Abstract: Three breeds of pig (Kele pig, Guizhou White Xiang pig and Qianbei Black pig) were used to constructed DNA pooling, the SNP in exon4 to exon5 of Fsp27 gene were rapidly screened by DNA sequencing. Four SNPs were detected in the three breeds of pig totally, they were intron3-T2169C,exon4-G5A,intron4-G21C and exon5-C5G, the exon4-G5A and exon5-C5G sites led to coding amino acids change from Arginine to Glutarnine, from Threoine to Serine respectively. The results of the bioinformatics analysis indicated that the mutation in loci exon4-G5A could cause the change of mRNA secondary structure, while the exon5-C5G site led to the protein secondary structure change,when the locus was A,G in the exon4-G5A and exon5-C5G respectively the protein tertiary structure would be different from other(G and G,G and C,A and C) base combinations.

Key words: Fsp27 gene; SNPs; bioinformatics; DNA pooling