植物乳杆菌硒对蛋鸡卵巢及输卵管组织形态、抗氧化功能和炎症指标的影响

doi:10.16431/j.cnki.1671-7236.2026.02.016

Abstract

Abstract:

Objective This study aimed to investigate the effects of Lactobacillus plantarum selenium on the ovarian and oviduct structure， as well as the antioxidant function and inflammatory indices of laying hens，in order to provide a new reference for selenium sources in maintaining the reproductive health of laying hens. Method A total of 96 healthy 22-week-old Hy-Line Brown laying hens were randomly divided into 2 treatment groups， with 16 replicates per group and 3 hens per replicate. Hens in control group （CON） was fed a conventional corn-soybean meal-based diet for laying hens， with a measured selenium content of 0.38 mg/kg. The Lactobacillus plantarum selenium group （SEL） was supplemented with 500 mg/kg of Lactobacillus plantarum selenium product on the basis of the basal diet， and the measured selenium content in the diet was 1.58 mg/kg. The experiment lasted for 188 days. At the end of the experiment， 8 laying hens were randomly selected from each group for slaughter. Oviduct and ovary tissue samples were collected to analyze the effects of Lactobacillus plantarum selenium on the production performance of laying hens. The tissue structure of ovaries and oviducts was observed by HE staining， and the effect of Lactobacillus plantarum selenium on the ovarian hormone levels of laying hens was further examined. Real-time quantitative PCR was performed to determine the relative expression of antioxidant and inflammation-related genes in ovarian and oviduct. Result ①There were no significant differences in the main production performance indicators such as daily feed intake， egg production rate， average daily egg weight， and feed-egg ratio between the two groups of laying hens （P>0.05）. ② Compared with CON group， the ovarian of SEL group was more intact， and the number of large yellow follicles significantly increased （P<0.05）. Moreover， the levels of follicle-stimulating hormone （FSH） and estradiol （E₂） in ovarian were significantly increased （P<0.05）.③ Compared with CON group， the tissue structure and morphology of the magnum of oviduct in SEL group were more complete. The expression of antioxidant-related genes GPX1， GPX4， CAT， TXNRD2， TXNRD3 and SOD1， as well as inflammatory factor-related genes IL-6 and TNFAIP3 in the magnum tissue of oviduct were significantly increased （P<0.05）. Conclusion The supplementation of feed with Lactobacillus plantarum selenium could improve the morphological structure of ovaries and oviduct tissues in laying hens， and enhance the antioxidant function of oviduct tissues. The results of this experiment indicated that Lactobacillus plantarum selenium has significant application value in improving the health of oviducts and enhancing the overall production efficiency of laying hens.

Key words: laying hens; Lactobacillus plantarum selenium; ovary; oviduct; antioxidant function

CLC Number:

S816

WANG Huixin, GAO Qingtao, LI Jiaheng, ZHANG Shunfen, ZHONG Ruqing, WANG Yang, CHEN Liang, ZHANG Hongfu. Effects of Lactobacillus plantarum Selenium on Tissue Morphology， Antioxidant Function and Inflammatory Indices in Ovary and Oviduct of Laying Hens[J]. China Animal Husbandry & Veterinary Medicine, 2026, 53(2): 682-691.

Figures/Tables 9

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References 51

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原料 Ingredient	含量 Contents	营养成分 Nutrient components^②	含量 Contents
玉米 Corn	64.40	总能 GE/（MJ/kg）	14.36
豆粕 Soybean meal	21.00	干物质 DM	90.81
膨化大豆 Extruded soybean	4.00	粗蛋白质 CP	14.17
石粉 Limestone	8.29	粗脂肪 EE	2.43
磷酸氢钙 Dicalcium phosphate	1.00	粗灰分 Ash	11.95
1%预混料 1% premix^①	1.00	中性洗涤纤维 NDF	7.01
98%赖氨酸 98% lysine	0.10	酸性洗涤纤维 ADF	3.13
DL-蛋氨酸 DL-methionine	0.16	钙 Ca	3.63
L-苏氨酸 L-threonine	0.05	总磷 TP	0.48
总计 Total	100.00

