四川肉牛源疣状毛癣菌的分离鉴定、系统发育分析与药敏试验

doi:10.16431/j.cnki.1671-7236.2026.01.041

Abstract

Abstract:

Objective This study aimed to explore the etiology of fungal dermatitis in Sichuan cattle and to provide reference for prevention and control of the disease. Method Skin scabs from animals suspected of having dermatophytosis were collected from various locations in Sichuan province， and the collected samples were inoculated into the dermatophyte identification medium by three-point inoculation method. The isolated strains were amplified by internal transcribed spacer （ITS） sequence， and the genetic similarity was analyzed by NCBI BLAST and the phylogenetic tree was constructed. Immunosuppressed mice were infected with 10⁷ CFU/mL spore suspension of the isolates to observe the clinical symptoms and pathological features， and the drug resistance phenotype of the isolates was explored by micro-double dilution method. Result Ten fungal strains （rntv01 to rntv10） were isolated from 24 samples. PCR amplification results showed that all the isolates obtained amplification fragments of 681 bp in size. The BLAST comparison results showed that the similarities between the isolates and Trichophyton verrucosum were 97% to 100%. The results of animal experiments showed that after immunosuppressed mice were infected with the isolates， their skin presented symptoms such as hair loss， scabbing， and increased dandruff. After HE staining， incomplete keratinization of the epidermal stratum corneum and hyperplasia of inflammatory cells were observed， and after PAS staining， a large amount of mycelial infection was observed. The results of the drug sensitivity test showed that， except for rntv10， the other strains were intermediate to amphotericin B. The isolated strains had different sensitivities to itraconazole， rntv09 and rntv10 were sensitive， rntv01 and rntv04-rntv08 were intermediate， and rntv02 and rntv03 were resistant. rntv10 was sensitive to ketoconazole and intermediate to 5-flucytosine， while the remaining strains were drug-resistant. All strains were intermediate to ciclopirox olamine and resistant to fluconazole and terbinafine. Conclusion The main pathogen of fungal dermatitis in Sichuan beef cattle was Trichophyton verrucosum. The fungus caused invasive skin infections in mice. Most of the diseased cattle could be treated with amphotericin B， itraconazole and ciclopirox olamine.

Key words: beef cattle; Trichophyton verrucosum; isolation and identification; ITS sequence; phylogenetic analysis; drug sensitivity test

CLC Number:

S852.66

WANG Ya, LI Zhiguo, LUO Rongyan, LIU Ruiguo, ZUO Zhicai, MA Xiaoping. Isolation， Identification， Phylogenetic Analysis and Drug Sensitivity Test of Trichophyton verrucosum from Beef Cattle in Sichuan[J]. China Animal Husbandry & Veterinary Medicine, 2026, 53(1): 459-468.

Figures/Tables 7

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References 37

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菌株 Strains	最小抑菌浓度 MIC/（μg/mL）
菌株 Strains	两性霉B AMB	环吡酮胺 CPX	伊曲康唑 ITR	酮康唑 KTZ	氟康唑 FCA	5-氟胞嘧啶 5-FC	特比萘芬 TEF
rntv01	8（I）	16（I）	4（I）	256（R）	256（R）	>256（R）	>256（R）
rntv02	8（I）	16（I）	64（R）	256（R）	256（R）	>256（R）	>256（R）
rntv03	8（I）	16（I）	64（R）	64（R）	128（R）	>256（R）	>256（R）
rntv04	8（I）	16（I）	16（I）	64（R）	128（R）	>256（R）	>256（R）
rntv05	8（I）	4（I）	4（I）	128（R）	256（R）	>256（R）	>256（R）
rntv06	8（I）	16（I）	16（I）	64（R）	256（R）	>256（R）	>256（R）
rntv07	4（I）	8（I）	16（I）	64（R）	128（R）	>256（R）	>256（R）
rntv08	8（I）	8（I）	16（I）	128（R）	128（R）	>256（R）	>256（R）
rntv09	8（I）	8（I）	2（S）	64（R）	128（R）	>256（R）	>256（R）
rntv10	64（R）	16（I）	1（S）	2（S）	256（R）	32（I）	64（R）

Isolation， Identification， Phylogenetic Analysis and Drug Sensitivity Test of Trichophyton verrucosum from Beef Cattle in Sichuan

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