包被胍基乙酸对放牧妊娠母羊生产性能、血清生化指标、抗氧化能力及免疫功能的影响

doi:10.16431/j.cnki.1671-7236.2025.12.016

摘要/Abstract

摘要： 【目的】研究补饲包被胍基乙酸(CGAA)对冷季放牧妊娠中后期哈萨克母羊生产性能、血清生化指标、抗氧化能力和免疫功能的影响，为CGAA在反刍动物养殖中的应用提供参考。【方法】选取妊娠第90～100天、体重相近(47.80 kg±0.30 kg)的健康放牧哈萨克母羊60只，随机分为4个处理组，每组15个重复。每只羊每天补饲混有CGAA(对照组0 g、试验Ⅰ组0.6 g、试验Ⅱ组1.2 g和试验Ⅲ组1.8 g)的精料500 g。在补饲条件下饲喂37 d，其中预饲期7 d，试验期30 d。试验期间测定母羊的生产性能(初始体重、终末体重、平均日增重及羔羊初生重)，并从每个处理组中随机选择10只母羊颈静脉采血，测定其血清生化、抗氧化和免疫指标。【结果】试验Ⅱ和Ⅲ组的母羊平均日增重极显著高于对照组和试验Ⅰ组(P＜0.01)；羔羊的初生重和母羊的平均日采食量均显著高于对照组(P＜0.05)。与对照组相比，试验Ⅲ组母羊血清甘油三酯含量显著降低(P＜0.05)；试验Ⅱ组母羊血清尿素氮含量、肿瘤坏死因子含量和谷草转氨酶活性极显著降低(P＜0.01)，肌酐和肌酸含量、超氧化物歧化酶、谷胱甘肽过氧化物酶活性、总抗氧化能力及胰岛素生长因子水平均极显著增加(P＜0.01)，孕酮和皮质醇水平显著降低(P＜0.05)；试验Ⅰ和Ⅱ组母羊血清L-精氨酸和一氧化氮含量均极显著增加(P＜0.01)。【结论】在放牧条件下，补饲CGAA可提高妊娠中后期哈萨克羊母羊的平均日增重和羔羊的初生重；可增强母羊机体抗氧化能力和免疫功能，降低皮质醇等激素的分泌水平。建议妊娠中后期哈萨克羊母羊补饲1.2 g/d CGAA为宜。

关键词: 包被胍基乙酸; 哈萨克羊母羊; 生产性能; 血清生化指标; 抗氧化能力; 免疫功能

Abstract: 【Objective】 The study aimed to investigate the effects of supplementing with cationic guanidine acetic acid (CGAA) on the production performance,serum biochemical indices,antioxidant capacity,and immune function of Kazakh ewes in late gestation during cool-season grazing,to provide a reference for the application of CGAA in ruminant farming. 【Method】 A total of 60 healthy grazing Kazakh ewes with similar body weight (47.80 kg±0.30 kg) on the 90-100th day of gestation were randomly divided into 4 treatment groups with 15 replicates in each group.Each sheep was fed 500 g of concentrate mixed with CGAA (0 g in control group,0.6 g in trial group Ⅰ,1.2 g in trial group Ⅱ and 1.8 g in trial group Ⅲ) every day.Feeding was carried out for 37 days under supplementary feeding conditions,including 7 days for pre-feeding and 30 days for the trial period.During the experiment,the production performance of ewes (initial weight,final weight,average daily gain and lamb birth weight) was measured,and 10 ewes were randomly selected from each treatment group for jugular vein blood collection,and their serum biochemical,antioxidant and immune index were measured. 【Result】 The average daily weight gain of ewes in the experimental groups Ⅱ and Ⅲ were extremely significant higher than that in control group and experimental group Ⅰ (P＜0.01).The birth weight and feed intake of ewes in groups Ⅱ and Ⅲ were significantly higher than those in the control group (P＜0.05).Compared with control group,the serum triglyceride content of ewes in group Ⅲ was significantly reduced (P＜0.05).In group Ⅱ,the content of serum urea nitrogen,tumor necrosis factor and aspartate aminotransferase were extremely significantly decreased (P＜0.01),the contents of creatinine and creatine,the activities of superoxide dismutase and glutathione-peroxidase,total antioxidant capacity and insulin growth factor level were extremely significantly increased (P＜0.01),and progesterone and cortisol hormones were significantly decreased (P＜0.05).The levels of L-arginine and nitric oxide in groupsⅠ and Ⅱ were extremely significantly increased (P＜0.01). 【Conclusion】 Under grazing conditions,supplemental feeding with CGAA could increase the average daily gain of Kazakh sheep ewes and the birth weight of lambs in the middle and late stages of gestation.It could enhance the antioxidant capacity and immune function of ewes,and reduce the secretion level of hormones such as cortisol.It was advisable to feed Kazakh sheep ewes with CGAA 1.2 g/d in the middle and late stages of gestation.

Key words: coated guanidine glycolic acid; Kazakh sheep ewes; production performance; serum biochemical indicators; antioxidant capacity; immune function

中图分类号:

S816.7

李林贵, 杨振祥, 秦荣艳, 刘艳丰, 赵林波, 王乐乐, 王彦, 王文奇. 包被胍基乙酸对放牧妊娠母羊生产性能、血清生化指标、抗氧化能力及免疫功能的影响[J]. 中国畜牧兽医, 2025, 52(12): 5704-5714.

LI Lingui, YANG Zhenxiang, QIN Rongyan, LIU Yanfeng, ZHAO Linbo, WANG Lele, WANG Yan, WANG Wenqi. Effects of Coated Guanidine Glycolic Acid on Production Performance,Serum Biochemical Indicators,Antioxidant Capacity and Immune Function of Grazing Pregnant Ewes[J]. China Animal Husbandry & Veterinary Medicine, 2025, 52(12): 5704-5714.

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