基因 Genes	引物序列 Primer sequences （5′→3′）		登录号 Accession No.	产物长度 Product length/bp
ACTB	F：CTCTATCCTGGCCTCCCT	R：GGGTGTGGGTGTTGGTAA	NM_205518.1	127
IL-10	F：GATGCTGCGCTTCTACACA	R：TGCTTGATGGCTTTGCTC	NM_001004414.2	168
IL-1β	F：TACCGTCCCGTTGCTTTT	R：AATACCTCCACCCCGACA	NM_204524.1	92
IL-6	F：GATAGCGAAAAGCAGAACG	R：CTTCAGATTGGCGAGGAG	NM_204628.1	141
SOD1	F：TTTCTCTTACCGGACCACA	R：CATTTCCCACTGCCATCT	NM_205064.1	194
CAT	F：TGCCTGTTACTTTCCCTCTT	R：TCCAATCCAACTGCTAGTGA	NM_001031215.2	142
TXNRD1	F：TGCCCCATTCCTACGAC	R：CTCCAAGACCCCACGAA	NM_001030762.2	150
TXNRD2	F：AGGGCTTCTCTCCTCTGTC	R：CCTCTTCACTGGCTGTCC	NM_001122691.1	136
TXNRD3	F：GAACGAACCAATGTGCCT	R：GGAGTTTCCCTGCCTGA	NM_001122777.1	94
TNFAIP3	F：CCCTGACTGACCAAGCC	R：CTCCTGACCTCCATGCAC	XM_015284186.1	73
GPX1	F：GAGGTGAACGGGAAGGG	R：TGATGTACTGCGGGTTGG	NM_001277853.1	106
GPX3	F：CGAAAGTACGCGGGGAA	R：AATTGGTTGGAGGGGAAGC	NM_001163232.2	143
GPX4	F：TCGGCCACCTCCATCTAC	R：CGCGGTCTTTCCTCATTT	XM_015299984.1	123

项目 Items	组别 Groups		P值 P-value
项目 Items	CON	SEL	P值 P-value
平均日采食量 ADFI/g	126.26±5.10	128.62±6.72	0.377
产蛋率 LR/%	93.71±3.84	91.98±4.33	0.797
平均日产蛋重 ADE/g	58.11±6.35	59.76±6.32	0.729
料蛋比 Feed-egg ratio	2.20±0.31	2.17±0.20	0.670
软壳蛋率 SR/%	0.68±1.35	0.43±0.84	0.430
破壳蛋率 BER/%	2.70±5.38	1.62±1.86	0.228

项目 Items	组别 Groups		P值 P-value
项目 Items	CON	SEL	P值 P-value
排卵前卵泡 POF	5.75±0.71	5.86±0.69	0.772
小白卵泡 SWF	8.25±2.96	13.71±4.57	0.016
大白卵泡 LWF	12.38±4.78	8.86±2.54	0.106
小黄卵泡 SYF	5.75±2.66	7.00±2.58	0.374
大黄卵泡 LYF	4.63±3.11	5.14±2.85	0.744
卵泡总数 Total number of follicles	36.75±8.94	40.57±10.50	0.460

激素 Hormone	组别 Groups		P值 P-value
激素 Hormone	CON	SEL	P值 P-value
促黄体生成素 LH/（mIU/mL）	4.22±1.66	3.36±2.59	0.463
孕激素 PROG/（ng/mL）	2.69±0.03	2.72±0.02	0.058
促卵泡激素 FSH/（mIU/mL）	1.53±0.01	1.60±0.05	0.001
雌二醇 E₂/（ng/L）	1.84±0.01	1.85±0.01	0.019
抗缪勒管激素 AMH/（ng/L）	0.03±0.01	0.03±0.01	0.664

Effects of Lactobacillus plantarum Selenium on Tissue Morphology， Antioxidant Function and Inflammatory Indices in Ovary and Oviduct of Laying Hens

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基因 Genes	组别 Groups		P值
基因 Genes	CON	SEL	P-value
GPX1	1.06±0.42	0.85±0.36	0.368
GPX3	1.10±0.48	0.91±0.53	0.531
GPX4	1.08±0.41	1.05±0.34	0.915
CAT	1.01±0.19	1.12±0.34	0.533
SOD1	1.00±0.10	1.18±0.32	0.239
TXNRD1	1.03±0.29	0.84±0.50	0.433
TXNRD2	1.19±0.66	1.83±0.91	0.196
TXNRD3	1.16±0.69	1.97±1.17	0.173
IL-1β	0.74±0.33	0.47±0.30	0.196
IL-6	0.78±0.37	0.57±0.54	0.493
IL-10	1.07±0.39	0.84±0.25	0.263
TNFAIP3	1.02±0.22	0.75±0.27	0.084

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基因 Genes	组别 Groups		P值
基因 Genes	CON	SEL	P-value
GPX1	1.07±0.41	2.22±0.73	0.007
GPX3	1.25±0.82	1.82±0.71	0.394
GPX4	1.13±0.57	2.44±0.49	0.009
CAT	1.29±0.99	2.57±0.62	0.023
SOD1	1.13±0.58	2.25±0.39	0.003
TXNRD1	1.02±0.20	1.35±0.48	0.240
TXNRD2	1.34±0.98	2.81±0.61	0.011
TXNRD3	1.29±0.92	2.92±1.08	0.018
IL-1β	1.08±0.45	1.49±0.43	0.132
IL-6	1.09±0.47	1.68±0.34	0.032
IL-10	1.15±0.61	2.12±1.32	0.134
TNFAIP3	1.17±0.69	2.49±0.62	0.006