硬脂酰辅酶A去饱和酶-1基因表达及其与鸭肉质和血清生化指标的相关性分析

doi:10.16431/j.cnki.1671-7236.2015.02.031

›› 2015, Vol. 42 ›› Issue (2): 432-437.doi: 10.16431/j.cnki.1671-7236.2015.02.031

硬脂酰辅酶A去饱和酶-1基因表达及其与鸭肉质和血清生化指标的相关性分析

张依裕^1,2, 李万贵^1,2

1. 贵州大学动物科学学院, 贵阳 550025;
2. 高原山地动物遗传育种与繁殖省部共建教育部重点实验室, 贵阳 550025

收稿日期:2014-07-21 出版日期:2015-02-20 发布日期:2015-02-13
通讯作者: 张依裕 E-mail:zyy8yyc@163.com
作者简介:张依裕(1976-),男,贵州黔西人,博士,硕士生导师,副教授,研究方向:动物遗传资源保护与利用
基金资助:
教育部博士点基金(20115201120004);国家自然科学基金(31160453)

Correlation Analysis between SCD-1 Gene Expression and Meat Quality and Serum Biochemical Indexes of Ducks

ZHANG Yi-yu^1,2, LI Wan-gui^1,2

1. College of Animal Science, Guizhou University, Guiyang 550025, China;
2. Key Laboratory of Animal Genetics, Breeding and Reproduction in the Plateau Mountainous Region, Ministry of Education, Guizhou University, Guiyang 550025, China

Received:2014-07-21 Online:2015-02-20 Published:2015-02-13

摘要/Abstract

摘要： 试验分别在基础日粮中添加含4%亚油酸和4%二十碳五烯酸(EPA)对樱桃谷鸭进行饲养,检测4、6、8和10周龄樱桃谷鸭硬脂酰辅酶A去饱和酶-1(SCD-1)基因mRNA表达丰度,分析其表达与10周龄鸭肉质和血清生化指标的相关性。实时荧光定量PCR结果显示,亚油酸和二十碳五烯酸均能显著降低各周龄SCD-1基因在鸭肝脏、脂肪和胸肌中的mRNA表达水平(P<0.05),说明亚油酸和二十碳五烯酸均具有抑制SCD-1基因转录的功能,亚油酸组鸭SCD-1基因mRNA表达水平与二十碳五烯酸组相比未达到显著差异(P>0.05);相关性分析结果显示,鸭SCD-1基因mRNA表达丰度与10周龄鸭血清中甘油三酯(TG)、总胆固醇(TCHO)、高密度脂蛋白(HDL)和低密度脂蛋白(LDL)呈显著正相关(P<0.05),与各常规肉质指标的相关性未达到显著水平(P>0.05),表明SCD-1基因在鸭的脂质代谢过程中发挥着重要的调控作用。

关键词: 鸭; SCD-1; 基因表达; 肉质; 血清生化指标

Abstract: In the present study,Cherry Valley ducks were subjected to diets with 4% linoleic acid and eicosapentaenoic acid (EPA) from week 2 to the end of week 10,respectively,and detected the mRNA expression of SCD-1 in 4,6,8 and 10 weeks,and analyzed its association with meat quality and serum biochemical indexes in 10 weeks.The Real-time fluorescence quantitative PCR results showed that the linoleic acid and EPA could significantly reduce the SCD-1 mRNA levels in liver,fat and breast muscles (P<0.05),meanwhile,the SCD-1 gene mRNA expression level had no significant difference between linoleic acid and EPA in every period (P>0.05).Correlation analysis showed that the mRNA expression levels of SCD-1 had significant positive correlation with serum TG,TCHO,HDL and LDL (P<0.05),and no significant correlation with all meat quality traits in 10 weeks (P>0.05).The research suggested that the transcription of SCD-1 gene was inhibited by linoleic acid and EPA,and played important role in the regulation of duck lipid metabolism.

Key words: duck; SCD-1; gene expression; meat quality; serum biochemical index

中图分类号:

S834

张依裕, 李万贵. 硬脂酰辅酶A去饱和酶-1基因表达及其与鸭肉质和血清生化指标的相关性分析[J]. , 2015, 42(2): 432-437.

ZHANG Yi-yu, LI Wan-gui. Correlation Analysis between SCD-1 Gene Expression and Meat Quality and Serum Biochemical Indexes of Ducks[J]. China Animal Husbandry & Veterinary Medicine, 2015, 42(2): 432-437.

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硬脂酰辅酶A去饱和酶-1基因表达及其与鸭肉质和血清生化指标的相关性分析

Correlation Analysis between SCD-1 Gene Expression and Meat Quality and Serum Biochemical Indexes of Ducks

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