杜洛克公猪精液性状的遗传参数估计

doi:10.16431/j.cnki.1671-7236.2025.07.026

Abstract

Abstract: 【Objective】 This study aimed to investigate the effects of different fixed effects on semen traits,estimate the genetic parameters of semen traits in Duroc boars,and evaluate the genetic and phenotypic correlations among semen traits,providing a reference for the selection and production management of breeding boars. 【Method】 Semen-related traits of Duroc boars were assessed using a computer-assisted sperm analysis (CASA) system and flow cytometry.ANOVA and significance tests were conducted to examine the fixed effects on semen traits using R software,including semen collection method,semen collection season,and semen collection month.The genetic parameters of semen traits were estimated using single-trait and multi-trait animal models,applying the PBLUP method in ASReml software. 【Result】 The fixed effects of semen collection method,semen collection season,and semen collection month had varying impacts on each semen trait.The single-trait animal model estimation revealed that most semen traits in Duroc boars had low heritability,except semen density,which had medium heritability.Heritability estimates for each trait ranged from 0.11 (mitochondrial membrane potential) to 0.29 (semen density).Multi-trait animal model estimation showed that the heritability estimates for each trait ranged from 0.10 (abnormal sperm percentage) to 0.30 (semen density).In terms of genetic correlations,a strong negative correlation was observed between reactive oxygen species (ROS) level and acrosome integrity rate (r=-0.570),and between ROS level and mitochondrial membrane potential (r=-0.730).A strong positive correlation was found between semen density and sperm motility (r=0.860),as well as between mitochondrial membrane potential and acrosome integrity rate (r=0.930).In terms of phenotypic correlation,there was a moderate correlation between semen density and sperm motility (r=0.320),as well as between mitochondrial membrane potential and acrosome integrity rate (r=0.380). 【Conclusion】 Each fixed effect affected semen traits in Duroc boars,which were low to medium heritability,and there was a genetic correlation among semen traits,which could be selected to improve the semen quality of boars.

Key words: Duroc boars; semen traits; flow cytometry; genetic parameter estimation

CLC Number:

S814

NIE Jingkun, ZHANG Yaxuan, YANG Xiyan, WANG Suqing, ZHU Xiaoping, ZHAO Yunxiang. Estimation of Genetic Parameters of Semen Traits in Duroc Boars[J]. China Animal Husbandry & Veterinary Medicine, 2025, 52(7): 3256-3263.

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Estimation of Genetic Parameters of Semen Traits in Duroc Boars

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