FABP4基因在妊娠前后小尾寒羊乳腺中表达差异分析

doi:10.16431/j.cnki.1671-7236.2025.07.024

摘要/Abstract

摘要： 【目的】脂肪酸结合蛋白(fatty acid binding protein,FABP)是一种重要的功能蛋白质，在调节脂质代谢方面发挥关键作用。特别是FABP4被认为是哺乳动物细胞中主要负责脂肪酸转运的载体蛋白，其在机体脂质的合成与分解过程中发挥着至关重要的调节作用。探究FABP4基因在绵羊乳腺中的表达规律对于揭示该基因调控乳脂形成具有重要意义，可为后续研究绵羊泌乳提供理论基础。【方法】选取空怀期和妊娠120 d小尾寒羊的乳腺、乳头组织作为研究对象，通过苏木精-伊红染色法(HE)观察妊娠前后乳腺、乳头组织的结构；采用免疫组织化学观察FABP4蛋白在妊娠前后乳腺、乳头组织中的分布；采用实时荧光定量PCR和Western blotting方法分别检测妊娠前后乳腺、乳头组织中FABP4基因和蛋白的表达水平。【结果】 FABP4基因在空怀期和妊娠期小尾寒羊乳腺、乳头组织中均呈阳性表达。空怀期小尾寒羊乳腺和乳头中FABP4基因表达量显著或极显著高于妊娠期(P＜0.05；P＜0.01)；空怀期乳头中FABP4基因表达量显著高于乳腺组织(P＜0.05)。空怀期小尾寒羊乳腺和乳头中FABP4蛋白表达量极显著高于妊娠期(P＜0.01)；空怀期和妊娠期乳头中FABP4蛋白表达量显著高于乳腺组织(P＜0.05)。【结论】 FABP4基因在妊娠期小尾寒羊乳腺、乳头组织中表达量显著降低，提示该基因在乳腺发育中发挥了重要作用，参与了乳腺周期性的发育。

关键词: FABP4基因; 脂肪酸; 小尾寒羊; 妊娠; 乳腺

Abstract: 【Objective】 Fatty acid binding protein (FABP) is an important functional protein that plays a key role in regulating lipid metabolism.Especially,FABP4 is considered the main carrier protein responsible for fatty acid transport in mammalian cells,playing a crucial regulatory role in the synthesis and decomposition of lipids in the body.Therefore,exploring the expression patterns of FABP4 gene in sheep mammary glands will be significant in revealing the importance of this gene in regulating milk fat formation,providing a theoretical basis for subsequent research on sheep lactation. 【Method】 The mammary gland and nipple tissues of Small-tailed Han sheep during the empty period and at 120 days of gestation were selected as research subjects,and the structure of the mammary gland and nipple tissues before and after pregnancy were observed using the hematoxylin-eosin staining method (HE).The distribution of FABP4 protein in mammary gland and nipple tissues before and after pregnancy was observed using immunohistochemistry,and the expression of FABP4 gene and protein in mammary gland and nipple tissues before and after pregnancy were detected using Real-time quantitative PCR and Western blotting,respectively. 【Result】 FABP4 gene was positively expressed in mammary gland and nipple tissues of Small-tailed Han sheep during the empty period and pregnancy.The expression of FABP4 gene in mammary gland and nipple of Small-tailed Han sheep during the empty period was significantly or extremely significantly higher than that during the pregnancy (P＜0.05 or P＜0.01).The expression of FABP4 gene in nipple during the empty period was extremely significantly higher than that in mammary gland (P＜0.01).The expression of FABP4 protein in mammary gland and nipple of Small-tailed Han sheep during the empty period was extremely significantly higher than that during the pregnancy (P＜0.01).The expression of FABP4 protein in nipple during the empty period and pregnancy was significantly higher than that in mammary gland (P＜0.05). 【Conclusion】 The expression of FABP4 gene in mammary gland and nipple tissues of Small-tailed Han sheep during pregnancy was significantly reduced,indicating that this gene played an important role in breast development and was involved in the periodic development of breast.

Key words: FABP4 gene; fatty acid; Small-tailed Han sheep; pregnancy; mammary gland

中图分类号:

S826.3

蔡文静, 慈秋阳, 唐麟, 柳杭, 孙鑫铭, 陈洋, 姜怀志. FABP4基因在妊娠前后小尾寒羊乳腺中表达差异分析[J]. 中国畜牧兽医, 2025, 52(7): 3234-3241.

CAI Wenjing, CI Qiuyang, TANG Lin, LIU Hang, SUN Xinming, CHEN Yang, JIANG Huaizhi. Differential Expression Analysis of FABP4 Gene in Mammary Gland of Small-tailed Han Sheep Before and After Pregnancy[J]. China Animal Husbandry & Veterinary Medicine, 2025, 52(7): 3234-3241.

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