芦丁对牛精液冷冻保存效果的影响

doi:10.16431/j.cnki.1671-7236.2026.01.028

Abstract

Abstract:

Objective This study aimed to investigate the effects of adding different concentrations of rutin to the diluent on the cryopreservation of bull semen. Method Semen was collected from three Simmental bulls and three Wagyu bulls using an artificial vagina. Samples with motility >75% were pooled and divided into five groups： A control group without rutin and four treatment groups supplemented with 0.4， 0.8， 1.2 and 1.6 g/L rutin in the diluent. Post-thaw sperm motility and kinematic parameters （including average path velocity， curvilinear velocity， straight-line velocity， wobble， linearity and straightness） were evaluated using a computer-assisted sperm analysis （CASA） system. Sperm malformation rate was assessed by eosin staining. Plasma membrane integrity was determined using the hypo-osmotic swelling test （HOST）. Acrosome integrity was examined by peanut agglutinin staining. Reactive oxygen species （ROS） level was measured by fluorescence assay. Antioxidant enzyme activities were also determine using the reagent kit. Result 0.8 g/L rutin group showed significantly higher post-thaw sperm motility， curvilinear velocity， straight-line velocity， wobble， linearity， plasma membrane integrity and acrosome integrity compared with control and other treatment groups （P<0.05）， and higher average path velocity and straightness compared with control group （P<0.05）. 0.8 g/L rutin group exhibited significantly lower sperm abnormality， ROS levels and malondialdehyde （MDA） content compared with control group （P<0.05）. The activities of catalase （CAT） and glutathione peroxidase （GSH-Px） in 0.8 and 1.2 g/L rutin groups were significantly higher than in control group （P<0.05）. Total antioxidant capacity （T-AOC） was significantly increased in 0.8 g/L rutin group compared with all other groups （P<0.05）， and 1.2 and 1.6 g/L rutin groups also showed significantly higher T-AOC than in control group （P<0.05）. Conclusion The addition of 0.8 g/L rutin to the diluent provided the best cryoprotective effects on bull semen， significantly improving post-thaw sperm motility， kinematic performance， plasma membrane and acrosome integrity and antioxidant enzyme activities （T-AOC， GSH-Px and CAT）， while significantly reducing sperm abnormality， ROS level and MDA content.

Key words: rutin; semen; cryopreservation; semen quality

CLC Number:

S823.8

LIU Hao, LU Jiahui, LIU Hongyu, ZHANG Yunhai, SONG Ning. Effects of Rutin on the Cryopreservation of Bovine Semen[J]. China Animal Husbandry & Veterinary Medicine, 2026, 53(1): 310-316.

Figures/Tables 5

Table 1

Table 2

Fig.1

Table 3

Table 4

References 25

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芦丁浓度 Rutin concentration/（g/L）	精子活力 Sperm motility/%	曲线速度 Curvilinear velocity/（μm/s）	直线速度 Straight-line velocity/（μm/s）	平均路径速度 Average path velocity/（μm/s）	线性度 Linearity/%	前向性 Straightness/%	摆动性 Wobble/%
0	38.19±0.78^c	40.88±0.97^c	17.30±0.67^b	23.62±1.64^b	17.50±0.43^b	33.61±1.02^c	27.09±1.23^b
0.4	42.76±0.91^b	51.13±1.96^b	18.60±0.57^b	26.60±0.52^ab	20.63±0.93^b	41.88±1.16^b	30.08±1.20^b
0.8	61.34±1.96^a	61.92±2.10^a	29.23±4.31^a	34.45±3.06^a	28.64±3.43^a	50.40±3.20^a	40.04±3.27^a
1.2	37.89±0.40^c	47.98±0.16^b	16.97±0.04^b	26.90±3.60^ab	18.84±0.38^b	40.18±0.42^b	29.14±0.19^b
1.6	39.18±0.79^c	48.31±0.26^b	18.86±0.76^b	29.66±2.60^ab	22.52±1.77^b	45.71±2.06^ab	32.19±1.25^b

芦丁浓度 Rutin concentration/（g/L）	精子畸形率 Sperm malformation rate/%
0	14.81±1.21^ab
0.4	15.73±0.50^a
0.8	10.95±0.20^c
1.2	13.53±0.32^b
1.6	14.10±0.14^ab

芦丁浓度 Rutin concentration/（g/L）	质膜完整性 Plasma membrane integrity/%	顶体完整性 Acrosome integrity/%
0	19.34±0.49^e	50.56±0.18^c
0.4	21.23±0.69^d	54.16±0.89^c
0.8	46.67±0.66^a	67.97±0.10^a
1.2	31.63±0.54^c	61.57±2.55^b
1.6	34.47±0.32^b	58.21±0.56^b

芦丁浓度 Rutin concentration/ （g/L）	活性氧 ROS/（mmol/L）	丙二醛 MDA/（μmol/L）	过氧化氢酶 CAT/（U/mL）	谷胱甘肽过氧化物酶 GSH-Px/（U/mL）	总抗氧化能力 T-AOC/（U/mL）
0	50.37±6.01^a	126.61±5.67^a	15.03±1.09^c	1.10±0.10^b	107.84±0.61^d
0.4	48.70±0.26^ab	81.30±3.74^b	12.52±0.84^c	1.09±0.16^b	107.68±0.41^d
0.8	46.20±2.85^b	67.20±4.48^c	34.83±2.41^a	2.11±0.12^a	122.12±1.02^a
1.2	48.20±0.45^ab	93.28±2.01^b	23.07±2.23^b	1.79±0.12^a	115.93±0.46^b
1.6	49.26±0.62^ab	115.05±3.39^a	17.62±1.99^bc	1.33±0.58^b	111.53±0.60^c

Effects of Rutin on the Cryopreservation of Bovine Semen

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