔帕·买买提江，李政，等.基于血液转录组探究皮山红羊繁殖性状候选基因的差异表达[J].中国畜牧杂志，2024，60(11)：185-193.CHEN X Y，MAIMAITIJIANG M，LI Z，et al.Differential expression of reproductive trait candidate genes in Pishan Red sheep based on blood transcriptome[J].Chinese Journal of Animal Science，2024，60(11)：185-193.(in Chinese)
[11] 任倩俐，苏凯，姚丽，等.褐马鸡繁殖期和非繁殖期血液转录组的比较分析[J].特种经济动植物，2023，26(2)：1-15.REN Q L，SU K，YAO L，et al.Comparative analysis of blood transcriptome of Brown eared pheasant in reproductive period and non-reproductive period[J].Special Economic Animals and Plants，2023，26(2)：1-15.(in Chinese)
[12] GUMUłKA M,AVITAL-COHEN N,ROZENBOIM I.Determination of annual plasma hormone levels associated with reproduction in long-day breeding domestic geese[J].Animals，2021，11(8)：2363.
[13] LIU G J,GUO Z H,ZHAO X H,et al.Whole genome resequencing identifies single-nucleotide polymorphism markers of growth and reproduction traits in Zhedong and Zi crossbred geese[J].Genes,2023,14(2)：487.
[14] 耿亚非.骨母细胞瘤临床病理分析以及FOS基因融合在肿瘤中的作用机制研究[D].上海：海军军医大学，2023.GENG Y F.Clinicopathological analysis of osteoblastoma and the mechanism of FOS gene fusion in tumorigenesis[D].Shanghai：Naval Medical University，2023.(in Chinese)
[15] KIM D，LANGMEAD B，SALZBERG S L.HISAT:A fast spliced aligner with low memory requirements[J].Nature Methods，2015，12(4)：357-360.
[16] LIU H，ZHU C，SONG W，et al.Genomic characteristics of four different geese populations in China[J].Animal Genetics，2021，52(2)：228-231.
[17] CHEN Z，WEN D，ZHANG Y，et al.Pituitary transcriptome profile from laying period to incubation period of Changshun Green-shell laying hens[J]. BMC Genomics,2024,25(1)：309.
[18] 高志浩.籽鹅繁殖轴开产相关基因筛选和功能分析[D].大庆：黑龙江八一农垦大学,2021.GAO Z H.Screening and functional analysis of genes related to HPG axis of Zi geese[D].Daqing：Heilongjiang Bayi Agriculutual University,2021.(in Chinese)
[19] LIU Y，WANG X Z，LI G Q，et al.Investigating the impact of fasting and refeeding on blood biochemical indicators and transcriptional profiles in the hypothalamus and subcutaneous adipose tissue in geese[J].Animals，2024，14(18)：2746.
[20] FRESARD L，SMAIL C，FERRARO N M，et al.Identification of rare-disease genes using blood transcriptome sequencing and large control cohorts[J]. Nature Medicine，2019，25(6)：911-919.
[21] DEFER N，BEST-BELPOMME M，HANOUNE J.Tissue specificity and physiological relevance of various isoforms of adenylyl cyclase[J].American Journal of Physiology-Renal Physiology，2000，279：400-416.
[22] BELLO S F,XU H P，GUO L J，et al.Hypothalamic and ovarian transcriptome profiling reveals potential candidate genes in low and high egg production of White Muscovy ducks (Cairina moschata)[J].Poultry Science，2021，100(9)：101310.
