China Animal Husbandry & Veterinary Medicine ›› 2014, Vol. 41 ›› Issue (10): 205-209.

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Effect of Different Factors on in Vitro Maturation of Porcine Oocytes

LIN Xiao-lu1, LIN Xiu-jiao1, WANG Xiu-ai2, LIU Feng-jun3, ZHUANG Yi-fen1, ZHANG Xing-feng1, LI Hong-xiang1, HONG Zhi-yong1, LIU Fang1, ZHANG Wen-chang1   

  1. 1. College of Animal Science, Fujian Agriculture and Forestry University, Fuzhou 350002, China;
    2. Fuqing Yongcheng Livestock Co., Ltd., Fuqing 350319, China;
    3. College of Animal Science and Technology, Henan University of Science and Technology, Luoyang 471003, China
  • Received:2014-04-08 Online:2014-10-20 Published:2014-10-29

Abstract: Effects of several factors such as exogenous hormones and ITS,culture methods,paraffin oil on in vitro maturation (IVM) for porcine oocytes were studied.The results indicated that,the group supplemented with PMSG+HCG+FSH was significantly higher than FSH+LH and HMG in mature media with regard to the mature rates of porcine oocytes (80.1% vs 68.3% vs 53.3%,P<0.05);the mature rate of porcine oocytes cultured in mature media supplemented with 1% ITS was not improved,while the cleavage rate and blastocyst rate of parthenogenesis embryos were significantly increased (63.3% vs 55.1%,18.7% vs 12.1%,P<0.05);the groove dish group was significantly higher than four-hole plate and 30 mm plastic dish group in terms of the mature rates of porcine oocytes (73.3% vs 68.0% vs 68.3%,P<0.05);mature media supplemented with paraffin oil was significantly increased the cumulus expansion and maturation rates for porcine oocytes (84.8% vs 69.9%,P<0.05). The results showed that the mature rates of porcine oocytes cultured in groove dish,supplemented with paraffin oil,PMSG+HCG+FSH and ITS were significantly improved.

Key words: porcine oocytes; in vitro maturation (IVM); culture methods; paraffin oil; exogenous hormones; ITS

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