icaB基因对耐甲氧西林金黄色葡萄球菌N315株生物被膜及耐药性的影响

doi:10.16431/j.cnki.1671-7236.2026.01.039

Abstract

Abstract:

Objective This study aimed to explore the effect of icaB gene on biofilm-formation ability and antimicrobial resistance of methicillin-resistant Staphylococcus aureus （MRSA）. Method MRSA N315 standard strain was selected to construct icaB gene knockout strain N315ΔicaB by homologous recombination technology， and the differences between strain N315ΔicaB and wild strain N315 WT in terms of growth tendency， colony morphology， cell wall thickness， biofilm-formation ability and antimicrobial resistance were comparatively analysed. Result There was no difference in the growth trend， colony shape and color in N315ΔicaB compared to strain N315 WT. The results of cell wall thickness measurement showed that there was no significant difference in cell wall thickness in strain N315ΔicaB compared to strain N315 WT （P>0.05）. The results of biofilm-formation ability showed that there was no significant difference in biofilm-formation ability between strains N315ΔicaB and N315 WT （P>0.05）. The antimicrobial resistance results showed that the minimum inhibitory concentrations of penicillin， oxacillin， gentamicin， ciprofloxacin and nitrofurantoin against strain N315ΔicaB were lower than those in strain N315 WT. Conclusion The knockout of icaB gene did not affect the growth status and biofilm-formation ability of MRSA. However， it increased its susceptibility to several antimicrobials. This study successfully constructed icaB gene knockout strain N315ΔicaB， which provided an ideal cell model to explore the new functions of icaB gene， and laid a foundation for in-depth study of biofilm regulatory networks at the genetic level. Meanwhile， it also provided a scientific basis for enhancing the efficacy of antibacterial drugs against MRSA by targeting the icaB gene.

Key words: methicillin-resistant Staphylococcus aureus; gene knockout; biofilm; minimum inhibitory concentration

CLC Number:

S852.61

JIN Bingtian, DU Pengcheng, YANG Xinyu, JIER Sanye, YANG Feng, DONG Pengcheng. Effect of icaB Gene on Biofilm and Drug Resistance of Methicillin-resistant Staphylococcus aureus Strain N315[J]. China Animal Husbandry & Veterinary Medicine, 2026, 53(1): 439-447.

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抗菌药物 Antimicrobials	MIC判读标准 MIC interpretive criteria			MIC
抗菌药物 Antimicrobials	S	I	R	N315 WT	N315ΔicaB
青霉素 PEN	≤0.12	－	≥0.25	12	8
苯唑西林 OXA	≤2	－	≥4	128	96
头孢西丁 FOX	≤4	－	≥8	64	64
庆大霉 GEN	≤4	8	≥16	1	0.5
氯霉素 CHL	≤8	16	≥32	40	40
四环素 TET	≤4	8	≥16	0.5	0.5
环丙沙星 CIP	≤1	2	≥4	0.75	0.5
万古霉素 VAN	≤2	4~8	≥16	0.38	0.38
利奈唑胺 LZD	≤4	－	≥8	6	6
呋喃妥因 NIT	≤32	64	≥128	24	16
甲氨苄啶-磺胺甲噁唑 COT	≤2/38	－	≥4/76	0.094	0.094
红霉素 ERM	≤0.5	1~4	≥8	>256	>256

Effect of icaB Gene on Biofilm and Drug Resistance of Methicillin-resistant Staphylococcus aureus Strain N315

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