China Animal Husbandry & Veterinary Medicine ›› 2011, Vol. 38 ›› Issue (8): 60-62.

• 生物技术 • Previous Articles     Next Articles

Analysis of Parts of Pig DAZ Gene by DNA Pooling and Sequencing

LI Jing-rui1, DING Yuan-hua2, XIANG Cheng-ju3, LIU Ruo-yu1   

  1. 1. Key Laboratory of Animal Genetics, Breeding and Reproduction in the Plateau Mountainous Region, Ministry of Education, College of Animal Sciences, Guizhou University, Guiyang 550025,China;2. Breeding Stock Farm, Region of Bijie, Bijie 551700, China;3. Technical Extending Stations of Animal Husbandry, Region of Bijie, Bijie 551700, China
  • Received:2011-01-17 Revised:1900-01-01 Online:2011-08-20 Published:2011-08-20

Abstract: Three breeds of male pigs (Coke pig, Yorkshire and White pig) were applied to sequence in parts of CDS,3'UTR of DAZ gene based on DNA pooling and PCR products. The results showed that DAZ gene were found mutations in the three pig breeds. The results of homologous analysis showed that the homologous rate of nucleotide sequences of the parts of CDS, 3'UTR in human, orangutan, monkey, mouse and cow were 97.0%,95.2%,96.1%,92.9% and 97.0%,respectively. It showed that the parts of CDS and 3'UTR of the DAZ gene sequence in mammals were highly conservaton. Pigs could be used to establish animal mode, providing scientific foundation. Phylogenetic analysis showed that pig and cow were in a branch on the evolutionary. The analysis of the protein hydrophobicity showed that a strong hydrophobic was in the 17 to 20.

Key words: DAZ gene; DNA pooling; sequencing; analysis

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