上海地区牛腹泻相关病毒的检测及分析

doi:10.16431/j.cnki.1671-7236.2026.02.038

Abstract

Abstract:

Objective This study was conducted to understand the epidemic situation of bovine diarrhea-related viruses in Shanghai， and to provide a scientific basis for the prevention and control of bovine diarrhoeal disease. Method RT-PCR method was applied to detect Bovine rotavirus （BRV）， Bovine enterovirus （BEV）， Bovine coronavirus （BCoV） and Bovine viral diarrhea virus （BVDV） from 115 anal swab samples of diarrheal cattle in Shanghai area， and the gene sequences of some positive pathogens were sequenced and analyzed for genetic evolution. Result BRV， BEV and BCoV were detected in 115 anal swab samples from diarrheal cattle， with detection rate of 1.74% （2/115） for each， and the infection types of the detected viruses were all single infections. BVDV was not detected. The genetic evolution analysis results based on BRV VP7 and VP4 genes showed that the VP7 genes of the two detected BRV strains had the highest similarity with the Canadian strain FMV1078090， which was 94.1%， and were closely related to the American Human rotavirus strain 3000015004， both belonged to type G6. The VP4 gene had the highest similarity with Chinese strain HB-3， which was 98.2%， and both belonged to type P［11］. Therefore， the BRV genotypes of the two strains were G6P［11］. The genetic evolution analysis results based on the BEV 5'-UTR gene showed that among the two detected BEV strains， one had the highest similarity to the German strain D8-01， which was 90.6%， and both belonged to type E. The other had the highest similarity to the American strain Wye 8875， which was 93.5%， and both belonged to type F. Moreover， both BEV strains were distantly related to the prevalent strains in China. The results of genetic evolution analysis based on the BCoV N gene showed that the two detected BCoV strains clustered into a large branch with most Chinese and American strains， both belonged to the GⅡb gene group and had a close genetic relationship， while had a distant genetic relationship with the American strain Mebus and the vaccine strain BC94. Conclusion BRV， BEV and BCoV were the main viral pathogens associated with bovine diarrhea in Shanghai， among which the prevalent genotype of BRV was G6P［11］， the epidemiological genotypes of BEV were E and F， and the prevalent genotype of BCoV was GⅡb. This study provided a scientific basis for the formulation of prevention and control strategies for bovine diarrheal disease in Shanghai.

Key words: bovine; diarrhea-related viruses; RT-PCR detection; genetic and evolutionary analysis

CLC Number:

S852.65^＋3

GUI Yaping, XIA Qiqi, YANG Xianchao, LIU Jian, XU Ping, XU Feng, GE Feifei, WANG Jian, ZHAO Hongjin. Detection and Analysis of Bovine Diarrhea-related Viruses in Shanghai[J]. China Animal Husbandry & Veterinary Medicine, 2026, 53(2): 937-947.

Figures/Tables 13

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基因 Genes	引物序列 Primer sequences （5'→3'）	产物长度 Product length/bp	退火温度 Anenaling temperature/℃
BRV VP6	F：CAAGATTGTCGAAGGC	532	55
	R：GAATCCAGCGACCTGA
BEV 5'-UTR	F：CCTTTGTACGCCTGTTTTCCCCACC	497	55
	R：ACACGCCCGGAGGTTAGGATTAGCA
BCoV N	F：CGATCAGTCCGACCAATCTA	597	55
	R：GAGGTAGGGGTTCTGTTGCC
BVDV 5'-UTR	F：GAAGGCCGAAAAGAGGCTA	302	55
	R：CATGTGCCATGTACAGCAGAG
BRV VP7	F：ATGTATGGTATTGAATATACCAC	885	50
	R：AACTTGCCACCATTTTTTCC
BRV VP4	F：TGGCTTCGCTCATTTATAGACA	877	50
	R：ATTTCGGACCATTTATAACC

Detection and Analysis of Bovine Diarrhea-related Viruses in Shanghai

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