鹅胰岛素样生长因子2基因启动子区序列分析

doi:10.16431/j.cnki.1671-7236.2025.10.002

摘要/Abstract

摘要： 【目的】启动子的结构和功能在基因转录调控中至关重要。本研究旨在解析鹅胰岛素样生长因子2(IGF2)基因启动子区的结构特征及其转录调控机制，为揭示IGF2基因在鹅生长发育过程中的作用提供理论依据。【方法】基于鹅IGF2基因5'-侧翼区(GCA_040182565.1) 2 170 bp序列，利用Promoter 2.0、AnimalTFDB 4.0等软件预测其启动子活性区域，分析转录因子结合位点和转录调控元件。使用TBtools软件通过跨物种序列比对分析鹅IGF2基因启动子区的保守性。结合豁眼鹅和浙东白鹅种质间基因组单核苷酸多态性(SNP)分型数据比较不同鹅种质的差异，并对两品种鹅间产蛋期性腺轴组织进行IGF2基因转录表达分析。【结果】鹅IGF2基因启动子活性区域位于1 020～1 932 bp之间，该区域包含CAAT-box、GC-box、TATA-box及与细胞增殖和分化相关的E-box，转录因子Sp1和Irf3可能通过结合这些元件调控基因转录。跨物种序列比对显示，鹅与凤头潜鸭的启动子区高度相似，相似性达92.94%。SNP分型结果显示，豁眼鹅CC基因型比例(89%)明显高于浙东白鹅(25%)，而浙东白鹅CT和TT基因型比例(均为38%)高于豁眼鹅(11%)。在豁眼鹅和浙东白鹅卵巢组织中，IGF2基因的表达差异较为显著(P＜0.05)。【结论】鹅IGF2基因启动子区富含Sp1和Irf3等多种转录因子结合位点，是维持IGF2基因稳定表达的关键调控元件。此外，豁眼鹅和浙东白鹅在IGF2基因启动子区1 450 bp处存在1个SNP位点(C/T)，可通过影响IGF2基因的表达，进而影响鹅的生长发育和产蛋繁殖性能。

关键词: 鹅; IGF2基因; 启动子区; 转录因子; CpG岛

Abstract: 【Objective】 The structure and function of promoters are crucial in gene transcriptional regulation research.This study aimed to analyze the structural characteristics and transcriptional regulatory mechanisms of the insulin-like growth factor 2 (IGF2) gene promoter region in goose,providing a theoretical foundation for understanding the role of IGF2 gene in the growth and development of goose.【Method】 Based on the 2 170 bp sequence of the 5'-flanking region of IGF2 gene (GCA_040182565.1) in goose,the promoter activity region was predicted using tools such as Promoter 2.0,and AnimalTFDB 4.0.The binding sites of transcription factors and regulatory elements were analyzed.The TBtools software was employed to analyze the conservation of the IGF2 gene promoter region in goose through cross-species sequence alignment.Moreover,genomic single nueleotide polymorphism (SNP)genotyping data from Huoyan geese and Zhedong White geese were untlized to compare the genetic variations between both geese breeds.Additionally,the transcriptional expression of IGF2 gene in gonadal axis tissues during the egg-laying period was analyzed for both geese breeds.【Result】 The promoter activity region of IGF2 gene in goose was located 1 020-1932 bp,containing critical elements such as the CAAT-box,GC-box,TATA-box,and E-box,which was associated with cell proliferation and differentiation.The transcription factors Sp1 and Irf3 were identified as potential regulators of gene transcription through binding to these elements.Cross-species sequence alignment revealed a high degree of similarity between the promoter regions of geese and Ferruginous ducks,with a similarity of 92.94%.SNP genotyping analysis showed a significantly higher proportion of CC genotype in Huoyan geese (89%) compared with Zhedong White geese (25%),while the proportions of CT and TT genotypes in Zhedong White geese (both were 38%) were markedly higher than those in Huoyan geese (11%).Furthermore,a significant difference in the expression of IGF2 gene was observed in ovarian tissue between the two goose breeds during the egg-laying period (P＜0.05).【Conclusion】 The promoter region of IGF2 gene in goose was abundant in binding sites for various transcription factors,including Sp1 and Irf3,which played crucial roles in regulating the stable expression of IGF2 gene.Additionally,a SNP variation (C/T) at position 1 450 bp in the promoter region of IGF2 gene had been identified in the germplasms of both Huoyan geese and Zhedong White geese.This genetic variation could affect the growth,development,and egg production performance of geese by influencing the expression of IGF2 gene.

Key words: goose; IGF2 gene; promoter region; transcription factor; CpG island

中图分类号:

S835.2
Q78

赵洪昌, 穆晓惠, 陈冲, 李晓鸣, 王军, 赵孟丽, 郁钧, 谢佳, 张威, 胡团军, 王健. 鹅胰岛素样生长因子2基因启动子区序列分析[J]. 中国畜牧兽医, 2025, 52(10): 4538-4548.

ZHAO Hongchang, MU Xiaohui, CHEN Chong, LI Xiaoming, WANG Jun, ZHAO Mengli, YU Jun, XIE Jia, ZHANG Wei, HU Tuanjun, WANG Jian. Sequence Analysis of Insulin-like Growth Factor 2 Gene Promoter Region in Geese[J]. China Animal Husbandry & Veterinary Medicine, 2025, 52(10): 4538-4548.

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