[23] LI J，SHEN C，ZHANG K，et al.Polymorphic variants of bovine ADCY5 gene identified in GWAS analysis were significantly associated with ovarian morphological related traits[J].Gene，2021，766：145158.
[24] ZHANG G，FEENSTRA B，BACELIS J，et al.Genetic associations with gestational duration and spontaneous preterm birth[J].New England Journal of Medicine，2017，377(12)：1156-1167.
[25] ZHOU G，HOLZMAN C，HENG Y J J，et al.EBF1 gene mRNA levels in maternal blood and spontaneous preterm birth[J].Reproductive Science，2020，27：316-324.
[26] YAGHOBI Z，MOVASSAGHPOUR A，TALEBI M，et al.The role of CD44 in cancer chemoresistance:A concise review[J].European Journal of Pharmacology，2021，903：174147.
[27] XIANG X，HUANG X，WANG J，et al.Transcriptome analysis of the ovaries of Taihe Black-bone silky fowls at different egg-laying stages[J].Genes，2022，13(11)：2066.
[28] CHAEN T，KONNO T，EGASHIRA M，et al.Estrogen-dependent uterine secretion of osteopont in activates blastocyst adhesion competence[J].PLoS One，2012，7(11)：e48933.
[29] RAHEEM K A，MAREI W F，MIFSUD K，et al.Regulation of the hyaluronan system in ovine endometrium by ovarian steroids[J].Reproduction，2013，145：491-504.
[30] GUPTA S，PANDEY S，PARMAR M S，et al.Impact of oocyte-secreted factors on its developmental competence in buffalo[J].Zygote，2017，25(3)：313-320.
[31] JIANG P，FANG X，XIA L，et al.The effect of CD44 gene on triglyceride synthesis of bovine mammary epithelial cells[J].Journal of Animal Science，2018，96：136-137.
[32] SUN Y，WU X，MA Y，et al.Molecular marker-assisted selection of ABCG2, CD44,SPP1 genes contribute to milk production traits of chinese holstein[J].Animals，2022，13(1)：89.
[33] MJELLE R，HEGRE S A，AAS P A，et al.Cell cycle regulation of human DNA repair and chromatin remodeling genes[J]. DNA Repair，2015，30(9)：53-67.
[34] SUN X，LISWANISO S，SHAN X S，et al.The opposite effects of VGLL1 and VGLL4 genes on granulosa cell proliferation and apoptosis of hen ovarian prehierarchical follicles[J].Theriogenology，2022，181：95-104.
[35] MIZUSHIMA S，SASANAMI T，ONO T，et al.Cyclin D1 gene expression is essential for cell cycle progression from the maternal-to-zygotic transition during blastoderm development in Japanese quail[J].Developmental Biology，2021，476：249-258.
[36] 石仙.基于单细胞测序的卵丘颗粒细胞中CCND1和CCND2对牦牛卵母细胞发育影响的研究[D].成都：西南民族大学，2017.SHI X.Effects of CCND1 and CCND2 in cumulus-granulosa cell on the development of yak oocytes by single-cell sequencing analysis[D].Chengdu：Southwest Minzu University，2017.(in Chinese)
[37] YANG Q，LU X，LI G，et al.Genetic analysis of egg production traits in Luhua chickens:Insights from a multi-trait animal model and a genome-wide association study[J].Genes，2024，15：796.

