China Animal Husbandry & Veterinary Medicine ›› 2025, Vol. 52 ›› Issue (7): 3004-3015.doi: 10.16431/j.cnki.1671-7236.2025.07.004

• Biotechnology • Previous Articles     Next Articles

Cloning, Bioinformatics and Tissue Expression Analysis of ARHGDIB Genes in Ducks

ZHENG Hao1, LIU Mengyun1,2, WANG Mingyu1, DONG Xia1, SHI Liangyu1, YU Bo1, YANG Yu2, ZHOU Ao1, CHEN Xing2   

  1. 1. Laboratory of Genetic Breeding Reproduction and Precision Livestock Farming, School of Animal Science and Nutritional Engineering, Wuhan Polytechnic University, Wuhan 430023, China;
    2. Institute of Animal Husbandry and Veterinary, Wuhan Academy of Agricultural Sciences, Wuhan 430208, China
  • Received:2024-09-20 Online:2025-07-05 Published:2025-07-01

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Abstract: 【Objective】 This study aimed to investigate the functional role of the duck Rho GDP dissociation inhibitor β (ARHGDIB) gene and further elucidate the molecular mechanisms underlying subcutaneous fat deposition in ducks. 【Method】 The ARHGDIB gene was cloned from the liver tissue cDNA of 28-day-old Wuqin-10 meat ducks,and was verified to be correct by agarose gel electrophoresis and sequencing.The obtained sequences were compared and bioinformatics analyzed using online software.The relative expression of ARHGDIB gene in thymus,lung,spleen,heart,duodenum,liver,kidney,abdominal fat,pectoralis muscles,leg muscle,and subcutaneous fat of ducks was detected by Real-time quantitative PCR. 【Result】 The full length of ARHGDIB gene in ducks was 1 409 bp,with a predicted coding region of 693 bp spanning 7 exons,encoding 230 amino acids.Leucine was the most abundant amino acid (10% of the total composition).Nucleotide sequence similarity of the ARHGDIB gene in ducks with that of Anser cygnoides,Bos taurus,Canis lupus familiaris,Capra hircus,Danio rerio,Equus caballus,Felis catust,Gallus gallus,Homo sapiens,Macaca fascicularis,Mus musculus,Ovis aries,Pan troglodytes,Papio anubis,Sus scrofa,Columba livia were 82.56%,50.53%,58.75%,54.08%,24.94%,56.30%,35.31%,81.79%,54.10%,54.90%,49.44%,47.25%,56.22%,55.99%,55.17%,and 82.64%,respectively.The phylogenetic tree analysis showed that ducks were most closely related to Anser cygnoides,followed by Columba livia.The ARHGDIB protein in ducks was a stable hydrophilic protein lacking transmembrane domains.It contained 24 phosphorylation sites and 2 N-glycosylation sites.Subcellular localization prediction suggested that the protein was predominantly localized to the mitochondria.The secondary structure of the ARHGDIB protein in ducks comprises α-helices,extended chain,β-turns,and random coils,with respective proportions of 25.22%,23.91%,4.35% and 46.52%.The predicted tertiary structure aligned with the secondary structure.Real-time quantitative PCR results demonstrated that the ARHGDIB gene was ubiquitously expressed across all examined tissues of ducks,with the highest expression observed in the thymus,which was significantly higher than in other tissues (P＜0.05).Moderately high expression was detected in the spleen,heart,and lung,while subcutaneous fat showed the lowest expression,significantly lower than all other tissues (P＜0.05). 【Conclusion】 The coding region of ARHGDIB gene in ducks was 693 bp long and encodes 230 amino acids.ARHGDIB protein in ducks was hydrophilic and predominantly localized to mitochondria.The ARHGDIB gene was expressed in various tissues of ducks,with the highest levels in thymus and the lowest level in subcutaneous fat.These findings provided a theoretical foundation for further investigation of ARHGDIB gene function in ducks,and establish a basis for elucidating its regulatory role in lipid metabolism and supporting targeted duck breeding programs.

Key words: ducks; ARHGDIB gene; cloning; bioinformatics analysis; tissue expression

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