杜洛克猪平均日增重的全基因组关联分析

doi:10.16431/j.cnki.1671-7236.2025.10.027

Abstract

Abstract: 【Objective】 Candidate genes associated with average daily gain (ADG) in Duroc pigs were identified using a genome-wide association study (GWAS),and colocalization analysis was performed by integrating gene expression regulatory data to gain deeper insights into the genetic regulatory mechanisms underlying ADG.【Method】 A GWAS was conducted based on ADG phenotypic records from 1 346 Duroc pigs and genotypic data generated using the Zhongxin No.1 50K SNP chip to identify quantitative trait locus (QTL) and associated candidate genes for ADG.GO and KEGG enrichment analyses were employed to explore the biological functions of the candidate genes,and colocalization analysis was performed by integrating expression quantitative trait locus (eQTL) data from 34 tissues provided by the pig genotype-tissue expression (PigGTEx) project,to identify potential causal genes and functionally relevant tissues contributing to the genetic regulation of ADG.【Result】 A total of 1 530 significant single nucleotide polymorphisms (SNPs) (P＜1×10^-5) associated with ADG were identified through GWAS and were mapped to 50 QTL, distributed across chromosomes 1,3-8,10-13,and 15-18.The QTL regions were annotated through the Ensembl database,resulting in the identification of 513 candidate genes.GO and KEGG enrichment analyses revealed that ALPK2,TULP3,and TMOD4 genes were significantly enriched in pathways related to limb development and muscle cell development.Colocalization analysis further revealed that three potential causal variants influence ADG by regulating the expression of MVP,PRR14,and DDX11 genes in different tissues.【Conclusion】 In this study,key QTL regions and potential causal genes associated with ADG in Duroc pigs were identified through the integration of GWAS and colocalization analysis.These findings provided important reference information for the genetic improvement of ADG in Duroc pigs.

Key words: Duroc pigs; genome-wide association studies; average daily gain; colocalization

CLC Number:

S813.3

ZHANG Changliang, XU Zhiting, GUO Kaixuan, SU Chao, NING Wenzhe, SI Jinglei, ZHOU Jun, GAO Yahui, ZHANG Zhe. Genome-wide Association Study of Average Daily Gain in Duroc Pigs[J]. China Animal Husbandry & Veterinary Medicine, 2025, 52(10): 4822-4829.

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Genome-wide Association Study of Average Daily Gain in Duroc Pigs

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