黄淮肉羊IGF1基因序列特征、组织表达及其与产肉性状的相关性分析

doi:10.16431/j.cnki.1671-7236.2024.12.025

Abstract

Abstract: 【Objective】 This study was aimed to research the sequence characteristics and tissue expression patterns of insulin like growth factor 1 (IGF1) gene in Huanghuai sheep,and conduct correlation analysis between the expression of IGF1 gene in longissimus dorsi muscle and meat production traits.【Method】 The 9 male Huanghuai sheep at the ages of 3,9,and 18 months each were selected.The meat production performance were determined and semitendinosus,quadriceps femoris,triceps brachii,longissimus dorsi muscle,subcutaneous fat,rumen,kidney,spleen,liver,testis and small intestine tissue samples were collected after slaughter.the IGF1 gene of Huanghuai sheep was cloned and sequenced，and analyzed by bioinformatics analysis.The mRNA levels of IGF1 gene in various tissues at 9-month-old and in the longissimus dorsi muscle at different age stages were detected by Real-time quantitative PCR,and the correlation between IGF1 expression and meat production traits were analyzed through Pearson correlation coefficient.【Result】 The CDS region size of IGF1 gene in Huanghuai sheep was 465 bp,encoding 154 amino acids,indicating the closest genetic relationship with Capra hircus.IGF1 protein of Huanghuai sheep was a hydrophilic and unstable protein with no transmembrane structure.It contained signal peptides and had 29 potential phosphorylation sites.The secondary structure of IGF1 protein in Huanghuai sheep mainly contained random coil (54.55%) and alpha helix (29.87%)，and the predicted results of the tertiary structure were consistent with the secondary structure.There were interactions between IGF1 and IGF1R,IGFBP3,IGF2 and other proteins.The results of Real-time quantitative PCR showed that IGF1 gene was widely expressed in the tissues of Huanghuai sheep,with the highest expression in liver and the lowest expression in triceps brachii.The expression level of IGF1 gene was highest in the longissimus dorsi muscle of 3-month-old Huanghuai sheep,significantly higher than that of 9-month-old and 18-month-old sheep (P＜0.05),while the expression of 9-month-old sheep was significantly lower than that of 18-month-old sheep (P＜0.05).The correlation analysis results showed that the expression of IGF1 gene was not significantly correlated with growth traits and meat quality indicators (P＞0.05).There was a significant or extremely significant positive correlation between body height,body length,chest girth,cannon circumference,body weight,and carcass weight (P＜0.05 or P＜0.01).There was also a significant or extremely significant positive correlation between dressing percentage,meat color,marble texture,and loin muscle area (P＜0.05 or P＜0.01).【Conclusion】 The CDS sequence of IGF1 gene in Huanghuai sheep was successfully cloned.The IGF1 protein was the hydrophilic and unstable alkaline protein,mainly expressed in the liver,and the general trend in the longissimus dorsi muscle muscle were first decreasing and then rising with age increasing,and was not significantly correlated with growth and development and meat quality indicators.These results provided a reference for further exploring the role of IGF1 gene in muscle development and meat quality regulation in Huanghuai sheep.

Key words: Huanghuai sheep; IGF1 gene; tissue expression; correlation

CLC Number:

S826

LI Jun, SONG Mengke, GAO Fuxian, HAN Wanli, HAN Haoyuan, SHI Huibin, QUAN Kai. Sequence Characteristics,Tissue Expression of IGF1 Gene and Correlation with Production Trait in Huanghuai Sheep[J]. China Animal Husbandry & Veterinary Medicine, 2024, 51(12): 5380-5391.

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Sequence Characteristics,Tissue Expression of IGF1 Gene and Correlation with Production Trait in Huanghuai Sheep

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