9型猪链球菌的分离鉴定及其生物学特性研究

doi:10.16431/j.cnki.1671-7236.2025.10.029

Abstract

Abstract: 【Objective】 The purpose of this study was to understand the biological characteristics and pathogenicity of the isolates of Streptococcus suis serotype 9 in Guangdong,and provide a reference for the prevention and treatment of the disease caused by it.【Method】 In this experiment,bacterial isolation and culture were carried out with spleen samples from a pig with porcine reproductive and respiratory syndrome (PRRS) in a pig farm in Guangdong,and the isolated strain was identified by morphological observation,Gram staining microscopy,biochemical tests,16S rRNA gene sequencing and serotype identification.The biological characteristics and pathogenicity of the isolated strain were analysed by virulence gene detection,drug sensitivity test and mouse infection test.【Result】 The isolated strain showed rounded colonies of greyish-white,moist,smooth,and neat edges on blood plate,characteristic of alpha-haemolysis.Gram staining microscopic examination showed purple and was arranged in a circular chain pattern.Sequencing results of the 16S rRNA gene of the isolate showed more than 99% similarity with the published strains of Streptococcus suis on NCBI,and serotyping results showed that the isolate was Streptococcus suis serotype 9.The virulence gene phenotype of the isolated strain was luxS^-/ccpA⁺/srtA⁺/mrp^-/gdh⁺/gapdh⁺/fbps⁺/epf^-/orf2⁺/sly^-.The isolated strain was resistant to five antibiotics:Macrolide,lincomycin,doxycycline,tetracycline and sulfadimethoxazole.The results of pathogenicity test showed that the LD₅₀ of the isolate to mice was 1×10¹⁰ CFU,and the infected mice exhibited different degrees of lesions in organs,such as liver,kidney,spleen,lung,heart and brain.【Conclusion】 In this study,a strain of Streptococcus suis serotype 9,which carried multiple virulence genes was isolated,it was resistant to a variety of antibiotics,and could cause different degrees of tissue damage in infected mice.The results of this study could provide a reference basis for the prevalence analysis and prevention and control of Streptococcus suis infection.

Key words: Streptococcus suis serotype 9; isolation and identification; biological characteristics; pathogenicity

CLC Number:

S852.61

WU Jiandong, KANG Huahua, ZHAI Shaolun, YANG Dongxia, YANG Nanling, GOU Hongchao, JIA Aiqing, LI Xiaojun, LI Chunling, LI Yan. Isolation and Identification of Streptococcus suis Serotype 9 and Its Biological Characteristics Analysis[J]. China Animal Husbandry & Veterinary Medicine, 2025, 52(10): 4844-4853.

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Isolation and Identification of Streptococcus suis Serotype 9 and Its Biological Characteristics Analysis

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