复方中药超微粉对新杨黑羽蛋鸡免疫性能及相关基因表达的影响

doi:10.16431/j.cnki.1671-7236.2025.12.012

摘要/Abstract

摘要： 【目的】试验旨在研究复方中药超微粉对产蛋后期蛋鸡免疫性能的影响。【方法】选取307日龄的新杨黑蛋鸡216只，随机分为3组，每组8个重复，每个重复9只。对照组饲喂基础饲粮，试验组分别在基础饲粮中添加0.5%丹参＋0.25%女贞子＋0.25%蒲公英(复方1)、0.3%益母草＋0.2%丹参＋0.25%女贞子＋0.25%蒲公英(复方2)超微粉。于试验第120天采集血液、输卵管和肝脏组织，测定免疫指标及相关基因表达。【结果】与对照组相比，复方1组蛋鸡输卵管白细胞介素-1β(IL-1β)和肿瘤坏死因子-α(TNF-α)含量显著降低、输卵管γ-干扰素(IFN-γ)的mRNA表达量显著增加(P＜0.05)；复方2组蛋鸡输卵管IL-2、脾脏TNF-α的mRNA表达量显著降低(P＜0.05)；复方1和2组蛋鸡血浆IL-6和TNF-α含量，以及输卵管IL-1β和TNF-α及脾脏IL-1β和核因子-κB(NF-κB)的mRNA表达量均显著降低(P＜0.05)，血浆IL-10含量及输卵管IL-10的mRNA表达量均显著增加(P＜0.05)。【结论】饲粮中添加由益母草、丹参、女贞子和蒲公英组成的复方中药超微粉可通过增加血浆、输卵管和脾脏免疫细胞因子活性、激活输卵管及脾脏相关免疫信号通路来增强产蛋后期蛋鸡的免疫性能，且两个复方对机体免疫性能的调控作用均具有靶标特异性。

关键词: 复方中药超微粉; 新杨黑羽蛋鸡; 免疫性能; 信号通路

Abstract: 【Objective】 This study was conducted to determine the effect of compound Chinese herb ultrafine powder on the immune function of laying hens during late laying period. 【Method】 A total of 216 Xinyang Black-feathered laying hens at 307-day-old were selected and randomly divided into three groups,with eight replicates per group and nine hens per replicate.The birds in control group was fed a basal diet,and the birds in experimental groups were supplemented with 0.5% Salvia miltiorrhiza＋0.25% Ligustrum lucidum＋0.25% Taraxacum (compound 1) and 0.3% motherwort＋0.2% Salvia miltiorrhiza＋0.25% Ligustrum lucidum＋0.25% Taraxacum (compound 2) ultrafine powder,respectively.Samples of plasma，fallopian tube and liver tissue were collected on days 120 of the trial to measure immune ability indexes and related gene expression. 【Result】 Compared with control group,the levels of tubal interleukin-1β (IL-1β) and tumor necrosis factor-α (TNF-α) and the mRNA expression of tubal interferon-γ (IFN-γ) were significantly decreased in compound 1 group (P＜0.05);the mRNA expression of tubal IL-2 and splenic TNF-α was significantly decreased in compound 2 group (P＜0.05). Plasma level of IL-6 and TNF-α and the mRNA expression of tubal IL-1β and TNF-α and splenic IL-1β and NF-κB were significantly decreased (P＜0.05);and plasma content of IL-10 and the mRNA expression of tubal IL-10 were significantly increased in both compound 1 and 2 groups (P＜0.05). 【Conclusion】 Dietary addition with the compound Chinese herb ultrafine powder composed of motherwort,Salvia miltiorrhiza,Ligustrum lucidum and Taraxacum could enhance the immune ability of laying hens during late laying period by increasing plasma,tubal and splenic immune cytokine activity and activating tubal and splenic related immune signaling.In addition,these two compounds presented target-specific in the regulation of the immune function of the body.

Key words: compound Chinese herb ultrafine powder; Xinyang Black-feathered laying hens; immune function; signaling pathway

中图分类号:

S816.7

王远卓, 刘童, 王桂桢, 韩坤良, 郭华. 复方中药超微粉对新杨黑羽蛋鸡免疫性能及相关基因表达的影响[J]. 中国畜牧兽医, 2025, 52(12): 5661-5669.

