山香圆叶提取物对脂多糖刺激肉鸡生长性能、免疫、抗氧化功能和肠道健康的影响

doi:10.16431/j.cnki.1671-7236.2025.09.011

Abstract

Abstract: 【Objective】 This study aimed to investigate the effects of Turpiniae folium extract (TFE) on growth performance,immune function,antioxidant capacity,and intestinal health in broilers stimulated with lipopolysaccharides (LPS). 【Method】 A total of 360 one-day-old female broiler chickens were randomly divided into three groups:Control,LPS,and TFE+LPS groups,with 6 replicates of 20 birds each.The trial lasted for 26 days.The chickens in control and LPS groups were fed a basal diet,while in TFE+LPS group were received the same basal diet supplemented with 500 mg/kg TFE.The broilers in LPS and TFE+LPS groups were intraperitoneally injected with 1 mg/kg BW LPS solution at 21,23,and 25 days of age,while the broilers in control group were received an equivalent volume of saline.On day 26 of the trial,growth performance was measured,and two broilers per replicate with body weights closest to the replicate average were selected for blood sampling and slaughter to collect organ tissues and cecal contents.Immune and antioxidant parameters in the serum and liver,jejunum tissue morphology,and cecal microbiota composition were analyzed. 【Result】 Compared to control group,the final body weight,average daily gain (ADG),and average daily feed intake (ADFI) of broilers in LPS group were significantly reduced (P＜0.05).The final body weight,ADG,and ADFI of broilers in TFE+LPS group were significantly higher than those in LPS group (P＜0.05),and they had no significant difference compared with control group (P＞0.05).Compared with control group,LPS stimulation significantly increased the levels of serum soluble CD8 ligand (sCD8),interleukin-6 (IL-6),tumor necrosis factor-α (TNF-α),and malondialdehyde (MDA) in broilers (P＜0.05),while also significantly elevating the hepatic interleukin-1β (IL-1β) content (P＜0.05).Additionally,LPS stimulation significantly reduced serum total antioxidant capacity (T-AOC) and hepatic glutathione peroxidase (GSH-Px) activity compared to control group (P＜0.05).The broilers in TFE+LPS group had significantly lower serum IL-6,TNF-α,MDA,and hepatic IL-1β levels than LPS group (P＜0.05),with no significant difference compared to control group (P＞0.05).LPS stimulation significantly increased the jejunal crypt depth (CD),villus height-to-crypt depth ratio (VH/CD),and the relative abundance of Bacteroidetes and Desulfobacterota in broilers (P＜0.05).The jejunal CD of broilers in TFE+LPS group was significantly higher than that in control group，but significantly lower than that in LPS group (P＜0.05).The Chao1 index,observed species,and relative abundance of Desulfobacterota in the cecum of broilers in TFE+LPS group were significantly higher than those in LPS group (P＜0.05),while the relative abundance of Parabacteroides was significantly lower than that in LPS group (P＜0.05),and there was no significant difference compared to control group (P＞0.05). 【Conclusion】 TFE significantly alleviated LPS-induced declines in immune function and antioxidant capacity, intestinal morphological damage and cecal microbiota disruption in broilers,thereby effectively attenuating the negative effects of LPS on broiler growth performance.

Key words: Turpiniae folium extract; broiler; lipopolysaccharides; immune and antioxidant function; intestinal health

CLC Number:

S831.5

SONG Wenjing, CHEN Jiang, XIONG Pingwen, SONG Qiongli, AI Gaoxiang, WEI Qipeng, ZOU Zhiheng, CHEN Xiaolian. Effects of Turpiniae folium Extract on Growth Performance,Immunity,Antioxidant Capacity and Intestinal Health in Broilers Stimulated by Lipopolysaccharide[J]. China Animal Husbandry & Veterinary Medicine, 2025, 52(9): 4123-4135.

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Effects of Turpiniae folium Extract on Growth Performance,Immunity,Antioxidant Capacity and Intestinal Health in Broilers Stimulated by Lipopolysaccharide

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