猪δ冠状病毒对断奶仔猪肠道细胞外基质及其动态变化的影响

doi:10.16431/j.cnki.1671-7236.2025.07.030

Abstract

Abstract: 【Objective】 The purpose of this study was to investigate the effect of Porcine deltacoronavirus (PDCoV) on the intestinal extracellular matrix (ECM) and its dynamic regulators in weaned piglets,in order to alleviate the infection of porcine pathogens and improve the intestinal health of piglets. 【Method】 Sixteen healthy 8-day-old piglets (Duroc×Landerace×Yorkshire) were selected for the experiment and randomly divided into control and PDCoV groups,8 piglets in each group with the control group receiving 20 mL of DMEM by oral gavage and the PDCoV group receiving 20 mL of PDCoV cell culture solution (10⁸ TCID₅₀/mL) by oral gavage,and another administration was given at an interval of 8 h.All piglets were slaughtered on the third day after challenge,and the middle jejunum and distal ileum samples were collected for the assay of ECM-related indices such as intestinal collagen,fibronectin (FN),integrin (ITG) and their related regulatory factors. 【Result】 Compared with control group,the mRNA expressions of interleukin-1β (IL-1β),IL-6 and IL-8 gene in jejunum of piglets in PDCoV group were significantly increased (P＜0.05).Masson staining showed that PDCoV increased jejunal collagen deposition.Compared with control group,the mRNA expressions of FN1 gene in jejunum and ITGA1 gene in ileum were significantly reduced in PDCoV group (P＜0.05).In jejunum,the mRNA expressions of ECM regulators matrix metalloproteinase-2 (MMP2),MMP9,plasminogen activator (PAs),transport and Golgi tissue 2 (TANGO2) gene in PDCoV group were significantly lower than those in control group (P＜0.05),and the mRNA expressions of endoplasmic reticulum channel protein (SEC6A1) and phosphodiesterase 4δ (PDE4D) gene were significantly higher than those in control group (P＜0.05).Compared with control group,the mRNA expressions of MMP2,PAs and SEC6A1 gene in ileum of piglets in PDCoV group were significantly decreased (P＜0.05),and the mRNA expression of MMP9 gene was significantly increased (P＜0.05).As shown by Spearman correlation analysis,the regulatory factors MMP2, TANGO2 and PAs gene were significantly negatively correlated with proinflammatory factors IL-1β and IL-8,and were significantly positively correlated with TGF-β gene (P＜0.05).SEC6A1 and PDE4D gene were significantly positive correlation with IL-1,IL-6 and IL-8 gene (P＜0.05),and PDE4D gene was significantly negative correlation with TGF-β gene (P＜0.05). 【Conclusion】 PDCoV increased the deposition of collagens by regulating the expression of ECM regulatory factors and decreasing the expression of fibronectin and integrins,leading to the dynamic changes of intestinal ECM and disrupting the intestinal homeostasis in weaned piglets.

Key words: Porcine deltacoronavirus (PDCoV); weaned piglets; extracellular matrix; matrix metalloproteinases

CLC Number:

S852.65^＋1

RUAN Shihui, LIU Chunyan, WEI Yangyang, HE Yiyi, WU Qiwen, XIONG Yunxia, YANG Xuefen, WANG Li, YI Hongbo. Effect of PDCoV on the Extracellular Matrix of the Intestine and Its Dynamic Changes in Weaned Piglets[J]. China Animal Husbandry & Veterinary Medicine, 2025, 52(7): 3297-3307.

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Effect of PDCoV on the Extracellular Matrix of the Intestine and Its Dynamic Changes in Weaned Piglets

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