WANG Yuanzhuo, LIU Tong, WANG Guizhen, HAN Kunliang, GUO Hua. Effects of Compound Chinese Herb Ultrafine Powder on Immune Function and Related Gene Expression of Xinyang Black-feathered Laying Hens[J]. China Animal Husbandry & Veterinary Medicine, 2025, 52(12): 5661-5669.

参考文献

[1] 李红,颜瑶,詹凯,等.新杨黑羽蛋鸡育雏期肠道菌群定植规律分析[J].上海畜牧兽医通讯,2023，4:1-4.
LI H,YAN Y,ZHAN K,et al.Analysis of the colonization pattern of intestinal flora during the brooding period of Xinyang Black-feathered laying hens[J].Shanghai Journal of Animal Husbandry and Veterinary Medicine,2023,4:1-4.(in Chinese)
[2] GUO W,XU L N,GUO X J,et al.The impacts of fermented feed on laying performance,egg quality,immune function,intestinal morphology and microbiota of laying hens in the late laying cycle[J].Animal,2022,16(12):100676.
[3] ZHOU Y M,DONG X R,XU D,et al.Therapeutic potential of traditional Chinese medicine for interstitial lung disease[J].Journal of Ethnopharmacology,2024,318:116952.
[4] 邓必贤,邓文琼,李健,等.黄芪超微粉对三黄鸡小肠绒毛形态结构、肠内容物消化酶活性和盲肠菌群的影响[J].福建农林大学学报：自然科学版,2014,43(6):616-621.
DENG B X,DENG W Q,LI J,et al.Effects of astragalus ultrafine powder on the morphological structure of small intestinal villi,activity of digestive enzymes of intestinal contents and cecal flora of Sanhuang chickens[J].Journal of Fujian Agriculture and Forestry University (Natural Science Edition),2014,43(6):616-621.(in Chinese)
[5] 万妍,许彦飞,董元洋,等.超微粉碎车前对沙门氏菌感染肉仔鸡生长性能,免疫功能和抗氧化功能的影响[J].动物营养学报,2020,32(8):3636-3653.
WAN Y,XU Y F,DONG Y Y,et al.Effects of superfine grinding plantain on growth performance,immune function and antioxidant function of broilers with Salmonella infection [J].Chinese Journal of Animal Nutrition,2020,32(8):3636-3653.(in Chinese)
[6] PENG Y S,LIU J X,JIAO J,et al.Secoiridoid glycosides from the fruits of Ligustrum lucidum and their in vitro anti-inflammatory activity [J].Fitoterapia,2023,171:105705.
[7] LI J J,LIU J X,SHI W B,et al.Role and molecular mechanism of Salvia miltiorrhiza associated with chemical compounds in the treatment of diabetes mellitus and its complications:A review [J].Medicine,2024,103(16):e37844.
[8] 高强,贾绥安,杜宝龙,等.复方中草药对肉鸡生长性能及天然免疫相关基因mRNA表达的影响[J].甘肃农业大学学报,2021,56(1):14-21.
GAO Q,JIA S A,DU B L,et al.Effects of compound Chinese herbal medicine on growth performance of broilers and expression of innate immunity related gene mRNA [J].Journal of Gansu Agricultural University,2021,56(1):14-21.(in Chinese)
[9] LI J H,NIU L H,HUANG H,et al.Anti-inflammatory labdane diterpenoids from the aerial parts of Leonurus sibiricus [J].Phytochemistry,2024,217:113927.
[10] HAO F S,DENG X X,YU X,et al.Taraxacum:A review of ethnopharmacology,phytochemistry and pharmacological activity[J].American Journal of Chinese Medicine,2024,52(1):183-215.
[11] 乔彦杰,杨莉,刘晓婷,等.益母草碱对肉仔鸡生长性能、免疫功能和肠道菌群的影响[J].家畜生态学报,2019,40(5):34-38.
QIAO Y J,YANG L,LIU X T,et al.Effects of Leonurine on growth performance,intestinal flora and immune function in broilers [J].Acta Ecologae Animalis Domastici,2019,40(5):34-38.(in Chinese)
[12] 李赵嘉,孟然,冯薇,等.饲料中添加蒲公英对蛋鸡生产性能,蛋营养品质,血清抗氧化能力及肝脏的影响[J].饲料研究,2022,45(3):48-52.
