盐地碱蓬提取物对湖羊瘤胃发酵参数及胃肠道微生物群落结构的影响

doi:10.16431/j.cnki.1671-7236.2026.02.022

Abstract

Abstract:

Objective This experiment aimed to investigate the effects of dietary supplementation with Suaeda salsa extract （SSE） on rumen fermentation parameters and gastrointestinal （ruminal and intestinal） microbial community structure in Hu sheep. Method Thirty-six healthy male Hu sheep lambs with an average body weight of （30.27±1.18） kg were randomly divided into a control group （CON） and an experimental group （SSE）， with 6 replicates per group and 3 lambs per replicate. The sheep in CON group were received a total mixed ration （TMR）， while in SSE group were fed TMR supplemented with 0.6% SSE. The pre-test period lasted for 15 days， followed by a 30-day formal trial period. On the 30th day of the formal trial， rumen fluid and rectal faecal samples were collected from fasted Hu sheep to determine rumen fermentation parameters and ruminal faecal microbial communities. Result ①Compared with CON group， the ruminal total volatile fatty acid （TVFA） content of sheep in SSE group was extremely significantly increased （P<0.01）， and ruminal pH， acetate， propionate， isobutyrate， isovalerate and valerate contents were significantly increased （P<0.05）. ②No significant differences were found in ruminal microbial composition at either the phylum or genus level， nor in Alpha and Beta diversity indices between the two groups （P>0.05）. However， the relative abundance of the genus Enteromonas was significantly higher in SSE group （P<0.05）. Correlation analysis between ruminal microbes and fermentation parameters showed that the relative abundance of Limivicinus was significantly positively correlated with ruminal butyrate concentration （P<0.05）， while the relative abundance of Succiniclasticum was significantly negatively correlated with ruminal butyrate content （P<0.05）.③No significant differences in rectal faecal microbial composition at the phylum level， or in the Alpha and Beta diversity indices was observed between the two groups （P>0.05）. Compared with control group，the relative abundance of Prevotella， Agathobacter， Butyrivibrio_A， Eubacterium_J， and Methylobacterium in the rectum of SSE group were significantly increased （P<0.05）. Conclusion Dietary supplementation with 0.6% SSE to the diet could optimize rumen fermentation parameters， improve the structure and composition of ruminal microbial communities， and increase the genera Agathobacter， Butyricimonas， and Eubacterium in the intestine of Hu sheep.

Key words: Suaeda salsa extract; Hu sheep; rumen fermentation; rumen microorganisms; intestinal microorganisms

CLC Number:

S816.7

LI Changchang, WU Yinglian, YANG Zhenxiang, LI Lingui, QIN Rongyan, LIU Yanfeng, XU Guishan, WANG Wenqi, DIAO Qiyu. Effects of Suaeda salsa Extract on Rumen Fermentation Parameters and Gastrointestinal Microbiota Community Structure of Hu Sheep[J]. China Animal Husbandry & Veterinary Medicine, 2026, 53(2): 749-760.

Figures/Tables 17

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References 28

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常量元素 Major elements	含量 Contents	微量元素 Trace elements	含量 Contents	重金属元素 Heavy metals	含量 Contents
钠Na	48 083.73	铁Fe	316.84	钼Mo	0.94
镁Mg	22 087.77	锌Zn	44.82	钒V	0.75
硫S	21 839.38	铜Cu	14.28	镉Cd	0.46
钾K	10 464.40	铬Cr	8.77	铅Pb	0.24
钙Ca	8 499.61	钴Co	0.41	锡Sn	0.20
磷P	1 993.91	硒Se	0.11	汞Hg	0.00

原料Ingredients	含量Contents	营养成分Nutrient components^②	含量Contents
玉米Corn	35.88	干物质DM	95.10
全麦粉Whole wheat flour	7.62	粗蛋白质CP	12.99
豆粕Soybean meal	3.50	粗脂肪EE	2.10
棉粕 Cottonseed meal	10.20	中性洗涤纤维NDF	25.62
玉米秸秆Corn stalk	30.00	酸性洗涤纤维ADF	15.77
苜蓿Alfalfa	10.00	钙Ca	0.73
预混料Premix^①	1.00	磷P	0.47
磷酸氢钙CaHPO₄	0.80	代谢能ME/（MJ/kg）	10.84
食盐 NaCl	1.00
合计 Total	100.00

项目Items	CON组	SSE组	SEM	P值 P-value
初始体重 IBW/kg	31.23	31.58	0.568	0.535
终末体重 FBW/kg	38.37	38.88	0.265	0.084
pH	6.56^b	6.79^a	0.099	0.029
氨态氮 NH₃-N/（mg/L）	91.72	94.80	4.277	0.486
乙酸 Acetic/（μg/mL）	1 010.71^b	1 140.67^a	42.910	0.027
丙酸 Propionic/（μg/mL）	262.68^b	360.69^a	32.544	0.017
异丁酸 Isobutyric/（μg/mL）	23.25^b	29.25^a	2.473	0.049
丁酸 Butyric/（μg/mL）	332.13	367.82	16.764	0.059
异戊酸 Isovaleric/（μg/mL）	21.21^b	24.57^a	1.483	0.047
戊酸 Valeric/（μg/mL）	21.99^b	29.64^a	2.621	0.015
总挥发性脂肪酸 TVFA/（μg/mL）	1 692.29^B	1 956.05^A	56.949	<0.01
乙丙比 A/P	4.15	3.25	0.655	0.199

项目Items	CON组	SSE组	SEM	P值P-value
Chao1指数 Chao1 index	2 629.64	2 888.13	325.114	0.445
Shannon指数 Shannon index	9.08	8.87	0.458	0.652
Simpson指数 Simpson index	0.99	0.98	0.001	0.440
Goods_coverage指数 Goods_coverage index	0.99	0.99	1.483	0.066

项目Items	CON组	SSE组	SEM	P值 P-value
Chao1指数 Chao1 index	2 566.63	2 685.92	197.131	0.567
Shannon指数 Shannon index	8.62	8.83	0.001	0.826
Simpson指数 Simpson index	0.96	0.99	0.825	0.806
Goods_coverage指数Goods_coverage index	0.99	0.99	0.030	0.436

Effects of Suaeda salsa Extract on Rumen Fermentation Parameters and Gastrointestinal Microbiota Community Structure of Hu Sheep

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