人兽共患寄生虫病候选疫苗分子筛选方法研究进展

doi:10.16431/j.cnki.1671-7236.2021.06.031

Abstract

Abstract: There are many kinds of zoonotic parasites with a wide range of hosts and serious damage.Schistosomiasis,echinococcosis,cysticercosis,trichinosis and toxoplasmosis are common parasitic zoonoses.Humans and livestock suffer from parasitic diseases,which have a significant impact on public health and livestock farming.Controlling the source of infection,cutting off the route of transmission and protecting susceptible groups are the comprehensive prevention and control measures to control the epidemic of parasitic zoonoses.In particular,vaccinations is one of the ideal and effective ways to cut off parasites’ life cycle,and control and even eliminate parasitic zoonoses.It is the premise and key to develop a vaccine to select an effective antigen screening method to excavate the potential candidate molecules for vaccine.With the development of antigen screening technology,more new antigens and protective peptides have been discovered.Existing antigen screening methods mainly include the traditional crude antigen screening method,cDNA library screening method,proteomics screening method,bioinformatics and multi-omics technology screening method.Many methods of antigen screening were developed along with the process of parasite vaccine research.The crude antigen screening method is based on the immunological principle of antigen-antibody interaction,and natural antigen screened by this method can cause a strong immune response.The advantage of cDNA library screening is that the screening is more targeted,so the composition of the candidate product is more single and clear.Proteomic screening technique is based on mass spectrometry,which can not only identify the unknown proteins,but also compare the differences of the identification results.It plays an important role in the discovery of unknown molecules and the screening of target molecules with special functions.With the advent of the post-genetic era,the combined screening technology of bioinformatics and multi-omics makes antigen screening turn into a multi-dimensional screening mode,and also makes the functional studies of candidate antigens and their epitopes more in-depth,which provides a technical means for the screening of candidate molecules of genetically engineered vaccines and polypeptide vaccines.

Key words: parasite; vaccine; antigen screening; cDNA library; proteomics; bioinformatics; multi-omics

CLC Number:

S855.9

DAI Guodong, YAN Hongbin, LI Li, FU Baoquan, JIA Wanzhong. Research Progress on Screening Methods for Candidate Vaccine Molecules Against Parasitic Zoonoses[J]. China Animal Husbandry & Veterinary Medicine, 2021, 48(6): 2177-2187.

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Research Progress on Screening Methods for Candidate Vaccine Molecules Against Parasitic Zoonoses

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