中国畜牧兽医 ›› 2026, Vol. 53 ›› Issue (2): 723-734.doi: 10.16431/j.cnki.1671-7236.2026.02.020

• 营养与饲料 • 上一篇    下一篇

饲粮中添加石榴皮粉对肉鸡生长性能、抗氧化能力及肠道微生物的影响

易林1,2(), 周长明2(), 亢守亭2, 赵付伟2, 赵宪林2, 万双秀2, 姜莉莉2, 王世宇2, 樊兆斌2(), 张建斌1()   

  1. 1.天津农学院动物科学与动物医学学院,天津 300000
    2.菏泽学院,菏泽 274015
  • 收稿日期:2025-07-15 出版日期:2026-02-20 发布日期:2026-01-27
  • 通讯作者: 樊兆斌,张建斌 E-mail:yilinhn2023@163.com;306716753@qq.com;fanzhaobin79@163.com;zjbzwz@126.com
  • 作者简介:易林,E-mail:yilinhn2023@163.com
    周长明,E-mail:306716753@qq.com
    第一联系人:(易 林、周长明并列第一作者)
  • 基金资助:
    中央引导地方科技发展资金“现代生猪重要疫病净化与高效健康养殖关键技术示范”(YDZX2023069);2022年山东本科教学改革重点项目“睿鹰生物医药产业学院‘四四三三’建设模式创新研究与实践 ”(Z202213)

Effects of Pomegranate Peel Powder on Growth Performance, Antioxidant Capacity and Intestinal Microorganisms of Broilers

YI Lin1,2(), ZHOU Changming2(), KANG Shouting2, ZHAO Fuwei2, ZHAO Xianlin2, WAN Shuangxiu2, JIANG Lili2, WANG Shiyu2, FAN Zhaobin2(), ZHANG Jianbin1()   

  1. 1.College of Animal Science and Veterinary Medicine,Tianjin Agricultural University,Tianjin 300000,China
    2.Heze University,Heze 274015,China
  • Received:2025-07-15 Online:2026-02-20 Published:2026-01-27
  • Contact: FAN Zhaobin, ZHANG Jianbin E-mail:yilinhn2023@163.com;306716753@qq.com;fanzhaobin79@163.com;zjbzwz@126.com

摘要:

目的 探究饲粮中添加石榴皮粉(PPP)对白羽肉鸡生长性能、肠道形态、血清生化与抗氧化指标及盲肠菌群结构的影响。 方法 选取288只1日龄爱拔益加(AA)肉鸡,随机分为4组,每组3个重复,每重复24只鸡。对照组(CON)饲喂基础饲粮,1、2和3组分别在基础饲粮中添加0.75%、1.5%和3.0%的PPP,试验期42 d。于试验开始和结束时称重,记录采食量,计算平均日增重(ADG)、平均日采食量(ADFI)和料重比(F/G)。试验结束后,每组随机选取12只鸡,采集血液并分离血清、收集空肠和回肠组织及盲肠内容物,测定血清生化、抗氧化指标,观察肠道形态结构,并利用16S rDNA测序分析盲肠微生物组成。 结果 与对照组相比,1组肉鸡生长性能有改善趋势,但差异不显著(P>0.05)。与对照组相比,1组肉鸡血清天冬氨酸氨基转移酶(AST)活性显著降低(P<0.05),超氧化物歧化酶(SOD)活性和总抗氧化能力(T-AOC)显著提高(P<0.05)。与对照组相比,1组肉鸡空肠绒毛高度(VH)及绒隐比(V/C)显著上升(P<0.05)。盲肠微生物测序结果显示,0.75% PPP可显著提升菌群丰富度与多样性,促进拟杆菌属(Bacteroides)、双歧杆菌属(Bifidobacterium)和克里斯滕森氏菌属(Christensenella)等有益菌的增殖(P<0.05),显著降低潜在致病菌另枝菌属(Alistipes)丰度(P<0.05)。相关性分析表明,拟杆菌属和双歧杆菌属与血清抗氧化指标和肠道形态参数均呈正相关。 结论 本试验条件下,饲粮中添加0.75% PPP能够显著增强肉鸡机体的抗氧化能力、改善肠道黏膜形态结构,并提高盲肠有益菌群的物种丰富度。

关键词: 石榴皮; 肠道形态; 血清生化; 抗氧化能力; 肠道菌群

Abstract:

Objective This study aimed to investigate the effects of dietary supplementation with pomegranate peel powder (PPP) on the growth performance, intestinal morphology, serum biochemical and antioxidant indices, and cecal microbiota structure of White-feathered broilers. Method A total of 288 one-day-old Arbor Acres (AA) broilers were randomly divided into 4 groups with 3 replicates per group and 24 broilers per replicate. The control group (CON) was fed a basal diet, while groups 1, 2 and 3 received the basal diet supplemented with 0.75%, 1.5% and 3.0% PPP, respectively. The trial lasted for 42 days. Body weight and feed intake were recorded at the beginning and end of the trial to calculate average daily gain (ADG), average daily feed intake (ADFI), and feed-to-gain ratio (F/G). At the end of the experiment, 12 chickens were randomly selected. Blood was collected and serum was sparated. Collect jejunal and ileal tissues as well as cecal contents and determine serum biochemical and antioxidant indices, intestinal morphology, and cecal microbial composition (analyzed via 16S rDNA sequencing) were determined. Result Compared with CON group, the growth performance of broilers in group 1 showed an improving trend, but the difference was not significant (P>0.05). Compared with CON group, the activity of aspartate aminotransferase (AST) in the serum of broilers in group 1 was significantly decreased (P<0.05), while the activity of superoxide dismutase (SOD) and total antioxidant capacity (T-AOC) were significantly increased (P<0.05). Compared with CON group, the villus height (VH) and villus/crypt ratio (V/C) of the jejunum in group 1 broilers were significantly increased (P<0.05). The results of cecal microbiota sequencing showed that 0.75% PPP could significantly increase the richness and diversity of the microbiota, promote the proliferation of beneficial bacteria such as BacteroidesBifidobacterium and ChristensenellaP<0.05), and significantly reduce the abundance of the potential pathogen AlistipesP<0.05). Correlation analysis indicated that Bacteroides and Bifidobacterium were positively correlated with serum antioxidant indicators and intestinal morphology parameters. Conclusion Under the present experimental conditions, dietary supplementation with 0.75% PPP could significantly enhance antioxidant capacity, improve intestinal mucosal morphology, and increase the richness of beneficial cecal microbiota in broilers.

Key words: pomegranate peel; intestinal morphology; serum biochemistry; antioxidant capacity; intestinal flora

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