基于祖源分析的猪遗传成分鉴定

doi:10.16431/j.cnki.1671-7236.2020.02.026

Abstract

Abstract: It is great reference value to analyze traceability the genetic components of different brands of pork for the protection of commercial brands,traceability and the improvement and development of local pig breeds in China.Four kinds of pig muscle tissues from different sources were selected from the market and the whole genome was resequenced..Combined with the published data of 59 pigs,the ancestral components were analyzed to speculate the possible sources of their ancestors and the contents of their ancestral components.In addition,mitochondrial genomic data were used to determine the maternal origin of four pigs and reveal the possible maternal origin.The 59 reference individuals included 12 breeds,Duroc,Landrace,Largewhite,Pietrain,Erhualian,Jinhua,Jiangquhai,Meishan,Bamaxiang,Luchaun,Wuzhishan and Xiang pigs respectively.The 12 breeds were divided into commercial pig,Eastern pig and Southern pig.In the study,four individuals were evaluated for their ancestral components using the software of ADMIXTURE and RFMix.The results showed that one of the four tested individuals contained 54.8% of commercial pig ingredients and 45.2% of Southern China pig ingredients,the other contained 81.3% of commercial pig ingredients,while the remaining two contained more than 97.0% of commercial pig ingredients.When the ancestral group was set as 12 groups,it was found that one of them contained 64.8% Duroc and 35.2% Luchuan pig;the other contained more complex ancestral components,including 22.5% Landrace,27.6% Large White,8.6% Wuzhishan,5.5% Erhualian,Meishan,Jiangquhai and Bama Xiang;the remaining were about 1.2%.The ancestors of the remaining two pigs were mainly Duroc,Large White and Pietran.In addition,the progeny judgment of mitochondria suggested that the maternal origin of one pig might be from Luchuan or similar local breeds.Through the commonly used method of ancestral analysis,the ancestral components of four pigs were estimated,and find that the genetic components of four pigs were different,and the ancestral components and proportion of different individuals were found.The results showed that the genetic component sources of each test individual could be estimated by the method of ancestral analysis.Using ancestral components as genetic characteristics could provided important genetic reference for brand pork identification and brand protection,as well as guidance and reference for the protection and development of local pig breeds in China.

Key words: pig; SNP; mtDNA; population structure; genetic component

CLC Number:

BU Lina, PENG Yebo, ZHANG Chunyuan, LIU Yangxiu, ZHAO Yiqiang. A Ancestry Analysis of Genetic Components of Pigs[J]. China Animal Husbandry & Veterinary Medicine, 2020, 47(2): 544-553.

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A Ancestry Analysis of Genetic Components of Pigs

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