甘露消毒丹对猫传染性腹膜炎病毒复制及细胞自噬功能的影响

doi:10.16431/j.cnki.1671-7236.2025.11.045

Abstract

Abstract: 【Objective】 Feline infectious peritonitis (FIP) was caused by Feline infectious peritonitis virus (FIPV),which seriously endangered the health and life of cats.At present,there were no safe and effective antiviral drugs for the treatment of this disease.The aim of this study was to investigate the effect of Ganlu Xiaodu micropills on virus replication after FIPV infection of host cells,in order to reveal the mechanism of Chinese medicine treatment and accumulate data for the treatment of FIP. 【Method】 Western blotting was used to detect the expression of autophagy related proteins LC3,Beclin-1 and p62 in the intestinal tissues of cats with FIP.FIPV strain B5 was inoculated into CRFK cells in vitro to observe its effect on cell morphology and growth status.The concentration of Ganlu Xiaodu micropills,which had no toxic and side effects on the growth of CRFK cells,was screened by cytotoxicity test.At this concentration,the intracellular viral load was detected by Real-time quantitative PCR.Western blotting was used to verify the effect of Ganlu Xiaodu micropills on protein LC3，and the MAPK pathway upstream of autophagy related proteins MEK,ERK and mTOR during virus replication. 【Result】 Compared with control group,in the intestinal tissues of cats with FIP,FIPV could significantly reduce the expression levels of LC3,Beclin-1 and p62,as well as the LC3-Ⅱ/LC3-Ⅰ ratio (P＜0.05).In CRFK cells,FIPV infection induced cell shedding and fusion.Compared with virus group,80 μg/mL Ganlu Xiaodu micropills could significantly reduce the intracellular viral load,increase the ratio of LC3-Ⅱ/LC3-Ⅰ,downregulate the expression of MEK protein,and upregulate the expressions of EPK and mTOR proteins (P<0.05). 【Conclusion】 Ganlu Xiaodu micropills could inhibit virus replication by regulating MAPK pathway,improving the level of autophagy and eliminating FIPV,reversing the reduction of host immunity caused by FIPV infection.

Key words: Ganlu Xiaodu micropills; Feline infectious peritonitis virus (FIPV); autophagy; MAPK pathway

CLC Number:

YIN Guibin, GUO Haotong, ZHAO Wenhao, SONG Jian, YANG Yutian, LI Jia, LIU Xiaoye, CHEN Wu. Effect of Ganlu Xiaodu Micropills on Feline Infectious Peritonitis Virus Replication and Cellular Autophagic Function[J]. China Animal Husbandry & Veterinary Medicine, 2025, 52(11): 5510-5519.

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Effect of Ganlu Xiaodu Micropills on Feline Infectious Peritonitis Virus Replication and Cellular Autophagic Function

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