›› 2010, Vol. 37 ›› Issue (9): 194-196.

• 疾病防治 • 上一篇    下一篇

鸡源乳杆菌的分离鉴定及其益生特性研究

宁豫昌，张晓静，潘春梅   

  1. （郑州牧业工程高等专科学校，郑州 450011）
  • 收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2010-09-20 发布日期:2010-09-20

Isolation and Identification of Lactobacillus from Chicken and Study on its Probiotic Characteristics

NING Yu-chang, ZHANG Xiao-jing, PAN Chun-mei   

  1. (Zhengzhou College of Animal Husbandry Engineering, Zhengzhou 450011, China)
  • Received:1900-01-01 Revised:1900-01-01 Online:2010-09-20 Published:2010-09-20

摘要： 利用选择性培养、革兰氏染色、芽孢染色、糖发酵试验、经典生理生化试验、耐酸和耐胆盐试验等方法，成功地从健康鸡的肠道中分离、鉴定并筛选出1株耐酸和耐胆盐能力较强的乳杆菌，命名为乳杆菌L3株。采用雏鸡安全性试验和肠黏液糖蛋白黏附性试验对乳杆菌L3株的安全性和黏附能力进行检测，证明所筛选的乳杆菌不仅对酸和胆盐的耐受性较好，且具有良好的安全性和肠道黏附能力。

关键词: 乳杆菌; 鸡; 分离; 鉴定; 益生特性

Abstract: Through selective culture, Gram staining, spore staining, sugar fermentation tests, the classical physiological and biochemical testing, acid resisting test, bile salt resisting test, bacteriostatic test in vitro and so on, a Lactobacillus with strong tolerance of acid and bile salt from the healthy chickens was successfully isolated, identified and screened out, and named as Lactobacillus strains L3. The safety and adhesion of Lactobacillus strains L3 were detected by chicken safety test and intestinal mucus glycoprotein adhesion test.The results showed that the Lactobacillus tolerated acid and bile salt, and had a good safety and capacity of intestinal adhesion.

Key words: Lactobacillus; chicken; isolation; identification; probiotic characteristics

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