武夷黑猪与杜洛克猪HSPB1基因克隆、生物信息学对比分析及组织表达研究

doi:10.16431/j.cnki.1671-7236.2025.09.023

Abstract

Abstract: 【Objective】 The purpose of this study was to compare and analyze the similarities and differences in the structure and function of heat shock protein beta-1 (HSPB1)between Wuyi Black pig and Duroc pig,and provide a reference for studying animal anti-stress mechanism. 【Method】 The HSPB1 gene of Wuyi Black pig and Duroc pig was cloned and sequenced,and bioinformatics methods were used to compare similarities.A phylogenetic tree was constructed.And a comparative analysis was conducted on the differences in the characteristics,structure,and function of HSPB1 protein between the two pig breeds,as well as the expression of each tissue in the two breeds. 【Result】 The HSPB1 gene was successfully cloned from both Wuyi Black pig and Duroc pig.The coding sequence (CDS) of each gene was 624 bp in length,encoding a protein composed of 207 amino acids.The nucleotide and amino acid sequence similarities between the two breeds were 99.0% and 98.6%,respectively，with variations in nucleotide sites 123^G→T and 233^T→C,as well as differences in amino acids 41^E→D and 78^L→P.The nucleotide sequence evolutionary tree showed that both were closely related to Desmodus rotundus;The evolutionary tree of amino acid sequences showed that both were closely related to Ovis aries.Both had the same gene promoters and transcription factor binding sites,and their HSPB1 proteins displayed consistent features in terms of signal peptides,transmembrane regions,conserved motifs,physicochemical properties,and hydrophilicity/hydrophobicity profiles.Further comparative analysis identified one novel phosphorylation site uniquely recognized by glycogen synthase kinase-3 (GSK-3) and four differential sites potentially involved in interactions with chemical effectors.However,the mitochondrial proportion of HSPB1 protein in Wuyi Black pig was lower than that in Duroc pig.Moreover,seven distinct regions of secondary and tertiary structural divergence were observed between the two proteins.Although both HSPB1 proteins exhibited strong interactions with phosphorylation-related kinases,the Wuyi Black pig had an additional functional annotation related to cytoskeletal organization. HSPB1 gene was broadly expressed across various tissues in both pig breeds,with no significant differences between breeds.In both breeds,the highest expression was observed in the longissimus dorsi muscle. 【Conclusion】 HSPB1 genes of Wuyi Black pig and Duroc pig were successfully amplified.Compared with Duroc pig, HSPB1 in Wuyi Black pig showed higher cytoplasmic content and differences in phosphorylation and chemical factor binding sites.However,the expression was found to be similar across corresponding tissues in both breeds,with the highest expression in longissimus dorsi muscle.The results provided a theoretical foundation for further studying the specific role of porcine HSPB1 in anti-stress response.

Key words: Wuyi Black pig; Duroc pig; HSPB1 gene; bioinformatics; tissue expression

CLC Number:

S828
Q78

LI Zhiquan, GAO Mengruo, WEN Yijun, FU Sijing, XU Shuping, YANG Taotao, ZHANG Zhibang, FANG Xia, LI Kai, LI Pengcheng. Cloning,Bioinformatics Comparative Analysis and Tissue Expression of HSPB1 Gene Between Wuyi Black Pig and Duroc Pig[J]. China Animal Husbandry & Veterinary Medicine, 2025, 52(9): 4248-4259.

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Cloning,Bioinformatics Comparative Analysis and Tissue Expression of HSPB1 Gene Between Wuyi Black Pig and Duroc Pig

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