犬瘟热病毒贵州分离株N基因的克隆及序列分析

Abstract

Abstract: Two pairs of primers were designed and synthesized based on the sequence of the Onderstepoort strain of canine distemper virus reported in GenBank,and the N gene was amplified by reverse transcription polymerase chain reaction(RT-PCR) from CDV-GZ1 strain in Guizhou.The amplified fragment was cloned and analyzed.The results showed that the length of N gene was 1572 bp.The homology of coding amino acid between this strain and CDV Shuskiy and 01-2689 strain was 98.9% and 97.1% respectively,while above 95% among other domestic strain.It illustrated that N protein have highly conservation property.

Key words: canine distemper virus; N protein; clone; sequence analysis

CLC Number:

ZHOU Li;LIU Zhi-jie;ZENG Zhi-yong;TANG De-yuan;LI Qian;WANG Bin;ZHANG Xiao-jie;GAN Zhen-lei;WANG Feng. Cloning and Sequence Analysis of N Gene of Canine Distemper Virus Isolated from Guizhou[J]. China Animal Husbandry & Veterinary Medicine, 2011, 38(8): 63-66.

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Cloning and Sequence Analysis of N Gene of Canine Distemper Virus Isolated from Guizhou

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