武夷黑猪HSPA5基因克隆、生物信息学分析及组织表达研究

doi:10.16431/j.cnki.1671-7236.2025.07.022

Abstract

Abstract: 【Objective】 The heat shock protein family A member 5 (HSPA5) of Wuyi Black pig was analyzed through bioinformatics predictions to provide insights on its biological functions. 【Method】 The coding sequence of HSPA5 gene was amplified and sequenced,similarity analysis was performed afterward,then a phylogenetic tree was constructed.The physicochemical properties,structural features,and functional roles of the HSPA5 protein were predicted using bioinformatics software,and its expression in different tissues of Wuyi Black pig were finally measured. 【Result】 The coding sequence of HSPA5 gene in Wuyi Black pig was 1 965 bp,encoding 654 amino acids.The results of similarity and phylogenetic tree analyses showed that the amino acid sequence of HSPA5 in Wuyi Black pig had the highest similarity (100%) with the transcript variant 2 of HSPA5 in Duroc pig and that in mice.It had the closest genetic relationship with Mus musculus and the most distant genetic relationship with Larimichthys crocea.The HSPA5 protein was predicted as an acidic hydrophilic protein containing a signal peptide sequence,with the highest concentration observed in the cytoplasm.It was mainly composed of alpha helix conformation with conserved motifs within two overlapping functional domains.It contained 56 phosphorylation sites,76 O-glycosylation sites and 24 chemical interaction sites,as well as 26 antigenic determinants,11 T-cell epitopes,and 13 B-cell epitopes.It was closely related to interactions with heat shock protein member 90 (HSP90) and activating transcription factor 6 (ATF6).The Real-time quantitative PCR results showed that the expression of HSPA5 gene in liver and uterus of Wuyi Black pig was significantly higher than that in other tissues (P＜0.05). 【Conclusion】 The HSPA5 gene of Wuyi Black pig had been successfully cloned,and had the closest genetic relationship with that of Mus musculus.It was predominantly localized in the cytoplasm,featuring phosphorylation and O-glycosylation modification sites,it also interacted with proteins related to the unfolded protein response pathway.It had the highest expression in liver of Wuyi Black pig.These characteristics could provide valuable references for further studies on the biological functions of HSPA5 protein.

Key words: Wuyi Black pig; HSPA5 gene; bioinformatics; function; tissue expression

CLC Number:

S828.8⁺9

LI Zhiquan, FU Sijing, XU Shuping, GAO Mengruo, YANG Taotao, ZHANG Zhibang, LI Kai, LI Pengcheng. Cloning, Bioinformatics Analysis and Tissue Expression of HSPA5 Gene in Wuyi Black Pig[J]. China Animal Husbandry & Veterinary Medicine, 2025, 52(7): 3214-3224.

References

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Cloning, Bioinformatics Analysis and Tissue Expression of HSPA5 Gene in Wuyi Black Pig

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