China Animal Husbandry & Veterinary Medicine ›› 2010, Vol. 37 ›› Issue (3): 78-83.

• 生物技术 • Previous Articles     Next Articles

The Dynamics of IFN-β mRNA Expression in Chick Embryo Fibroblast Infected with H5N1 Avian Influenza Virusand Virulent Newcastle Disease Virus

GAO Yan1,2, YANG Song-pei3， LEI Xiao-wen1,2, YUE Hua1,2, LI Ming-yi4, TANG Cheng1,2   

  1. (1.College of Life Science and Technology, Southwest University for Nationalities, Chengdu 610041, China; 2.Sichuan Province Key Laboratory of Veterinary Medicine, Chengdu 610041, China; 3.Sichuan Province Institute of Veterinary Drugs Control,Chengdu 610041, China； 4.China Animal and Epidemiology Center, Qingdao 266032, China )
  • Received:1900-01-01 Revised:1900-01-01 Online:2010-03-20 Published:2010-03-20
  • Contact: TANG Cheng

Abstract: According to the chicken’s IFN-β （ChIFN-β） gene sequences, a pair of primers was designed for developing a SYBR Green Ｉ quantitative real-time reverse-transcription PCR (RRT-PCR) to detect the IFN-β gene of chicken, and the expression levels of IFN-β mRNA in chick embryo fibroblast (CEF) at 3, 6, 12, 24 and 30 h after infected with H5N1 avian influenza virus (AIV) and virulent Newcastle disease virus (vNDV) were detected. The results showed that the assay was specific for ChIFN-β gene with amplification efficiency of 94.18%, and the linear range was 10-8 to 10-3 with good correlation coefficient of 0.992, as well as the detection limit reached to 48 copies per reaction. The expression of IFN-β mRNA in CEF infected with AIV H5N1 and vNDV showed, the expression of IFN-β mRNA in CEF was suppressed significantly at 6 and 12 h, then induced at 24 h and significantly induced at the 30 h after infected with AIV H5N1; the IFN-β mRNA expression of CEF was suppressed significantly at 24 h and induced significantly at 30 h after infected with vNDV. The results provided valuable information for further researching the interaction of H5N1/NDV and organism.

Key words: interferon-β; real-time RT-PCR; avian influenza virus; Newcastle disease virus; chick embryo fibroblast

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