LI Z J,MENG R,FENG W,et al.Effect of dandelion on growth performance,egg nutritional quality,serum antioxidant capacity and liver of laying hens[J].Feed Research,2022,45(3):48-52.(in Chinese)
[13] 韩坤良,林文超,蒙成文,等.复方中药超微粉对蛋鸡产蛋性能、生殖激素及相关基因表达的影响[J].华南农业大学学报,2023,44(3):374-381.
HAN K L,LIN W C,MENG C W,et al.Effects of compound Chinese medicine ultrafine powder on egg production，reproductive hormones,and related gene expression in laying hens [J].Journal of South China Agricultural University,2023,44(3):374-381.(in Chinese)
[14] 高雨桐,宋秀苹,李楠,等.女贞子-墨旱莲药对提取物的指纹图谱和化学成分鉴定研究[J].南京中医药大学学报,2024,40(4):399-412.
GAO Y T,SONG X P,LI N,et al.Fingerprint and identification of chemical constituents of the extract of fructus Ligustri lucidi-Ecliptae herba [J].Journal of Nanjing University of Traditional Chinese Medicine,2024,40(4):399-412.(in Chinese)
[15] LI Y N,CHEN Y L,SUN-WATERHOUSE D.The potential of dandelion in the fight against gastrointestinal diseases:A review[J].Journal of Ethnopharmacol,2022,293:115272.
[16] HOTAMISLIGIL G S.Inflammation,metaflammation and immunometabolic disorders[J].Nature,2017,542(7640):177-185.
[17] CHEN L,DENG H,CUI H,et al.Inflammatory responses and inflammation-associated diseases in organs[J].Oncotarget,2018,9(6):7204-7218.
[18] NII T,ISOBE N,YOSHIMURA Y.Effects of interleukin-1beta and -6 on the expression of ion transporters involved in eggshell mineralization in cultured hen uterine mucosal tissue[J].Poultry Science,2018,55(2):142-149.
[19] 童小峰,张磊,黄杰,等.淫羊藿苷对蛋鸡生产性能,蛋品质,输卵管氧化还原状态和炎性细胞因子基因表达的影响[J].动物营养学报,2020,32(12):5676-5683.
TONG X F,ZHANG L,HUANG J,et al.Effects of Icariin on performance，egg quality，redox state and gene expression of inflammatory cytokines in oviduct of laying hens[J].Chinese Journal of Animal Nutrition,2020,32(12):5676-5683.(in Chinese)
[20] SHIN H J,YANG W K,LEE Y C,et al.Protective effect of the mixture of Lactiplantibacillus plantarum KC3 and Leonurus Japonicas Houtt extract on respiratory disorders[J].Ecotoxicology and Environmental Safety,2024,270:115856.
[21] DENG X X,JIAO Y N,HAO H F,et al.Taraxacum mongolicum extract inhibited malignant phenotype of triple-negative breast cancer cells in tumor-associated macrophages microenvironment through suppressing IL-10/STAT3/PD-L1 signaling pathways[J].Journal of Ethnopharmacology,2021,274:113978.
[22] 孙露露,王紫媛,葛金山,等.超微粉碎中草药发酵前后活性成分变化及对蛋鸡产蛋性能、蛋品质和血清生化指标的影响[J].饲料工业,2025,46(7):34-42.
SUN L L,WANG Z Y,GE J S,et al.Changes of active ingredients of ultrafine grinding chinese herbal medicine before and after fermentation and their effects on laying performance,egg quality and serum biochemical indicators of laying hens[J].Feed Industry,2025,46(7):34-42.(in Chinese)
[23] KAK G,RAZA M,TIWARI B K.Interferon-gamma (IFN-gamma):Exploring its implications in infectious diseases [J]. BioMolecular Concepts,2018,9(1):64-79.
[24] 侯道荣,刘振,崔斯童,等.丹参酮II-A通过调控TLR4/IκBα/NFκB信号通路抑制LPS诱导的细胞炎症[J].中国药理学通报,2021,37(2):210-214.
HOU D R,LIU Z,CUI S T,et al.Tanshinone II-A inhibits LPS induced inflammation of RAW264.7 cells by regulating TLR4/IκB-α/NF-κB signaling pathway[J].Chinese Pharmacological Bulletin,2021,37(2):210-214.(in Chinese)
[25] 熊佳伟,马睿琦,于华鹏,等.益母草碱在炎症相关性疾病中作用的研究进展[J].复旦学报(医学版),2024,51(4):614-619.
