4-萜烯醇对大肠杆菌的抗菌作用研究

doi:10.16431/j.cnki.1671-7236.2025.09.044

摘要/Abstract

摘要： 【目的】评价4-萜烯醇的抗菌活性，为其结构优化改造和临床应用提供支撑。【方法】本试验以大肠杆菌为模式菌,通过倍比稀释法测定4-萜烯醇对大肠杆菌的最小抑菌浓度(MIC)；通过测定D_{600 nm}值绘制其抑菌曲线；扫描电子显微镜观察4-萜烯醇对大肠杆菌形态的影响。测定胞外碱性磷酸酶(ALP)和β-半乳糖苷酶的含量评价4-萜烯醇对大肠杆菌细胞膜的影响；使用超微量分光光度计检测大肠杆菌核酸和蛋白质的泄漏情况；用核酸凝胶电泳检测4-萜烯醇对大肠杆菌DNA的影响。【结果】 4-萜烯醇对大肠杆菌的MIC为0.013 mol/L,其对大肠杆菌的抑制作用呈浓度依赖性，1 MIC的4-萜烯醇能在24 h内完全抑制大肠杆菌增殖。经4-萜烯醇处理后的大肠杆菌菌体肿胀变形、表面粗糙、菌体间黏连。与对照组相比，0.5 MIC的4-萜烯醇能导致大肠杆菌上清中ALP含量显著升高(P＜0.05)，β-半乳糖苷酶含量极显著升高(P＜0.01)，且与4-萜烯醇浓度呈正相关；0.5 MIC的4-萜烯醇即可导致大肠杆菌上清中蛋白质和核酸含量增高，且与4-萜烯醇浓度和作用时间呈正相关。4-萜烯醇与大肠杆菌DNA没有结合作用。【结论】 4-萜烯醇对大肠杆菌具有良好的抗菌效果，能通过增加细胞膜的通透性，进而使胞内容物泄漏使细菌死亡。试验结果为深入研究4-萜烯醇对大肠杆菌的抗菌机制奠定基础。

关键词: 4-萜烯醇; 大肠杆菌; 细胞膜; 抗菌作用

Abstract: 【Objective】 The experiment aimed to evaluate the antibacterial activity of terpinen-4-ol and provide support for its structural optimization and clinical application. 【Method】 Escherichia coli (E.coli) was selected as the model bacterium in this study.The minimum inhibitory concentration (MIC) of terpinen-4-ol against E.coli was determined by double dilution method.The antibacterial curve was measured by D_{600 nm} value.The effects of terpinen-4-ol on cell morphology of E.coli were analyzed by scanning electron microscopy.Then,the effect of terpinen-4-ol on E.coli cell membrane was evaluated by measuring the concentration of extracellular alkaline phosphatase (ALP) and β-galactosidase.Simultaneously,the leakage of nucleic acid and protein was determined using ultra micro spectrophotometer.The interaction of terpinen-4-ol with E.coli DNA was detected by nucleic acid gel electrophoresis. 【Result】 The MIC of terpinen-4-ol against E.coli was 0.013 mol/L.The antibacterial effect of terpinen-4-ol on E.coli was dose-dependent,and 1 MIC of terpinen-4-ol had good antibacterial effect within 24 h.E.coli treated with terpinen-4-ol became swelling and deformation,rough surface,and adhesion between bacterial bodies.Compared with control group,0.5 MIC of terpinen-4-ol could lead to a significant increase in the content of ALP in the supernatant of E.coli (P＜0.05) and an extremely significant increase in the content of β-galactosidase (P＜0.01),and the increase was positively correlated with the concentration of terpinen-4-ol.Compared with control group,0.5 MIC of terpinen-4-ol could also increase the contents of proteins and nucleic acids in the supernatant of E.coli,and it was positively correlated with the concentration of terpinen-4-ol and the duration of action.There was no binding between terpinen-4-ol and the E.coli DNA. 【Conclusion】 Terpinen-4-ol had a good antibacterial effect on E.coli,and exerted antibacterial effects by disrupting the permeability of cell membranes,and then leading to leakage of cellular contents and bacterial death,which laid a foundation for further research on the antibacterial mechanism of terpinen-4-ol on E.coli.

Key words: terpinen-4-ol; Escherichia coli; cell membrane; antibacterial effect

中图分类号:

周玲玲, 王宇航, 张元臣, 张明亮, 连凯琪. 4-萜烯醇对大肠杆菌的抗菌作用研究[J]. 中国畜牧兽医, 2025, 52(9): 4497-4504.

ZHOU Lingling, WANG Yuhang, ZHANG Yuanchen, ZHANG Mingliang, LIAN Kaiqi. Study on Antimicrobial Effects of Terpinen-4-ol Against Escherichia coli[J]. China Animal Husbandry & Veterinary Medicine, 2025, 52(9): 4497-4504.

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