Screening of Candidate Genes Related to Reproductive Traits in F2 Generation Zhezi Geese Based on Transcriptome Sequence

PDF (PC)

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 15

Recommended Articles

Metrics

Comments

[1]	ZHAO Runze, MIN Zhaoling, NIU Naiqi, ZONG Wencheng, YAN Yibo, ZHANG Longchao. Identification of Major Genes Affecting the Number of Thoracic Vertebrae in Beijing Black Pigs Based on the Joint Analysis of GWAS and Embryonic Gene Expression Differences in Divergently Selected Lines [J]. China Animal Husbandry & Veterinary Medicine, 2025, 52(9): 4182-4194.
[2]	LEI Xingfen, LI Shun, HUANG Yunfei, LI Yajuan, SUN Qinqin, FU Qiang. Research Progress on the Mechanism of Autophagy in Bacterial Infection [J]. China Animal Husbandry & Veterinary Medicine, 2025, 52(9): 4505-4514.
[3]	TIAN Jindong, TA La, LI Weiwei, XU Huijuan, WU Jianghong, WUDUBALA, HU Sile, CAO Wenya, SONG Hongli, FU Shaoyin, LI Chun. Screening of Candidate Genes Related to Cashmere Shedding in Cashmere Goats Based on Transcriptome and Proteome Analysis [J]. China Animal Husbandry & Veterinary Medicine, 2025, 52(8): 3497-3514.
[4]	DENG Yunqi, SHI Bingang, ZHAO Zhidong, HU Jiang, ZHANG Xiaolan. Transcriptome Analysis of Tianzhu White Yak Skin at Different Age Stages and Identification of Immune-regulating Genes [J]. China Animal Husbandry & Veterinary Medicine, 2025, 52(8): 3515-3526.
[5]	MIAO Na, QIAO Jiakun, YANG Hui, HAN Pingping, XU Fangjun, CHE Zhaoxuan, DAI Xiangyu, XU Minghang, LONG Zhiwei, ZHU Mengjin. Genome Wide Association Study of Immune Traits in Duroc×Erhualian F2 Generation Pigs [J]. China Animal Husbandry & Veterinary Medicine, 2025, 52(4): 1455-1467.
[6]	LIU Sirui, LIU Hongfei, LIU Dapeng, MU Qiming, TANG Hehe, ZHANG He, ZHANG Yongfu, HOU Shuisheng, ZHOU Zhengkui. Screening of Candidate Genes Regulating Fat Deposition in Pekin Ducks by Integrating Genome and Transcriptome [J]. China Animal Husbandry & Veterinary Medicine, 2025, 52(4): 1468-1477.
[7]	WU Min, XU Junjie, LI Xinxin, CHEN Yifan, WANG Dehe, HAO Erying, CHEN Hui, SHI Lei. Screening of Key Genes Regulating Muscle Development in Yellow-feathered Chickens Based on RNA-Seq [J]. China Animal Husbandry & Veterinary Medicine, 2025, 52(3): 990-1000.
[8]	DU Jiawen, REN Wenyi, XU Xiaofeng, ZHANG Lili. Comparison of the Transcriptome of Longissimus Dorsi Muscle of Tan Sheep at Different Growth Stages [J]. China Animal Husbandry & Veterinary Medicine, 2025, 52(3): 1011-1021.
[9]	CHEN Xinyou, WU Pingxian, WU Xiaoqian, ZHANG Tinghuan, A Chao, WU Xueshan, SHI Rui, LIU Lan, CHEN Li, GUO Zongyi, WANG Jinyong, DIAO Shuqi. Analysis of Selection Signatures for Rongchang Pigs Across the Whole Genome Based on SNP Chip [J]. China Animal Husbandry & Veterinary Medicine, 2025, 52(11): 5021-5029.
[10]	KANG Zhen, MENG Chu, YU Yongxin, LIU Qi, TU Feng, ZHAO Ruqian, JIA Yimin. Transcriptome Analysis of the Effects of Dietary Curcumin and Tetrahydrocurcumin on Backfat Thickness of Fattening Pigs [J]. China Animal Husbandry & Veterinary Medicine, 2025, 52(10): 4677-4687.
[11]	HU Ziping, SU Yanfang, ZONG Wencheng, NIU Naiqi, ZHNAG Longchao, WANG Yuan. Screening of Candidate Genes for Two-end Black Coat Color in Jianhe White Xiang Pigs Based on Transcriptome and Genome Data [J]. China Animal Husbandry & Veterinary Medicine, 2025, 52(1): 13-24.
[12]	SUN Huimin, FAN Bingfeng, ZHANG Xulin, SHAO Jing, LIU Lixiang, XU Baozeng. Transcriptomic Analysis of Blood in Early Pregnancy at Different Time Points in Sika Deer [J]. China Animal Husbandry & Veterinary Medicine, 2024, 51(9): 3892-3908.
[13]	WANG Ying, CHEN Jing, MIAO Dongzhi, ZHANG Chi, CHEN Junhong, YANG Haiming, WANG Zhiyue. Transcriptome Construction of F3 Follicle and Analysis of Genes Related to Follicle Atresia in Pigeons [J]. China Animal Husbandry & Veterinary Medicine, 2024, 51(9): 3921-3929.
[14]	ZHAO Yutian, CHEN Fuju, YI Pingchang, MA Min, WANG Shouning, QUAN Qishiliu. Exploring Candidate Genes Related to Kidney-adjacent Lipid Deposition in Yak Calves Based on Transcriptome Sequencing [J]. China Animal Husbandry & Veterinary Medicine, 2024, 51(7): 2727-2738.
[15]	WANG Shunbo, WANG Hao, LIANG Yunyi, WANG Wenjing, LI Zicong, XU Zheng. Effects of Feeding Risperidone During Late Pregnancy on Mammary Gland Development of Maternal Mice and Growth of Offspring Mice [J]. China Animal Husbandry & Veterinary Medicine, 2024, 51(6): 2489-2500.