细粒棘球绦虫MAPⅡ生物信息学分析及其在虫体不同阶段表达差异研究

doi:10.16431/j.cnki.1671-7236.2025.07.029

Abstract

Abstract: 【Objective】 The purpose of this experiment was to study the molecular characteristics,reactogenicity and expression of methionine aminopeptidase Ⅱ (MAP Ⅱ) in different stages of Echinococcus granulosus development,and provide a vaccine candidate antigen with protective effect on the definitive host. 【Method】 Bioinformatics analysis of MAP Ⅱ protein was carried out,including phylogenetic tree construction,physical and chemical properties analysis,and prediction of signal peptide,transmembrane region,antigen epitopes,phosphorylation and glycosylation sites,and secondary and tertiary structures.The prokaryotic system was used to express MAP Ⅱ protein and the antigenicity of the recombinant protein was verified.At the same time,the transcript levels of different developmental stages of Echinococcus granulosus were analyzed. 【Result】 The MAP Ⅱ protein of Echinococcus granulosus was distantly related to that of the definitive host dog and the intermediate host sheep.MAPⅡ was composed of 453 amino acids with a molecular weight of 51 023.75 u.The instability coefficient was 36.6,indicating Map Ⅱ was a stable protein.The average hydrophobicity index was －0.609 and the hydrophilicity was good.There was no signal peptide and transmembrane region in MAP Ⅱ protein.Map Ⅱ protein had a good B cell linear epitope,with a total of 38 phosphorylation sites and 1 N-glycosylation site.The secondary structure of MAPⅡ protein was dominated by alpha helix and random coil,accounting for 33.55% and 51.88%,respectively.The tertiary structure prediction showed that it was a monomeric protein,and the GMQE value was 0.85.The MAP Ⅱ recombinant protein expressed by prokaryotic system could bind to the positive serum of dogs infected with Echinococcus granulosus,and had good antigenicity.MAP Ⅱ protein was expressed in both the protoscoleces stage and the adult stage,and the expression in the adult stage was significantly higher than that in the protoscoleces stage (P＜0.05). 【Conclusion 】 Map Ⅱ protein had stable structure and dominant antigen epitopes.The immunogenicity of MAP Ⅱ recombinant protein was good.It played an important role in different developmental stages of Echinococcus granulosus,especially in adult stages,and could be used as a vaccine candidate antigen for definitive host of Echinococcus granulosus.

Key words: Echinococcus granulosus; methionine aminopeptidase Ⅱ(MAPⅡ); bioinformatics; prokaryotic expression; transcription level

CLC Number:

S852.73^＋4

CHEN Xuke, PU Na, ZHANG Yuxia, ZHAO Wenqing, ZHAO Jiaxin, HUANG Juncheng, WEI Linying, ZHANG Yanyan, SUN Yan, BO Xinwen, WANG Zhengrong. Bioinformatics Analysis of Methionine Aminopeptidase Ⅱ of Echinococcus granulosus and Its Differential Expression in Different Stages of the Worm[J]. China Animal Husbandry & Veterinary Medicine, 2025, 52(7): 3286-3296.

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Bioinformatics Analysis of Methionine Aminopeptidase Ⅱ of Echinococcus granulosus and Its Differential Expression in Different Stages of the Worm

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