2021—2023年江苏省部分地区鹅源禽致病性大肠杆菌的分离鉴定及生物学特性分析

doi:10.16431/j.cnki.1671-7236.2025.10.043

摘要/Abstract

摘要： 【目的】了解鹅源禽致病性大肠杆菌(APEC)的分子流行特点及耐药性，为水禽大肠杆菌病的预防与控制提供参考。【方法】从江苏省不同地区采集病鹅病料240份，采用平板划线法分离纯化细菌，通过16S rDNA PCR扩增鉴定阳性菌株，并检测分离株O抗原血清型、系统进化分群和毒力基因携带情况，通过药敏试验检测其耐药情况，并进行多重耐药分析。【结果】从240份病料中分离获得31株APEC分离株。血清型检测结果显示，O145血清型检出率最高，为19.35%，其次为O1(6.45%)、O78(6.45%)和O2(3.23%)血清型。系统进化分群分析结果显示，分离株属于A、B1、B2和D进化分群的比例分别为38.71%、22.58%、9.68%和29.03%。毒力基因检测结果显示，fimA和hlyF基因检出率均＞90%，iroN、ompT、iucD、ECs3737、ECs3703基因检出率为60%～90%，iss、tsh、irp2、cvaC、papC和hlyE基因检出率＜50%，eaeA基因未检出，且所有分离株均至少含有4个毒力基因。药敏试验结果显示，分离株对强力霉素、林可霉素、氨苄青霉素和阿莫西林的耐药率均＞80%，对新霉素、氟苯尼考、恩诺沙星等5种抗菌药的耐药率达50%～70%，而大观霉素(74.19%)和庆大霉素(54.84%)敏感率较高，14重耐药的菌株占比最高(19.35%)，7重以上耐药菌株占64.52%。【结论】江苏省鹅源APEC分离株血清型复杂，携带多种毒力基因，多重耐药严重。研究结果为该地区水禽大肠杆菌病的防治提供了数据支撑。

关键词: 禽致病性大肠杆菌; 血清型; 进化分群; 毒力基因; 耐药

Abstract: 【Objective】 The aim of this study was to understand the molecular epidemiological characteristics and drug resistance of avian pathogenic Escherichia coli (APEC) from geese,so as to provide reference for the prevention and control of colibacillosis in waterfowl.【Method】 A total of 240 diseased goose samples were collected from different regions in Jiangsu province.The strains were isolated and purified using the plate streaking method.Positive strains were identified through 16S rDNA PCR amplification，and O antigen serotype,phylogenetic cluster and virulence gene carrying of the isolates were detected.Drug resistance was detected by drug sensitivity test,and multi-drug resistance analysis was performed.【Result】 A total of 31 strains of APEC were isolated from 240 clinical samples.The serotype detection results showed that the detection rate of O145 serotype was 19.35%,followed by O1 (6.45%),O78 (6.45%) and O2 (3.23%) serotypes. Phylogenetic cluster analysis showed that the proportion of the isolates belonging to A,B1,B2,and D evolutionary groups were 38.71%,22.58%,9.68%,and 29.03%,respectively.Virulence gene detection results showed that the detection rates of fimA and hlyF genes were both above 90%,the detection rates of iroN,ompT,iucD,ECs3737,ECs3703 genes were 60%-90%,the detection rates of iss,tsh,irp2,cvaC,papC and hlyE genes were below 50%，and eaeA gene was not detected.All isolates contained at least 4 virulence genes.The drug susceptibility test results showed the resistance rates of the isolates to doxycycline,lincomycin,ampicillin and amoxicillin were more than 80%,and the resistance rates of the isolates to neomycin,flufenicol and enoxacin were 50%-70%,while the sensitivity rates of the isolates to daviomycin (74.19%) and gentamicin (54.84%) were higher.The proportion of 14 drug-resistant strains was the highest (19.35%),and the proportion of more than 7 drug-resistant strains was 64.52%.【Conclusion】 The results indicated that the serotype of the APEC isolates from geese in Jiangsu province was complex,carrying multiple virulence genes,and exhibiting severe multidrug resistance.The results provided data support for the prevention and control of colibacillosis in waterfowl in this area.

Key words: avian pathogenic Escherichia coli; serotype; phylogenetic group; virulence gene; drug resistance

中图分类号:

S852.6

郭长明, 袁橙, 封琦, 杨静静, 朱善元, 于圣青. 2021—2023年江苏省部分地区鹅源禽致病性大肠杆菌的分离鉴定及生物学特性分析[J]. 中国畜牧兽医, 2025, 52(10): 4989-4999.

GUO Changming, YUAN Cheng, FENG Qi, YANG Jingjing, ZHU Shanyuan, YU Shengqing. Isolation,Identification,and Biological Characteristics Analysis of Avian Pathogenic Escherichia coli from Geese in Some Areas of Jiangsu Province from 2021 to 2023[J]. China Animal Husbandry & Veterinary Medicine, 2025, 52(10): 4989-4999.

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