China Animal Husbandry & Veterinary Medicine ›› 2007, Vol. 34 ›› Issue (6): 109-111.

• 疾病防治 • Previous Articles     Next Articles

Isolation and Identitication of PRV SA Strain and Construction of gE-delection Transfer Vector

GUAN Yu1,2,SHEN Zhiqiang2,QU Guanggang2,LV Sufang2, REN Yanling2,LI Shuguang2, WANG Yujiong1
   

  1. 1.Ningxia University Life Sciences College, Yinchuan 750021,China;2.Shandong Binzhou Animal Science and Veterinary Medichine Academy, Binzhou 256600,China
  • Received:1900-01-01 Revised:1900-01-01 Online:2007-06-20 Published:2007-06-20

Abstract: The brain Sample of swine that had been doubtfully infected Aujeszky's disease was obtained .The sample was treated and inoculated into Vero and the cell indicated CPE after 7 generations.PCR analysis showed the virus was PRV.The virus was inoculated into other cells and the BHK-21 cell was the optimization for the virus with TCID50 10-9.25/0.1 ml .The PM gene fragment that contains YFP express frame,and the PL and PR fragment used for recombination that were cloned from PRV gE gene,were sequentially inserted into pBluescript(M13-) vector.The results of identitication showed that the gE-delection transfer vector had been successfully constructed.

Key words: PRV; isolation and identitication; gE; transfer vector

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