China Animal Husbandry & Veterinary Medicine ›› 2025, Vol. 52 ›› Issue (5): 2056-2065.doi: 10.16431/j.cnki.1671-7236.2025.05.011

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Effects of Chestnut Bur Aqueous Extracts on Growth Performance,Organ Coefficient,Antioxidant Function and Tissue Structure in Mice

ZHAO Na1, WANG Lizhong2, ZHU Lianying1, CAI Yumei2, LI Shuang3, LI Chenghui1   

  1. 1. Faculty of Life Science, Tangshan Normal University, Tangshan 063000, China;
    2. Ocean College of Tangshan Normal University, Tangshan 063000, China;
    3. School of Public Health, North China University of Science and Technology, Tangshan 063210, China
  • Received:2024-10-21 Online:2025-05-05 Published:2025-04-27

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Abstract: 【Objective】 This experiment was designed to investigate the effects of chestnut bur aqueous extracts (CBE) on growth performance,organ coefficient,antioxidant function and tissue structure in mice,and provide reference for the development and application of CBE in animal production.【Method】 Forty 4-week-old SPF Kunming mice were randomly divided into 5 groups of 8 mice each.Mice in control group were given normal drinking water,and mice in experimental groups were supplemented with 0.05%,0.10%,0.25% and 0.50% CBE in drinking water,respectively.The test was maintained for 28 days.After the experiment,the average daily gain (ADG),average daily feed intake (ADFI),feed-to-gain ratio (F/G) and average daily water intake (ADWI) in each group were calculated.Blood was collected by picking eyeball,and mice were sacrificed by cervical dislocation method.Heart,spleen,thymus,kidney and liver were isolated,and organ coefficients were calculated.Tissue structure of liver and spleen was observed by HE staining,and antioxidant indicators in serum and liver were detected.【Result】 Compared with control group,①CBE had no significant effects on final body weight,ADG,ADFI,F/G and ADWI in mice (P＞0.05).②CBE had no significant effects on the indices of heart,spleen,thymus and kidney (P＞0.05).③There were no significant pathological changes of tissue structures in liver and spleen of mice in 0.05% and 0.10% CBE groups.Inflammatory cell infiltration and slight widening of hepatic sinusoids were observed in liver of 0.25% and 0.50% CBE groups.The spleen tissue structure was relatively disordered,and the white pulp area in 0.25% and 0.50% CBE groups was significantly atrophied.④The activity of glutathione peroxidase (GSH-Px) in serum of mice in 0.10%,0.25% and 0.50% CBE groups was significantly increased (P＜0.05).The activity of total superoxide dismutase (T-SOD) in serum of mice in 0.05% and 0.10% CBE groups was significantly increased (P＜0.05).The content of malondialdehyde (MDA) in serum of mice in 0.10% and 0.25% CBE groups was significantly decreased (P＜0.05),and the activity of catalase (CAT) was significantly increased (P＜0.05).The total antioxidant capacity (T-AOC) had no significant difference in serum of mice in experimental groups (P＞0.05).⑤The GSH-Px activity in liver of mice in 0.10% CBE group was significantly increased (P＜0.05),and the T-SOD activity in 0.05%,0.10% and 0.25% CBE groups was significantly increased (P＜0.05).There were no significant change of MDA content in liver of mice in experimental groups (P＞0.05).The activity of CAT and T-AOC in liver of mice in 0.10% CBE group was significantly increased (P＜0.05).【Conclusion】 Under the condition of this experiment,0.10% CBE addition to drinking water could enhance the antioxidant capacity of mice without affecting their growth performance and organ coefficients,and there were no visible damage of the tissue structure of liver and spleen.

Key words: chestnut bur aqueous extract; mice; growth performance; antioxidant function; tissue structure

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