小檗碱联合抗菌药对动物源大肠杆菌的体外抑菌效果研究

doi:10.16431/j.cnki.1671-7236.2025.07.045

摘要/Abstract

摘要： 【目的】探究小檗碱与抗菌药联用对动物源大肠杆菌的体外抑菌作用效果。【方法】试验采用微量肉汤稀释法分别测定小檗碱及抗菌药(恩诺沙星、环丙沙星、多西环素、氟苯尼考、黏菌素)对18株动物源大肠杆菌的最小抑菌浓度(MIC)；通过棋盘稀释法测定小檗碱与抗菌药联合作用的分级抑菌浓度指数(FICI)；针对FICI≤0.50的药物联用组合，测定时间-杀菌曲线分析其抑菌动力学特点。【结果】 18株大肠杆菌对恩诺沙星、环丙沙星、多西环素、氟苯尼考、黏菌素的耐药率分别为55.56%、55.56%、50.00%、88.89%和27.78%；小檗碱与恩诺沙星联用的FICI为0.31～2.00，协同率为33.33%；小檗碱与环丙沙星联用的FICI为0.31～2.00，协同率为27.78%；小檗碱与多西环素联用的FICI为0.50～1.00，协同率为27.78%；小檗碱与氟苯尼考联用的FICI为0.63～0.75，协同率为0；小檗碱与黏菌素联用的FICI为0.31～1.00，协同率为66.67%。时间-杀菌曲线显示，1/2 MIC小檗碱与1/2 MIC黏菌素联合作用于菌株E-01和E-06、1/2 MIC小檗碱与1/2 MIC恩诺沙星联合作用于菌株E-06、1/2 MIC小檗碱与1/2 MIC多西环素联合作用于菌株E-03，24 h内菌液浓度比单药作用时降低≥2 lg CFU/mL，呈现明显的协同作用。【结论】小檗碱与抗菌药联用对多数大肠杆菌表现出不同程度的协同或相加作用，试验结果为兽医临床小檗碱与抗菌药联用增强抗菌药的作用效果研究提供了参考。

关键词: 小檗碱; 抗菌药; 最小抑菌浓度(MIC); 分级抑制浓度指数(FICI)

Abstract: 【Objective】 The purpose of this experiment was to investigate the in vitro antibacterial effect of berberine combined with antimicrobial drugs on Escherichia coli of animal origin. 【Method】 The minimum inhibitory concentration (MIC) of berberine and antimicrobial drugs (enrofloxacin,ciprofloxacin,doxycycline,flufenicol and colistin) against 18 strains of Escherichia coli of animal origin were determined by microbroth dilution method.The fractional inhibitory concentration index (FICI) of the combination of berberine and antimicrobial drugs was determined by checkerboard dilution method.For FICI≤0.50 drug combination,the time-bactericidal curve was determined to analyze its bacteriostatic dynamics. 【Result】 The resistance rates of 18 strains of Escherichia coli to enrofloxacin,ciprofloxacin,doxycycline,florfenicol,and colistin was 55.56%,55.56%,50.00%,88.89% and 27.78%,respectively.The FICI of berberine combined with enrofloxacin ranged from 0.31 to 2.00,and the synergistic rate was 33.33%.The FICI of berberine combined with ciprofloxacin ranged from 0.31 to 2.00,and the synergistic rate was 27.78%.The FICI of berberine combined with doxycycline ranged from 0.50 to 1.00,and the synergistic rate was 27.78%.The FICI of berberine combined with florfenicol ranged from 0.63 to 0.75,and the synergistic rate was 0.The FICI of berberine combined with colistin ranged from 0.31 to 1.00,and the synergistic rate was 66.67%.The time-sterilization curve showed that 1/2 MIC berberine combined with 1/2 MIC colistin against strains E01 and E06,1/2 MIC berberine combined with 1/2 MIC enrofloxacin against strain E-05,and 1/2 MIC berberine combined with 1/2 MIC doxycycline against strain E-03,the concentration of bacterial solution decreased by more than 2 lg CFU/mL compared with the monotherapy effect at 24 h,showing obvious synergistic effect. 【Conclusion】 The combination of berberine and antimicrobial drugs exhibited varying degrees of synergistic or additive effects on most strains of Escherichia coli,which provided a reference for enhancing the efficacy of antimicrobial drugs in veterinary clinical practice.

Key words: berberine; antimicrobial drugs; minimal inhibit concentration (MIC); fractional inhibitory concentration index (FICI)

中图分类号:

S859.7

黄慧, 罗梦东, 娄飞, 祁宇彤, 裴亚玲, 王晓琳, 彭志锋, 李利红. 小檗碱联合抗菌药对动物源大肠杆菌的体外抑菌效果研究[J]. 中国畜牧兽医, 2025, 52(7): 3471-3478.

HUANG Hui, LUO Mengdong, LOU Fei, QI Yutong, PEI Yaling, WANG Xiaolin, PENG Zhifeng, LI Lihong. In vitro Antibacterial Effect of Berberine Combined with Antimicrobial Drugs on Escherichia coli of Animal Origin[J]. China Animal Husbandry & Veterinary Medicine, 2025, 52(7): 3471-3478.

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