XIONG J W,MA R Q,YU H P,et al.Research progress of the use of leonurine in inflammatory related diseases [J].Fudan University Journal of Medical Sciences,2024,51(4):614-619.(in Chinese)
[26] 王海波,秦士贞,李金录,等.低锌饲粮添加载锌蒙脱石对肉鸡免疫功能和肝脏含锌酶活性的影响[J].动物营养学报,2021,33(8):4779-4790.
WANG H B,QIN S Z,LI J L,et al.Effects of adding zinc-montmorillonite in low zinc diet on immune function and zinc-containing enzyme activities in liver of broilers[J].Chinese Journal of Animal Nutrition,2021,33(8):4779-4790.(in Chinese)
[27] 李鹏,夏平安,宋咏梅,等.鸡TLR4基因表达水平与沙门氏菌感染关系的研究[J].中国畜牧兽医,2009,36(5):72-74.
LI P,XIA P A,SONG Y M,et al.Study on the relationship between chicken Toll like receptor 4 expression level and Salmonella infection[J].China Animal Husbandry & Veterinary Medicine,2009,36(5):72-74.(in Chinese)
[28] 甘建伉,张泽樘,罗庆斌.鸡沙门氏菌抗性基因研究进展[J].中国家禽,2012,34(11):48-50.
GAN J K,ZHANG Z T,LUO Q B,et al.Progress in the study of resistance genes of Salmonella spp in chickens [J].China Poultry,2012,34(11):48-50.(in Chinese)
[29] 郭斯璇,李冰心,许丹宁,等.TLR4基因在三黄鸡不同器官中的表达规律分析[J].中国家禽,2019,41(11):7-11.
GUO S X,LI B X,XU D N,et al.Expression and analysis of TLR4 gene in different organs of Sanhuang chicken [J].China Poultry,2019,44(11):7-11.(in Chinese)
[30] REN Y,CUI G,GAO Y.Research progress on inflammatory mechanism of primary sjogren syndrome[J].Journal of Zhejiang University.Medical Sciences,2021,50(6):783-794.
[31] YANG N,LI C,TIAN G,et al.Organic acid component from Taraxacum mongolicum Hand.-Mazz alleviates inflammatory injury in lipopolysaccharide-induced acute tracheobronchitis of ICR mice through TLR4/NF-kappaB signaling pathway[J].International Immunopharmacology,2016,34:92-100.
[32] HONG Z Y,SHI X R,ZHU K,et al.SCM-198 inhibits microglial overactivation and attenuates Abeta(1-40)-induced cognitive impairments in rats via JNK and NF-small ka,CyrillicB pathways[J].Journal of Neuroinflammation,2014,11:147.
[33] SHANG X,PAN H,WANG X,et al.Leonurus japonicus Houtt.:Ethnopharmacology,phytochemistry and pharmacology of an important traditional Chinese medicine[J].Journal of Ethnopharmacology,2014,152(1):14-32.
[34] XIAO C J,LIU Y C,LUO S H,et al.Localisation of two bioactive labdane diterpenoids in the peltate glandular trichomes of Leonurus japonicus by laser microdissection coupled with UPLC-MS/MS[J].Phytochemical Analysis,2017,28(5):404-409.
[35] YANG K,WANG Y H.Dandelion root extracts and taraxasterol inhibit LPS-cinduced colorectal cancer cell viability by blocking TLR4-NFκB-driven ACE2 and TMPRSS2 pathways[J].Experimental and Therapeutic Medicine,2024,27(6):256.
[36] LI P,WANG Y H,YAN Q Q,et al.Fructus ligustri Lucidi inhibits ferroptosis in ovariectomy-induced osteoporosis in rats via the Nrf2/HO-1 signaling pathway[J].Biomedical Reports,2023,20(2):27.
[37] 李瑞,张雯,宋俊科,等.注射用丹参多酚酸通过PI3K/Akt和JAK2/STAT3信号通路减轻OGD/R导致PC12细胞损伤[J].中国药学杂志,2023,58(5):419-425.
LI R,ZHANG W,SONG J K,et al.Salvianolic acids for injection reduces the damage of PC12 cells caused by OGD/R by inhibiting apoptosis and affecting PI3K/Akt and JAK2/STAT3 signaling pathways[J].Chinese Pharmaceutical Journal,2023,58(5):419-425.(in Chinese)