基于转录组测序筛选浙籽鹅F2代繁殖性状相关候选基因

doi:10.16431/j.cnki.1671-7236.2025.11.022

摘要/Abstract

摘要： 【目的】以浙东白鹅(♂)与浙籽鹅F1代(♀)杂交产生的浙籽鹅F2代母鹅为研究对象，分析浙籽鹅F2代产蛋期和休产期血液转录组数据，筛选浙籽鹅F2代繁殖性状相关的候选基因，为杂交鹅的繁殖调控提供参考。【方法】采集浙籽鹅F2代产蛋期和休产期血液样本，利用Illumina NovaSeq 6000测序平台进行转录组测序(RNA-Seq)分析。利用DESeq筛选差异表达基因(DEGs)，对其进行GO功能和KEGG通路富集分析，筛选与繁殖性状相关的基因。随机挑选6个差异表达基因进行实时荧光定量PCR验证。【结果】转录组测序结果显示，在产蛋期与休产期两个比较组中存在差异表达的基因4 576个，其中上调基因1 821个，下调基因2 755个。GO功能富集分析显示，共富集到9 643个GO条目，在生物过程中主要富集在钙离子跨膜转运的正调节、吞噬作用的调节、阳离子跨膜转运的正向调节等条目；在分子功能上主要富集在转移酶活性、催化活性、嘌呤核苷三磷酸结合等条目；在细胞组分中主要富集在细胞内。KEGG通路富集分析显示，共富集到157条通路，主要富集在果糖和甘露糖代谢、烟酸盐和烟酰胺代谢、氨基糖和核苷酸糖代谢等通路，筛选出ADCY5、CD44、CCND1是与繁殖性状相关的候选基因。实时荧光定量PCR结果与转录组测序趋势相符合，证实转录组测序结果可靠。【结论】本研究通过转录组测序筛选到3个(ADCY5、CD44、CCND1)与繁殖性状相关的候选基因，为研究浙籽鹅F2代繁殖性状相关基因的功能及后续分子调控机制提供依据。

关键词: 浙籽鹅F2代; 转录组; 信号通路; 候选基因

Abstract: 【Objective】 This study focused on the F2 generation female Zhezi geese derived from the crossbreeding of male Zhejiang White geese (♂) and female F1 generation Zhezi geese(♀),aiming to identify candidate genes related to reproductive traits through transcriptome analysis of blood samples collected during the laying and ceased periods,providing a reference for reproductive regulation in hybrid geese. 【Method】 Blood samples were collected from F2 generation Zhezi geese during the laying and ceased periods,and transcriptome sequencing (RNA-Seq) was performed using the Illumina NovaSeq 6000 sequencing platform.Differentially expressed genes (DEGs) were identified using DESeq,followed by GO function and KEGG pathway enrichment analysis to screen for genes associated with reproductive traits.Six differentially expressed genes were randomly selected for validation using Real-time quantitative PCR. 【Result】 Transcriptome sequencing results revealed a total of 4 576 differentially expressed genes between the laying and ceased periods,including 1 821 upregulated genes and 2 755 downregulated genes.GO function enrichment analysis identified 9 643 GO terms,primarily enriched in biological process such as positive regulation of calcium ion transmembrane transport,regulation of phagocytosis,and positive regulation of cation transmembrane transport,enriched in molecular functions including transferase activity,catalytic activity,purine nucleoside triphosphate binding,etc.,and enriched in cellular components mainly within intracellular.KEGG pathway enrichment analysis identified 157 pathways,mainly enriched in fructose and mannose metabolism,nicotinate and nicotinamide metabolism,and amino sugar and nucleotide sugar metabolism,with ADCY5,CD44,and CCND1 identified as candidate genes related to reproductive traits.Real-time quantitative PCR results were consistent with the transcriptome sequencing trends,confirming the reliability of the sequencing data. 【Conclusion】 This study identified three candidate genes (ADCY5,CD44,and CCND1) associated with reproductive traits through transcriptome sequencing,providing a foundation for functional research and subsequent molecular regulatory mechanisms in F2 generation Zhezi geese.

Key words: F2 generation Zhezi geese; transcriptome; signal pathway; candidate gene

中图分类号:

S835.3

岳山, 赵秀华, 孙金艳, 彭福刚, 张元良, 李满雨, 刘国君. 基于转录组测序筛选浙籽鹅F2代繁殖性状相关候选基因[J]. 中国畜牧兽医, 2025, 52(11): 5254-5264.

YUE Shan, ZHAO Xiuhua, SUN Jinyan, PENG Fugang, ZHANG Yuanliang, LI Manyu, LIU Guojun. Screening of Candidate Genes Related to Reproductive Traits in F2 Generation Zhezi Geese Based on Transcriptome Sequence[J]. China Animal Husbandry & Veterinary Medicine, 2025, 52(11): 5254-5264.

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