China Animal Husbandry & Veterinary Medicine ›› 2025, Vol. 52 ›› Issue (11): 5276-5286.doi: 10.16431/j.cnki.1671-7236.2025.11.024

• Genetics and Breeding • Previous Articles    

Cloning and Bioinformatics Analysis of FGF2 Gene in Guangxi Cattle and Its Expression Pattern in Ovarian Follicles

WANG Yun1, ZHENG Yanzi2, TANG Zhenhua1,3, WANG Yanxin1, JIA Ruru1, WEI Chunye1, ZHOU Dongping1, LU Ying3, HUANG Rongchun3, SHI Deshun1, LU Fenghua1   

  1. 1. Guangxi Key Laboratory of Animal Breeding, Disease Control and Prevention, College of Animal Science and Technology, Guangxi University, Nanning 530000, China;
    2. Qinzhou City Animal Disease Prevention and Control Center, Qinzhou 535000, China;
    3. Guangxi Buffalo Research Institute, Nanning 530000, China
  • Revised:2025-06-18 Published:2025-10-30

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Abstract: 【Objective】 This study aimed to clone the coding sequence (CDS) of the fibroblast growth factor 2 (FGF2) gene in Guangxi cattle and conduct bioinformatics analysis,investigate the expression patterns of FGF2 gene in various tissues and ovarian follicles,and establish a foundation for further exploration of its functional roles in follicular development. 【Method】 The FGF2 gene fragment was amplified from Guangxi cattle ovarian cDNA,followed by sequence alignment and phylogenetic tree construction with other species.Bioinformatics analysis of FGF2 protein was performed using online tools.The expression of FGF2 gene in different tissues of Guangxi cattle were detected via Real-time quantitative PCR.ELISA was employed to quantify FGF2 concentrations in follicular fluid from small (3-4 mm diameter),medium (5-8 mm),and large (＞8 mm) follicles.Immunofluorescence and Real-time quantitative PCR were combined to analyze the spatial localization and expression dynamics of FGF2 gene in follicular cells. 【Result】 The CDS of FGF2 gene in Guangxi cattle spanned 468 bp,encoding 155 amino acids.The amino acid sequence similarity of FGF2 protein in Guangxi cattle exhibited ＞98% with Sus scrofa,Capra hircus,Ovis aries,and Oryctolagus cuniculus,demonstrating high conservation among mammals.Bioinformatics analysis results revealed that FGF2 was an alkaline hydrophilic protein containing 14 phosphorylation sites,with secondary structures dominated by random coil (61.94%) and extended chain (29.03%),and key interacting partners included FGFR,PDGFRA,GPC1,and EGFR.Tissue expression analysis revealed that FGF2 gene was expressed in various tissues of Guangxi cattle,with significantly higher expression in ovarian and lung tissues compared with other tissues (P＜0.05).Follicular fluid FGF2 concentrations in large follicles (＞8 mm) were significantly elevated relative to small follicles (3-4 mm) (P＜0.05).The expression of FGF2 gene in oocytes was significantly higher than that in granulosa and theca cells (P＜0.05),while its receptor FGFR1 gene expression in oocytes was significantly lower than that in granulosa and theca cells (P＜0.05). 【Conclusion】 The CDS region of FGF2 gene in Guangxi cattle was 468 bp,encoding a polypeptide of 155 amino acids characterized as a stable alkaline hydrophilic protein,with its secondary structure dominated by random coil.FGF2 gene exhibited significantly higher expression in ovarian and lung.Within ovarian follicles,its expression was predominantly localized to oocytes,and it regulated follicular development via follicle diameter-dependent concentration dynamics.The results provided a theoretical foundation for elucidating the functional regulatory network of FGF2 gene in ovaries of Guangxi cattle.

Key words: Guangxi cattle; FGF 2 gene; cloning; bioinformatics; expression pattern; follicle development

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