茯苓多糖对LPS诱导猫肾细胞炎症的保护作用研究

doi:10.16431/j.cnki.1671-7236.2023.11.041

Abstract

Abstract: 【Objective】 This study was aimed to investigate the protective effect of Poria cocos polysaccharide (PCP)on lipopolysaccharide (LPS) induced inflammation in crandell rees feline kidney (CRFK) and its anti-inflammatory mechanism.【Method】 MTT method was used to detect the changes of cell activity by different concentrations of PCP (5, 10, 15, 25, 35 and 45 μg/mL) and LPS (20, 40, 60, 80, 100, 125 and 150 μg/mL) on CRFK.The LPS-induced CRFK inflammation model was treated with different concentrations of PCP to observe the morphological changes of cells.Nitric oxide (NO) release and cell apoptosis in CRFK were detected by nitric reductase method and flow cytometry, respectively.Real-time quantitative PCR and Western blotting were used to detect the expression levels of genes and proteins related to cell inflammation and apoptosis.【Result】 5-25 μg/mL PCP was non-toxic to CRFK and could reverse the cell morphological changes of CRFK after LPS stimulation.25 μg/mL PCP significantly decreased NO release and apoptosis rate in CRFK after LPS stimulation (P<0.05).Real-time quantitative PCR results showed that different concentrations of PCP could significantly reduce the mRNA expressions of IL-6, TNF-α, Caspase3, Caspase8, Caspase9, Bid and Bax in LPS-induced CRFK, and significantly up-regulate the mRNA expression of Bcl-2 (P<0.05).Western blotting results showed that the expression levels of TLR4, NF-κB, Caspase3 and Caspase9 proteins in LPS-induced CRFK were significantly increased (P<0.05), but after PCP treatment, the expression levels of TLR4, NF-κB, Caspase3 and Caspase9 proteins were significantly decreased (P<0.05).【Conclusion】 PCP could inhibit LPS-induced cell inflammatory damage of CRFK by regulating apoptosis, and its mechanism might be related to the inhibition of TLR4-NF-κB signaling pathway.The results provided a new target and experimental basis for the treatment of CRFK inflammation by PCP.

Key words: Poria cocos polysaccharide; crandell reese feline kidney; lipopolysaccharide; inflammatory; apoptosis

CLC Number:

S859.7

LIU Jiali, LIN Bing, LIU Xin, YAN Pupu, XIA Jinjin, HUANG Yongxi, BAI Ruonan, GUO Liwei, LIU Guoping. Protective Effect of Poria cocos Polysaccharides on LPS-induced CRFK Cell Inflammation[J]. China Animal Husbandry & Veterinary Medicine, 2023, 50(11): 4747-4758.

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Protective Effect of Poria cocos Polysaccharides on LPS-induced CRFK Cell Inflammation

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